联苯胺亲和层析纯化尿激酶

利用甲基丙烯酸缩水甘油酯与乙二醇甲基丙烯酸酯经悬浮聚合反应合成亲水性亲和载体骨架,骨架上的不环氧基与氨水反应,继之与丁二酸酐反应,最后与配基联苯胺偶联制备亲和载体,配基密度为５４μｍｏｌ／ｇ湿胶。亲和载体吸附激酶的最佳条件为ＰＨ４．６和２．５ｍｏｌ／ＬＮａＣｌ;测定了吸附等温线,算得离常数Ｋｄ为３１６ＩＵ／ｍｌ,了大吸附量ｑｍ为２．３８３×１０＾４Ｕ／ｍＬ湿胶,洗脱条件为含０．５ｍｏｌ／ＬＮａＣｌ     

ConA—Sepharose 4B的制备及其对血浆糖蛋白的亲和效率

报道了ＣｏｎＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ的制备方法，经测定Ｓｅｐｈａｒｏｓｅ４Ｂ对ＣｏｎＡ的偶联效率达８５％，该亲和凝胶对血浆糖蛋白有亲和吸附．其亲和效率为１．８ｍｇ（蛋白／ｍｌ凝胶）．     

聚苯乙烯载体亲和层析纯化尿激酶

聚苯乙烯经磺化后连接手臂６－氨基己酸，然后偶联配基对氨基苯甲脒，制成亲水性的亲和载体。载体的最适配基密度为每克湿载体３２μｍｏｌ，最适吸附条件为ｐＨ７．５、０．０５ｍｏｌ／Ｌ磷酸缓冲液（含ＮａＣｌ０．５ｍｏｌ／Ｌ）。在ｐＨ４．０、０．１ｍｏｌ／Ｌ醋酸缓冲溶液（含ＮａＣｌ０．５ｍｏｌ／Ｌ）中，采用０～１ｍｏｌ／Ｌ　ＮａＳＣＮ梯度洗脱，可将比活８．３３μｍｏｌ·Ｓ－１·ｍｇ－１的粗酶纯化５０倍，收率大于５３％。     

用锌离子从人尿中分离尿激酶

用低浓度锌离子从人尿中沉淀尿激酶（ＵＫ）,锌离子浓度为３ｍｍｏｌ／Ｌ时,沉淀回收率为８７％～９３％,用ｐＨ１０～１０．５的２０ｇ／Ｌ硫酸铵按１∶４（ｇ∶ｍｌ）溶解ＵＫ沉淀物,ＵＫ溶解得率为９７％。溶解液用７０％饱和度的硫酸铵盐析,ＵＫ盐析收率为９１％,粗品纯度为４．０８×１０５ＩＵ／ｇ,固体效价为３．６×１０４ＩＵ／ｇ,总收率在７７％～８２％之间。每吨尿（５．００×１０６ＩＵ计）可得ＵＫ粗品（３．８～４．１）×１０６ＩＵ。该粗品可用离子交换层析或亲和层析进一步纯化。     

特异性Tetrodotoxin亲和吸附微珠的制备及其提取性能

将横纹东方豚卵巢与醋酸乙醇溶液混合匀浆,经离心,加热,沉淀,脱脂等处理,制得粗提液,活性炭吸附粗提液得到粗毒液;以壳聚糖为载体合成亲和微珠分别来分离纯化粗提液和粗毒液,用传统的小鼠生物法检测TTX毒素量．潮湿活性炭对TTX的吸附量约为2μg／g,潮湿亲和微珠吸附量约为10μg／g;亲和微珠吸附提取粗毒液,回收率达51％,直接吸附粗提液,回收率达到80％,同时蛋白也得到有效的去除．     

里氏木霉诱导合成木聚糖酶的调控

提出了两种不同用途的木聚糖酶的诱导合成方法。以里氏木霉为产酶菌,经适当处理后的玉米芯可诱导产生含纤维素酶（３．４ＩＵ／ｍＬ）的高活力木聚糖酶（５４．４ＩＵ／ｍＬ）。以混有少量纤维素的粗木聚糖作碳源,通过分批补料及对培养条件的限制性控制里氏木霉可选择性合成木聚糖酶;选择性合成程度与碳源浓度有关,当碳源浓度为１０ｇ／Ｌ时木聚糖酶和纤维素酶活力分别为３５．５ＩＵ／ｍＬ、０．２Ｕ／ｍＬ,两种酶活的比值达１７７．５。     

两种不同的亲和吸附剂对猪凝血酶纯化效果的比较

以壳聚糖为载体、肝素为配基和以琼脂糖4B为载体、肝素为配基分别合成2种亲和吸附剂,用以纯化猪凝血酶,并对两者的纯化效果进行比较.结果表明对于猪凝血酶的分离提纯,前者明显优于后者.前者的酶比活为1553.7U*mg-1,提纯11.5倍,收率为78.8%;后者的酶比活为1126.4U*mg-1,提纯8.8倍,收率为61.4%.前者无明显压床,流速均匀,而且前者的价格低廉,易于得到.     

单克隆抗体亲和层析法纯化人尿激酶

含抗人尿激酶单克隆抗体的腹水经ＰｒｏｔｅｉｎＡ－ＳｅｐｈａｒｏｓｅＣＬ４Ｂ柱纯化得到ＩｇＧ纯品,分子量为１５０ｋＤ（重链５５ｋＤ,轻链２０ｋＤ）,与ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４Ｂ偶联制成抗人尿激酶单克隆抗－体－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析介质。尿激酶粗品经单抗－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱纯化后,所得尿激酶制品比活为９×１０－４～１．７×１０－３ｍｏｌ／ｓ·ｍｇ－１,纯化倍数为１５～３０倍,酶活性回收率在５０％以上。     

人尿胰蛋白酶抑制剂的分离纯化

人尿经吸附剂处理，硫酸铵沉淀后，得到胰蛋白酶抑制剂粗品。粗品经ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０层析及ｔｒｙｐｓｉｎ－Ｓｅｐｈａｒｏｓｅ亲和层析，得到两种ＨＵＴＩ：ＨＵＴＩ１与ＨＵＴＩ２。ＨＵＴＩ１的比活为３５５６ｕｎｉｔｓ／ｍｇ，活性回收率为３５％，含分子量６７０００与２５０００两种组粉；ＨＵＴＩ２的比活为３３８５ｕｎｉｓ／ｍｓ，活性回收率为３１％，分子量为６７０００。经二步层析纯化，纯化因子达     

啤酒废酵母超氧化物歧化酶（SOD）的分离纯化及性质研究

啤酒废酵母（１０８－１０９细胞／ｍｌ）通气发酵后，经乙醇－氯仿沉淀，加热，调ＰＨ，（ＮＨ４）２ＳＯ４沉淀等除去杂蛋白质。最后经ＳｅｐｈａｄｅｘＧ－７５层析得到纯化的ＣｕＺｎ－ＳＯＤ，酶比活在１５００单位／ｍｇ蛋白以上，酶的分子量为１４８０００，由四个亚基组成，紫外最大吸收峰位于２５６ｕｍ处。Ａ２５６．４／２８０＝１．２２．     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Jane Eyre——A Feminist In The 19th Century

In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.     

Editorial note for the special topic on ＂Nanopore Analysis＂

The concept of nanopore analysis, using the pore-forming protein a-hemolysin to detect individual nucleic acids at a single-molecule level, was first proposed in 1996. Over the past two decades, tremendous progress has been made in the nanopore field, and nanopore analysis has become a label-free and high-throughput method for probing bio- molecules and other analytes with single-molecule sensi- tivity, especially holds the promising for ＂third generation＂ DNA sequencing. However, challenges still remain in the experimental strategies and the design of whole nanopore-based instruments. Here, we proudly present a special topic dedicated to the topic of ＂Nanopore Analysis＂, with 8 reviews/articles providing up to date coverage of the experimental strategies, theoretical calcu- lations and simulations, and instrument design. Reviews and articles on the experimental strategies cover control of DNA partitioning into a nanopore, detection of target DNA, and the advantages of nanopore-based DNA sequencing. The theoretical calculations and simulations discuss the translocation behavior of DNA, and an inte- grated measurement system and data analysis software are presented for instrument design.     

Effects of climate change on biodiversity

1 Rise of studies on climate change＇s effects on biodiversity
Until the 1980s, climate change and biodiversity were studied as two independent disciplines for more than a century. In 1992, the Ecological Society of America＇s annual report named climate change, biodiversity, and the sustainable ecological system as the three major global environmental issues of the twenty-first century [1].     

Discussing the Expansive Learning in Modern Organizations

<正>1 Introduction Endeavours on learning methods have long occupied the mind spaces of scholars and there have been theories abound on how people learn and in what way organisations can use some of the knowledge gained in the art of learning to maximise human productivity.While the theories by themselves are not solutions to problems,they are steps of inquiry that provide us with interesting perspectives that we can use to extend our knowledge of the dimensions of learning.This paper is an exploratory work on the theories postulated by three notable scholars including Zimmerman     

Progress in Linear Scaling Non-Orthogonal Localized Molecular Orbitals Method

The non-orthogonal localized molecular orbital(NOLMO)is the most localized representation of electronic degrees of freedom.As such,NOLMOs are thus potentially the most efficient for linear scaling calculations of electronic structures for large systems.However,direct ab initio calculations with NOLMO have not been fully implemented and widely used,partly because of the slow convergence issue in the optimization of NOLMO.We devel-     

An Accurate and Universal HEOM Approach to Strongly Correlated Quantum Impurity Systems

A hierarchical equations of motion(HEOM)approach is developed for general open quantum systems coupled to fermionic environment.The HEOM method is in principle formally exact,as it resolves nonperturbatively the combined effects of many-body interaction,system-bath dissipation,and non-Markovian memory.In practice,the HEOM approach is highly accurate and efficient for the characterization of strongly correlated quantum impurity sys-     

关于有理混合吸引子的Ciric型非唯一不动点定理

本文我们应用有理混合吸引子条件去证明具非唯一不动点的映射Ciric型的若干不动点定理.结果推广并改进已知的一些结果.     

次线性算子交换子在变指数Herz-Morrey空间上的有界性

本文主要利用给出的次线性算子分别与BMO函数及Lipschitz函数生成的交换子在变指数L~(p(·))(R~n)空间上的有界性,证明了其在变指数Herz-Morrey空间MK_(q,p(·))~α~((·)),λ(R~n)上的有界性.     

利用GPS双频观测值实现PPP电离层延迟高阶项改正

给出了利用双频观测值计算L3组合电离层延迟高阶项改正的方法,并与全球电离层延迟文件的改正效果进行对比.利用赤道附近的15个国际全球卫星导航定位系统服务组织(IGS)站的数据进行比较,结果表明:2种方法计算的电离层二阶项延迟互差最大不超过1 cm,三阶项延迟互差最大不超过5 mm;电离层高阶项改正后的观测值精密单点定位(PPP)解算结果N、E、U方向互差平均值分别为0.4、0.5、1.0mm,因此2种改正方法效果在同一水平.