| 高效液相色谱法测定人血浆中硫普罗宁的浓度 |
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| 引用本文： | 郝二军,刘玉侠,徐桂清,李伟,胡志国,渠桂荣.高效液相色谱法测定人血浆中硫普罗宁的浓度[J].河南师范大学学报(自然科学版),2011,39(4):102-104. |
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| 作者姓名： | 郝二军 刘玉侠 徐桂清 李伟 胡志国 渠桂荣 |
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| 作者单位： | 1. 河南师范大学化学与环境科学学院,河南新乡,453007
2. 河南师范大学校医院,河南新乡,453007 |
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| 基金项目： | 国家自然科学基金(20802016); 河南省科技攻关计划项目(82102330029); 河南师范大学新引进博、硕士科研启动支持课题资助项目(522038) |
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| 摘 要： | 建立了以反相高效液相色谱法测定人血浆中硫普罗宁浓度的方法.采用Agilent C8 (250 mm×4.6 mm,5μm)色谱柱,乙腈:水(用磷酸调pH至2.5) =8:92(v/v),为流动相,检测波长203 nm,流速1.0 mL·min-1.硫普罗宁浓度在1.5～1 600 μg·mL-1浓度范围内,峰面积与浓度...
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| 关 键 词： | 硫普罗宁 高效液相色谱 血药浓度 药动学 |
Determination of Plasma Tiopronin Concentration by HPLC |
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| HAO Er-juna,LIU Yu-xiab,XU Gui-qing,LI Weia,HU Zhi-guo,QU Gui-rong.Determination of Plasma Tiopronin Concentration by HPLC[J].Journal of Henan Normal University(Natural Science),2011,39(4):102-104. |
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| Authors: | HAO Er-juna LIU Yu-xiab XU Gui-qing LI Weia HU Zhi-guo QU Gui-rong |
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| Institution: | HAO Er-juna,LIU Yu-xiab,XU Gui-qinga,LI Weia,HU Zhi-guoa,QU Gui-ronga(a.College of Chemistry and Environmental Science,b.Hospital of School,Henan Normal University,Xinxiang 453007,China) |
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| Abstract: | A HPLC method is established to determine the concentration of tiopronin in human plasma.The sample separation is performed on a Agilent C8 column(250 mm×4.6 mm,5 μm).The mobile phase is acetontrile―water(pH2.5 by phosphate)(8∶92,v/v)wit h a flow rate of 1.0 mL·min-1 and a detection wavelength of 203 nm.The linear range of tiopronin is 1.5～1 600 μg ·mL-1,(r=0.999 8).The extraction recovery of tiopronin is 74.26%～76.35% and the methodological recovery of it is 101.95%～106.86%.The intra-day RSD and inter-day RSD are 2.83%～3.75% and 5.92%～6.85%,respectively.This method is simple,rapid,accurate and reproducible,and it is applicable for the pharmacokinetic study of tiopronin in human plasma1. |
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| Keywords: | tiopronin HPLC plasma drug concentration pharmacokinetics |
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