HPLC示差折光分析法测定D-氨基葡萄糖盐酸盐中的N-乙酰-D-氨基葡萄糖

利用高效液相示差折光分析法测定D-氨基葡萄糖盐酸盐中的N-乙酰-D-氨基葡萄糖.方法采用Alltech C18柱(250×4.6 mm,5 μm),流动相为乙腈-醋酸盐缓冲液(3∶97),流速1 mL/min,柱温40℃,进样量20 μL.N-乙酰-D-氨基葡萄糖在4.18～1 079.33 μg/mL范~围内星显著的线性关系,相关系数为0.999 3.方法的加标回收率98.78%,检出限4.18 μg/mL.     

高效液相色谱-核粒子计数检测氨基葡萄糖类化合物

核粒子计数检测器是基于水凝聚激光计数专利技术(WCPC),对低紫外吸收的化合物有较强的优势.建立了高效液相色谱-核粒子计数检测器(HPLC-NQAD)检测D-氨基葡萄糖盐酸盐和N-乙酰-D-氨基葡萄糖的方法.采用AsahipakNH2(250mm×4.6mm,5μm)色谱柱,V(乙腈)∶V(0.5%三乙胺水溶液)=75∶25为流动相,流速为0.8mL/min,进样量为10μL,10min内完成检测.在NQAD蒸发管温度55℃、气体压力200kPa条件下,D-氨基葡萄糖盐酸盐与N-乙酰-D-氨基葡萄糖之间的分离度2.32,前者的检出限为16ng,定量限为50ng,后者的检出限为8ng,定量限为24ng.D-氨基葡萄糖盐酸盐在10.03～2 005.20μg/mL范围内线性回归相关系数为0.999 5,N-乙酰-D-氨基葡萄糖在10.18～2 036.80μg/mL范围内线性回归相关系数为0.999 9.检测D-氨基葡萄糖盐酸盐和N-乙酰-D-氨基葡萄糖的精密度实验的相对标准偏差(RSD)分别为1.53%和1.38%,平均回收率分别为100.18%和101.02%.该方法用于D-氨基葡萄糖盐酸盐和N-乙酰-D-氨基葡萄糖的检测,可靠、快速、简便.     

澳洲茄胺盐酸盐的质量控制

研究以高效液相色谱法控制澳洲茄胺盐酸盐质量的方法. 色谱柱为Agilent ZORBAXEclipse XDB-C₁₈柱(150 mm×4.6 mm, 5 μm), 流动相为V_{甲醇∶V乙腈∶V0.020 mol·L^-1乙酸铵=60 ∶35 ∶5, 流速为1 mL/min, 检测波长为205 nm, 进样量为10 μL. 结果表明, 采用该方法 澳洲茄胺盐酸盐与其他杂质分离良好, 线性 范围5~500 μg/mL(r=0.999 3, 理论塔板数n＞12 000, R＞1.5), 加 样平均回收率为98.6%(n=9), 重现性试验相对标准偏差（RSD）为0.618%.}     

反相高效液相色谱法测定氨基葡萄糖硫酸盐

改进了对硫酸氨基葡萄糖检测的反相高效液相色谱方法.柱前用异硫氰酸苯酯(GITC)衍生,在254 nm紫外波长处紫外检测洗脱液.色谱柱使用C18柱,流动相是由MeOH/H2O/CH3COOH(体积比为20∶79.96∶0.04)组成,流速为0.6 mL/min.结果显示,在氨基葡萄糖含量为8～100 μg/mL范围内呈线性关系(r=0.998 5),样品平均回收率为98.72%,RRSD=0.473% (n=5) .该方法简便、准确,可用于氨基葡萄糖硫酸盐的检测.     

RP-HPLC直接测定水果中维生素C含量

应用反相高效液相色谱法对时令水果中维生素C含量进行直接测定.方法: 色谱柱Eclipse XDB-C18 (5 μm 4.6×250 mm);流动相乙腈-水(40∶ 60 v/v);流速1.0 mL/min;检测波长275 nm.实验结果表明维生素C在(10 ～ 640) μg/mL范围内呈良好线性,R2=0.999 96,回收率为99.3% ～ 104.5%,RSD=1.65%.本法操作简便,结果准确、重现性好,为测定水果中维生素C的含量提供了一种有效方法.     

高效液相色谱法测定人血浆中硫普罗宁的浓度

建立了以反相高效液相色谱法测定人血浆中硫普罗宁浓度的方法.采用Agilent C8 (250 mm×4.6 mm,5μm)色谱柱,乙腈:水(用磷酸调pH至2.5) =8:92(v/v),为流动相,检测波长203 nm,流速1.0 mL·min-1.硫普罗宁浓度在1.5～1 600 μg·mL-1浓度范围内,峰面积与浓度...     

硫酸软骨素片含盐酸氨基葡萄糖量的测定

建立硫酸软骨素片含盐酸氨基葡萄糖量测定方法.采用Hypersil C18柱(4.6mm×250mm,5μm),以0.68％醋酸钠缓冲溶液-甲醇(80∶20)为流动相,检测波长340nm.盐酸氨基葡萄糖在100～2 000μg/mL的质量浓度范围内呈良好的线性关系(r=0.999 9),平均回收率为103.2％(RSD=...     

HPLC法同时测定石韦药材中绿原酸、芒果苷和木犀草素的质量浓度

建立了一种反相高效液相色谱法,同时分离石韦药材中绿原酸、芒果苷和木犀草素,并测定其质量浓度.采用超声优化提取石韦的有效成分:Phenomenex Gemini 5 μm C18(150×4.6 mm,5 μm)色谱柱,甲醇-冰乙酸溶液为流动相,梯度洗脱,流速为0.8 mL/min;并用波长程序进行检测,柱温35℃,外标法定量.结果表明,绿原酸、芒果苷、木犀草素的线性范围分别为0.084～40.00,0.084～40.00,0.084～13.33 μg/mL(R＞0.999),平均回收率为96.3%～97.8%,相对标准偏差小于2.16%.     

HPLC-ELSD法测定盐酸氨基葡萄糖胶囊中盐酸氨基葡萄糖的含量

采用高效液相色谱-蒸发光散射检测法测定了盐酸氨基葡萄糖胶囊的含量.使用氨基色谱柱(250 mm×4.6 mm,5μm),流动相组成为乙腈:水=(7:3),流速为1.0 m L/min,在盐酸氨基葡萄糖检测含量浓度范围为0.2~1.2 mg/m L时与峰面积呈线性关系,R2为0.9999,平均加样回收率为99.86%,RSD为1.37%.该法简单,准确,无需衍生反应,精密度和重现性较好,是盐酸氨基葡萄糖胶囊含量测定的有效方法.     

水产品中氧氟沙星对映体拆分研究

采用CHIRALCEL OD-H(4.6 mm×250 mm,5.0 μm,Daicel)手性色谱柱拆分了氧氟沙星对映体,考察了流动相组成和流速等对手性分离的影响.确定了最佳的拆分条件:流动相为正己烷-乙醇(60:40,v/v),流速为0.6 mL/min,柱温为20℃,检测波长为294 nm.在此条件下氧氟沙星对映体的分离度为1.69.左氧氟沙星和右氧氟沙星的检出限分别为0.02 μg/g和0.03 μg/g,定量限分别为为0.04 μg/g和0.05 μg/g.左氧氟沙星平均回收率为83.46％,日间精密度和日内精密度的分别为0.34％和0.44％.右氧氟沙星平均回收率为87.32％.日间精密度和日内精密度分别为0.46％和0.69％.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to