嗜盐菌碱性淀粉酶特性研究

碱性嗜盐菌ＨａｌｏｂａｃｔｅｒｉｕｍｓｐＨ－３７１产生的淀粉酶经超滤、硫酸铵沉淀和ＤＥＡＥ－纤维素纯化后，ＳＤＳ－ＰＡＧＥ凝胶电泳为一条带，分子量为２７０００，等电点３．７，最适反应ｐＨ９．０，最适反应温度６５℃．ＮａＣｌ是维持酶活性的必需因子，酶解产物为麦芽糖、麦芽三糖、麦芽四糖和麦芽五糖     

绿色乳杆菌α－羟基戊二酸脱氢酶的纯化和特性研究

本文对绿色乳杆菌（Ｌ．Ｖｉｒｉｄｅｓｃｅｎｓ）Ｂ１７５产生的α－羟基戊二酸脱氢酶用硫酸铵盐析；ＤＥＡＥ—纤维素柱层折；超滤浓缩；苯基琼脂糖凝胶（ＰｈｅｎｙｌＳｅｐｈａｒｏｓｅ）疏水层析等方法纯化，使酶的纯度提高１１７倍。通过ＨＰＬＣ和等电聚焦测得该酶的分子量约为６６，０００，等电点为４．２。酶催化作用的最适ｐＨ５．５，最适温度为６０℃，对α—酮戊二酸和ＮＡＤＨ的Ｋｍ值分别为０．１４×１０（－３）Ｍ和０．０３×１０（－３）Ｍ。     

福寿螺β-葡萄糖苷酶的分离纯化及性质的初步研究

以福寿螺内脏为材料,经本实验室开发的工业生产法制备粗酶粉,进一步采用葡聚糖凝胶（ＳｅｐｈａｄｅｘＧ－２００）和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析纯化,获得经ＳＤＳ－聚丙烯酰胺凝胶电泳鉴定为单一纯的β－葡萄糖苷酶制剂．测定该酶的最适反应温度为４３℃,最适反应ｐＨ为５．１;在ｐＨ５．１,４５℃下,该酶催化水杨素水解的Ｋｍ值为３．４０ｍｍｏｌ／Ｌ,活化能为５０．６３ｋＪ／ｍｏｌ．研究几种效应物对酶活力的影响,结果表明：Ｍｎ２＋、Ｃｏ２＋对酶有激活作用,而Ｃｕ２＋、Ｈｇ２＋、Ｐｂ２＋、ＳＤＳ及脲对酶有不同程度的抑制作用,其中Ｃｕ２＋和Ｈｇ２＋对该酶的抑制作用最强,其抑制机理均表现为非竞争性抑制     

黑曲霉 A3 木聚糖酶的纯化和性质

黑曲霉Ａ３（ＡｓｐｅｒｇｉｌｕｓｎｉｇｅｒＡ３）为高产木聚糖酶菌株。该菌所产生的木聚糖粗酶经硫酸铵、乙醇沉淀,ＳｅｐｈａｄｅｘＧ－１００和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０分离纯化后,获得３个组分,分别称为Ｘ１、Ｘ２、Ｘ３。它们的最适反应温度和ｐＨ依次为４０、５０、５０℃和３５、４５、５０。     

染料脱色酶特性研究

从脱色优势菌ＮＤ１ 提取脱色酶,对酶特性进行研究．结果表明酶的最适作用条件为ｐＨ ７,３７℃,无氧环境,反应需ＮＡＤＨ 的参与;纯化结果表明经ＤＥＡＥ柱层析及Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析后脱色速率分别提高１０ 倍和５０倍,Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析中２～４ 管蛋白具有偶氮和蒽醌染料脱色酶活性,６～１２ 管具有蒽醌染料脱色酶活性．     

花椰菜凝集素的纯化及部分性质的研究

花椰菜凝集素由花蕊组织经生理盐水抽提、硫酸铵沉淀和ＳｅｐｈａｄｅｘＧ－１００、Ｓｅｐｈａｒｏｓｅ－４Ｂ凝胶层析纯化获得．ＰＡＧＥ鉴定和高碘酸－Ｓｃｈｉｆｆ试剂反应均显单一条带，说明该凝集素为纯化的糖蛋白．经ＳｅｐｈａｄｅｘＧ－２００柱过滤分析，其相对分子质量约为９４０００．ＳＤＳ－ＰＡＧＥ分析表明该凝集素含有４个亚基，其相对分子质量分别约为２８０００、２５０００、２２０００和２００００．花椰菜凝集素经５０℃处理５分钟仍保持高的凝集活性，该凝集素对酸处理较碱处理稳定．     

解脂假丝酵母胞外脂肪酶的提纯和性质

通过ＤＥＡＥ－ＣｅｌｌｕｌｏｓｅＤＥ－５２和ＤＥＡＥ－ＳｅＰｈａｄｅｘＡ－５０离子交换层析等提纯步骤，对解脂假丝酵母胞外脂肪酶进行了纯化，得到了层析纯样品，比活提高２７．４倍，得率１１％．该酶反应最适温度为４０℃；最适ＰＨ为７．５；等电点约在ＰＨ４．５～５．０之间；８℃以下存放，酶活力损失较少，３０℃以上存放，酶活力有明显损失．金属离子Ｓｒ２＋，Ｂａ２＋，Ｚｎ２＋和Ｍｇ２＋对该酶的激活作用为Ｓｒ２＋＞Ｂａ２＋＞Ｚｎ２＋＞Ｍｇ２＋．Ｃｕ２＋和Ｎａ＋对酶活性有抑制作用．ＮａＮ３对保持酶活性有重要作用．     

背角无齿蚌酸性磷酸酶的分离、纯化及部分性质研究

从背角无齿蚌外套膜中,经盐析,ＤＥＡＥ－Ｓｅｐｈａｄｅｘ Ａ－５０离子交换柱层析及Ｓｅｐｈａｄｅｘ,Ｇ－１５０凝胶过滤等步骤,分离提纯到一种酸性磷酸酶,提纯倍数８８．２５酶液比活力为２４．７Ｕ／ｍｇ,通过动力学方法测得其最适ｐＨ值为４．８,最适温度为５０℃,同样以对硝基苯磷酸二钠作底物测得其Ｋｍ值为０．７３ｍｍｏｌ／Ｌ ,Ｚｎ＾２＋对酶有激活作用,而Ｆ＾－,Ｐｂ＾２＋和Ｂａ＾２＋则对酶有一定的抑制     

普鲁兰酶的纯化及其性质

采用ＤＥＡＥ—纤维素柱和ＳｅｐｈａｄｅｘＧ－１００柱色谱对从土壤中筛选得到的普鲁兰杆菌（Ｂａｃｉｌｕｓａｃｉｄｏｐｕｌｕｌｙｔｉｃｕｓ）菌株所产生的普鲁兰酶（丹麦ＮｏｖｏＮｏｒｄｉｓｋ产品）进行分离纯化。酶活测定的结果表明,该酶的最适催化ｐＨ为４８,最适温度为３８℃,有相当高的热稳定性,米氏常数Ｋｍ为５２ｍｍｏｌ。     

一种具有刺激红系细胞集落生成的螺旋藻（Spirulina…

从螺旋藻体中通过ＤＥＡＥ纤维素层析，硫酸铵分部沉淀和葡聚糖Ｇ７５凝胶过滤得到一种具有离体情况下刺激红系细胞集落生成的功能蛋白质。ＳＤＳ－ＰＡＧＥ测分子量为１５０００道尔顿，等电点为４．８，含有藻蓝胆素。该蛋白命名为螺旋藻１５０００蛋白（Ｓｐｉｒｕｌｉａ ｐｌａｔｅｎｓｉｓ１５０００，ＳＰ－１５０００）。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.