竹叶青（Trimeresurus stejnegeri）蛇毒

用ＤＥＡＥ－Ｓｅｐｈａｄｅｘ－Ａ－５－分离竹叶青粗毒，再经ＣＭ－Ｓｅｐｈａｒｏｓｅ－ＣＬ－６Ｂ、ＱＡＥ－Ｓｅｐｈａｄｅｘ－Ａ－５０和ＳｅｐｈａｄｅｘＧ－１００纯化，得到一个５－核苷酸酶，对血小板聚织要具有强抑制作用，在ＳＤＳ－聚丙烯酰谘电泳图谱上为一单一蛋白带，分子量为８２５００，是一个糖蛋白，含６．７％中性糖和０．７％唾液酸。     

环毛蚓纤维素酶C_X的初步研究

从环毛蚓（Ｐｈｅｖｅｔｉｍａａｓｉａｔｉｃａ）提取纤维素酶ＣＸ,用ＳｅｐｈａｄｅｘＧ５０凝胶过滤和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ２５离子交换层析进行分离纯化,得ＣＸⅠ和ＣＸⅡ,分别测定了ＣＸ,ＣＸⅠ和ＣＸⅡ的最适ｐＨ和最适温度．     

花椰菜凝集素的纯化及部分性质的研究

花椰菜凝集素由花蕊组织经生理盐水抽提、硫酸铵沉淀和ＳｅｐｈａｄｅｘＧ－１００、Ｓｅｐｈａｒｏｓｅ－４Ｂ凝胶层析纯化获得．ＰＡＧＥ鉴定和高碘酸－Ｓｃｈｉｆｆ试剂反应均显单一条带，说明该凝集素为纯化的糖蛋白．经ＳｅｐｈａｄｅｘＧ－２００柱过滤分析，其相对分子质量约为９４０００．ＳＤＳ－ＰＡＧＥ分析表明该凝集素含有４个亚基，其相对分子质量分别约为２８０００、２５０００、２２０００和２００００．花椰菜凝集素经５０℃处理５分钟仍保持高的凝集活性，该凝集素对酸处理较碱处理稳定．     

栽培和野生大豆核酮糖—1,5—二磷酸羧化酶的结晶及其大小亚基等 …

栽培大豆和野生大豆叶片的蛋白抽提液经硫酸铵盐析,Ｓｅｐｈａｄｅｘ Ｇ－５０柱层析以及浓缩透析等步骤,分别得到核酮糖－１,５－二磷酸羟化酶的结晶。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ酶标抗体技术鉴定证实为Ｒｕｂｉｓｃｏ。用ＩＥＦ－ＰＡＧＥ方法分析这２种不同进化类型大豆的Ｒｕｂｉｓｃｏ大小亚基,发现其具有高度同源性。     

栽培和野生大豆核酮糖-1,5-二磷酸羧化酶的结晶及其大小亚基等电聚焦分析

栽培大豆和野生大豆叶片的蛋白抽提液经硫酸铵盐析、Ｓｅｐｈａｄｅｘ Ｇ－５０柱层析以及浓缩透析等步骤,分别得到核酮糖－１,５－二磷酸羟化酶（简称Ｒｕｂｉｓｃｏ）的结晶．经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ酶标抗体技术鉴定证实为Ｒｕｂｉｓｃｏ．用ＩＥＦ－ＰＡＧＥ方法分析这２种不同进化类型大豆的Ｒｕｂｉｓｃｏ 大小亚基,发现其具有高度同源性     

黑曲霉胞外葡萄糖淀粉酶的纯化及特性

黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒ７２８）的粗酶液，经硫酸铵沉淀，ＳｅｐｈａｄｅｘＧ－２５柱凝胶过滤脱盐，ＤＥＡＥ－Ｔｏｙｏｐｅａｒｌ离子交换柱层析和ＳｅｐｈａｃｒｙＳ－１００凝胶层析纯化，经ＰＡＧＥ鉴定为一条带ＳＤＳ凝胶电泳测定分子量为７００００．金属离子Ｆｅ３＋对该酶有一定的抑制作用，该酶的等电点为３．７，最适ｐＨ为４，最适温度为６０℃．Ｅ２８０１％为１５．７２，Ｋｍ值为０．１１２×１０－３ｍ．     

福寿螺β-葡萄糖苷酶的分离纯化及性质的初步研究

以福寿螺内脏为材料,经本实验室开发的工业生产法制备粗酶粉,进一步采用葡聚糖凝胶（ＳｅｐｈａｄｅｘＧ－２００）和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析纯化,获得经ＳＤＳ－聚丙烯酰胺凝胶电泳鉴定为单一纯的β－葡萄糖苷酶制剂．测定该酶的最适反应温度为４３℃,最适反应ｐＨ为５．１;在ｐＨ５．１,４５℃下,该酶催化水杨素水解的Ｋｍ值为３．４０ｍｍｏｌ／Ｌ,活化能为５０．６３ｋＪ／ｍｏｌ．研究几种效应物对酶活力的影响,结果表明：Ｍｎ２＋、Ｃｏ２＋对酶有激活作用,而Ｃｕ２＋、Ｈｇ２＋、Ｐｂ２＋、ＳＤＳ及脲对酶有不同程度的抑制作用,其中Ｃｕ２＋和Ｈｇ２＋对该酶的抑制作用最强,其抑制机理均表现为非竞争性抑制     

长白山白眉蝮蛇蛇毒精氨酸酯酶的荧光淬灭研究

交替使用ＤＥＡＥＳｅｐｈａｄｅｘ Ａ－ ５０ 离子交换和Ｓｅｐｈａｄｅｘ Ｇ－ ７５ 凝胶过滤层析技术, 从长白山白眉蝮蛇蛇毒（ Ａｇｋｉｓｔｒｏｄｏｎ ｂｌｏｍｈｏｆｆｉｉ Ｕｓｓｕｒｅｎｓｉｓ） 中纯化得到了一种分子量为２９４５８ ±１５Ｄａ 的精氨酸酯酶活性组分． 本文研究了碘离子（Ｉ－ ） 和丙烯酰胺（Ａｃｒ） 对这种精氨酸酯酶的荧光光谱的淬灭作用,Ｉ－ 和Ａｃｒ 对精氨酸酯酶荧光的淬灭符合Ｓｔｅｒｎ － Ｖｏｌｍｅｒ 公式． Ｉ－ 仅能淬灭精氨酸酯酶约５４ ％ 的荧光, Ａｃｒ 可以将精氨酸酯酶荧光淬灭殆尽． Ｉ－ 和Ａｃｒ 对长白山白眉蝮蛇蛇毒精氨酸酯酶荧光淬灭作用的结果表明： 在精氨酸酯酶中含有两种类型的色氨酸残基, 而且处于不同的微环境当中．     

人型结核杆菌H37Pv株菌体特异性的抗原TB4单克隆抗体？…

采用硫酸铵法，辛酸二步法，ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ５０离子交换柱层析法，从杂交瘤小鼠腹水中纯化人型结核杆菌Ｈ３７Ｒｖ株特异性抗原ＴＢ４的单克隆抗体，从回收率、纯度和活性３个方面进行了比较。     

人红细胞生成素受体膜外结构域在大肠杆菌中的融合表达及其纯化

将插入ｐＢｌｕｅｓｃｒｉｂｅ的人红细胞生成素受体ｃＤＮＡ,用ＥｃｏＲＩ和ＢａｍＨＩ酶解,分离得到的基因片段,插入到经ＥｃｏＲＩ和ＲａｍＨＩ消解的表达载体ｐＧＥＸ－３Ｘ中,得到重组表达质粒ｐＧＥＸ－３Ｘ／ｈＥＰＯＲ。重组质粒ｐＧＥＸ－３Ｘ／ｈＥＰＯＲ含有ｔａｃ启动子,ＥＰＯＲ膜外结构域基因５端与谷胱甘肽转移酶（ＧＳＴ）编码基因融合,阳性重组子在大肠杆菌中经ＩＰＴＧ诱导表达ＧＳＴ－ｈＥＰＯＲ,重组表达菌裂解上清液经ＧＳＨ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和柱一步纯化,能除去绝大部分杂蛋白,基本达到纯化的效果。ＳＤＳ－ＰＡＧＥ和免疫杂交分析显示,重组表达产物确系人红细胞生成素受体     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.