枯草杆菌AS1．398制备中性蛋白酶的研究

本文研究了枯草杆菌ＡＳ１．３９８发酵培养制备中性蛋白酶，对发酵培养基配比，培养条件，金属离子的影响等进行了摇瓶试验。制备的ＡＳ１．３９８中性蛋白酶有如下性质：最适ＰＨ７．２－７．４，在ＰＨ６．５－８．０稳定。最适温度４２－４４℃，３５℃下处理２小时能保持约８５％酶活力，６０℃下１０分钟酶完全失活，此酶被ＥＤＴＡ和Ｃｕ２＋所抑制     

棒曲霉UA—2木聚糖酶的蔗渣固态发酵

棒曲霉（Ａｓｐｅｒｇｉｌｕｓｃｌａｖａｔｕｓ）ＵＡ－２固态发酵蔗渣产木聚酶的培养基为每克蔗渣加营养液５ｍｌ，初始ｐＨ８．５．营养液的适宜组成为每升含：ＮＨ４ＮＯ３１０ｇ、Ｋ２ＨＰＯ４６ｇ、ＭｇＳＯ４·７Ｈ２Ｏ０．７５ｇ、ＣａＣｌ２０．７５ｇ、ＦｅＳＯ４·７Ｈ２Ｏ１１．２５ｍｇ、ＭｎＳＯ４·Ｈ２Ｏ３．７５ｍｇ、ＺｎＳＯ４３．０ｍｇ、ＣｏＣｌ２４．５ｍｇ．在上述培养基中接入其湿重１０％（Ｗ／Ｗ）的新鲜的ＵＡ－２种子曲，２８℃培养１０８ｈ，其木聚糖酶，滤纸降解酶和羧甲基纤维素酶的活力分别为２６９５．６、２８．５和４２．７ｕ／ｇ蔗渣．酶反应的最适ｐＨ５．０，最适温度５０℃；在ｐＨ４．０～１１．０内酶活性十分稳定．５０℃保温１ｈ，酶活剩余５５％，８℃下放置２３ｄ，活力几乎不变．磷酸盐，半胱氨酸对酶有激活作用，ＥＤＴＡ和对氯汞本甲酸对酶有抑制作用     

水、旱稻幼苗β－淀粉酶酶学性质的比较研究

以四种抗旱性不同的水、旱稻幼苗的茎、叶为实验材料，应用ＤＮＳ法测定β－淀粉酶的活性，比较抗旱性不同品种间β－淀粉酶酶学性质（包括酶的最适温度、热稳定性、抑制剂和激动剂对酶活性的影响以及Ｋｍ值）的差别．结果表明，所有供试品种β－淀粉酶的最适温度为４０℃；不抗旱的８３－８和８２－１β－淀粉酶的热稳定性强于抗旱的秦爱和７８－８－１０；ＥＤＴＡ和Ｃｕ２＋，Ｚｎ２＋，Ｍｇ２＋，Ｐｂ２＋和Ｈｇ２＋等金属离子是β－淀粉酶的抑制剂，Ｍｎ２＋，Ｂａ２＋，Ｃｏ２＋和Ｃａ２＋等金属离子β－淀粉酶的激动剂；旱稻品种β－淀粉酶的Ｋｍ值大于水稻．     

木聚糖降解菌的筛选和木聚糖酶性质的研究

筛选两株生长快、产木聚糖酶活力高的菌种；黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒ，ＡＮ０１）、链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓｓｐ．Ｓｔｒ７Ｂ），酶活力分别达１２８ｍｇ／Ｌ·ｍｉｎ和１７６ｍｇ／Ｌ·ｍｉｎ；酶反应最适温度分别为６０℃与５０℃；最适ｐＨ值为５．０和６．０，并分别在ｐＨ２．２～５．０，５．８～６．４酶活性稳定；在６０℃条件下保温１．５ｈ，酶活力分别剩余２０．５％，８８．５％，其中ＡＮ０１株原酶液在９０℃保温１０ｍｉｎ，活力仍剩余１４．５％．Ｃｕ２＋对酶活表现出极强抑制，Ｆｅ２＋，Ｍｇ２＋，Ｃａ２＋等离子则有促进作用；用纸层析法探讨了不同培养时间各种产物产生的情况．     

背角无齿蚌酸性磷酸酶的分离、纯化及部分性质研究

从背角无齿蚌外套膜中,经盐析,ＤＥＡＥ－Ｓｅｐｈａｄｅｘ Ａ－５０离子交换柱层析及Ｓｅｐｈａｄｅｘ,Ｇ－１５０凝胶过滤等步骤,分离提纯到一种酸性磷酸酶,提纯倍数８８．２５酶液比活力为２４．７Ｕ／ｍｇ,通过动力学方法测得其最适ｐＨ值为４．８,最适温度为５０℃,同样以对硝基苯磷酸二钠作底物测得其Ｋｍ值为０．７３ｍｍｏｌ／Ｌ ,Ｚｎ＾２＋对酶有激活作用,而Ｆ＾－,Ｐｂ＾２＋和Ｂａ＾２＋则对酶有一定的抑制     

福寿螺β-葡萄糖苷酶的分离纯化及性质的初步研究

以福寿螺内脏为材料,经本实验室开发的工业生产法制备粗酶粉,进一步采用葡聚糖凝胶（ＳｅｐｈａｄｅｘＧ－２００）和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析纯化,获得经ＳＤＳ－聚丙烯酰胺凝胶电泳鉴定为单一纯的β－葡萄糖苷酶制剂．测定该酶的最适反应温度为４３℃,最适反应ｐＨ为５．１;在ｐＨ５．１,４５℃下,该酶催化水杨素水解的Ｋｍ值为３．４０ｍｍｏｌ／Ｌ,活化能为５０．６３ｋＪ／ｍｏｌ．研究几种效应物对酶活力的影响,结果表明：Ｍｎ２＋、Ｃｏ２＋对酶有激活作用,而Ｃｕ２＋、Ｈｇ２＋、Ｐｂ２＋、ＳＤＳ及脲对酶有不同程度的抑制作用,其中Ｃｕ２＋和Ｈｇ２＋对该酶的抑制作用最强,其抑制机理均表现为非竞争性抑制     

黑曲霉单宁酶的纯化及部分性质研究

黑曲霉在五倍子单宁的诱导下产生单宁酶。通过（ＮＨ４）２ＳＯ４分形沉淀,ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ离子交换层析,Ｓｅｐｈａｄｅｘ Ｇ－１５０凝胶过滤,从黑曲霉的发酵液和菌丝中纯化得到凝胶电泳均一的单宁酶。酶作用的最适温度为３０℃,最适ｐＨ值为５．５,在温度１０－３０℃和ｐＨ４－６之间保持稳定,金属离子对单宁酶没有的激活作用,Ｆｅ＾２＋和Ｍｎ＾２＋则对酶有抑制作用。     

南欧紫荆（Cercis Siliquastrum L）种子中甘露聚糖酶的分离,纯化及…

南欧紫荆萌发种子的提取液，经硫酸铵分级沉淀，ＤＥＡＥ－纤维素柱层析，Ｂｉｏ－Ｇｅｌ Ｐ－６０和Ｓｅｐｈａｄｅｘ Ｇ－１００柱层析，得到了酶活为９８．６μ／ｍｇ的甘露聚糖酶。柱层析和聚丙烯酰胺凝胶电泳均表明其为均一性物质。南欧紫荆甘露聚糖酶由两个分子量为２万的相同亚基组成，可将以甘露聚糖为主链的多种半纤维素水解为含４￣６个单糖残基的寡糖片断。它的最适ｐＨ为５，最适温度为３７℃。Ｚｎ＾２＋对该酶活性有     

木霉4131菌株纤维素酶的分离纯化和部分性质研究

绿色木霉（Ｔｒｉｃｈｏｄｅｒｍａ Ｖｉｒｉｄｅ）突变菌株４１３１＾［１］产生的纤维素酶经过ｓｅｐｈａｄｅｘＧ－７５和ＤＥＡＥ－ｓｅｐｈａｄｅｘ－Ａ－２５两种层析分离,分离得到具有内切α－葡聚糖酶（ＣＭＣａｓｅ）活性的纯组分,提纯后酶的比活力提高到原来的２６．２倍;回收率为３２．８％,分子量为５６９００,等电点为４．０,最适反应温度为５５℃,最适ｐＨ为４．５,金属离子Ｋ＾＋,Ｎａ＾＋,Ｍｇ＾２＋对其     

绿色乳杆菌α－羟基戊二酸脱氢酶的纯化和特性研究

本文对绿色乳杆菌（Ｌ．Ｖｉｒｉｄｅｓｃｅｎｓ）Ｂ１７５产生的α－羟基戊二酸脱氢酶用硫酸铵盐析；ＤＥＡＥ—纤维素柱层折；超滤浓缩；苯基琼脂糖凝胶（ＰｈｅｎｙｌＳｅｐｈａｒｏｓｅ）疏水层析等方法纯化，使酶的纯度提高１１７倍。通过ＨＰＬＣ和等电聚焦测得该酶的分子量约为６６，０００，等电点为４．２。酶催化作用的最适ｐＨ５．５，最适温度为６０℃，对α—酮戊二酸和ＮＡＤＨ的Ｋｍ值分别为０．１４×１０（－３）Ｍ和０．０３×１０（－３）Ｍ。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.