极端嗜热厌氧纤维素分解菌基因文库的构建及其内切葡聚糖酶基因片段的克隆

以从云南邦拿掌温泉中分离、纯化的高温厌氧纤维素分解菌邦2菌(Cadicellulosiruptor)为材料,制备其总DNA,经限制性核酸内切酶EcoRⅠ部分酶切后,在T4DNA连接酶的作用下与经EcoRⅠ完全酶切、去磷酸化的质粒载体pUC18连接,然后转化E.coliJM109,建立了邦2的基因文库,经筛选鉴定得到6.3×103个重组子;重组子经刚果红平板验证:约有23.5%菌落呈现透明圈;重组子经EcoRⅠ酶切验证显示:重组质粒均含有外源DNA插入片段.结果表明已克隆到邦2菌纤维素酶系中的内切葡聚糖酶基因(ED基因)片段.     

止消灵软膏药效学实验研究

目的 探讨止消灵软膏对皮肤溃疡、烧烫伤的治疗效果.方法 Wistar大白鼠深Ⅱ度烫伤动物模型实验,共40只,体质量(250±50)g,雌雄各半,随机分成5组,每组8只.80％止消灵软膏实验组,50％止消灵软膏实验组,25％止消灵软膏实验组,磺胺嘧啶银组,凡士林空白对照组.结果 80％止消灵软膏组,50％止消灵软膏组,25％止消灵软膏组:20d后多数创面基本愈合.磺胺嘧啶银组:创面情况略大于前2组,而小于25％止消灵软膏组.凡士林组:20d后仍未愈合.结论 止消灵软膏与磺胺嘧啶银治疗烧烫伤疗效显著,80％止消灵软膏、50％止消灵软膏的疗效与磺胺嘧啶银相似,前3组与25％止消灵软膏疗效有差异.     

实验性糖尿病动物模型制备及指标测定评价

目的：综合实验性糖尿病的动物模型及指标测定的特征，以评价他们的优缺点。方法：对国内外文献进行归纳、分析及总结。结果：系统阐述该领域在国内外的研究现状，为今后的研究起到指导作用。结论：实验性糖尿病的动物模型及测定的指标很多，各有其特点。     

用CTAB+磁珠法提取火烧骨DNA的研究

对不同焚烧程度的长骨用CTAB法提取DNA和用DNAIQ^TMSystem试剂盒纯化方法，较有效解决火烧骨的DNA的提取和荧光STR复合扩增检验的问题，为今后对杀人焚尸、火灾、爆炸等恶性案件和事故中进行个人识别和亲子鉴定提供了一定的科学依据。     

Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

A surface-based DNA algorithm for the minimal vertex cover problem

Abstract DNA computing was proposed for solving a class of intractable computational problems, of which the computing timewill grow exponentially with the problem size. Up to now, many achievements have been made to improve its performance and increase itsreliability. It has been shown many times that the surface-based DNA computing technique has very low error rate, but the technique hasnot been widely used in the DNA computing algorithms design. In this paper, a surface-based DNA computing algorithm for minimal ver-tex cover problem, a problem well-known for its exponential difficulty, is introduced. This work provides further evidence for the abilityof surface-based DNA computing in solving NP-complete problems.     

生物机制在智能计算中的应用

揭示生物机制在智能计算中的应用。与生物学相关理论相结合 ,论述遗传算法、人工神经网络和DNA计算的生物机制 ,揭示生物学思想在其中的应用 ,说明现在各类学科交叉发展的趋势     

Genetic Diversity in Populations of Sepiella maindroni Using 16S rRNA Gene Sequence Analysis

Part of the 16S rRNA gene is amplified with PCR and sequenced for 5 populations of common Chinese cuttlefish Sepiella maindroni:three from the South China Sea,one from East China Sea and one from Japan.The result shows that a total of 5 nucleotide positions are found to have gaps or insertions of base pairs among these individuals,and 13 positions are examined to be variable in all the sequences,which range from 494 to 509 base pairs.All of the individuals are grouped into 7 haplotypes (h1-h7).No marked genetic difference is osberved among those populations.All of the individuals from Nagasaki belong to h1 and the h3 haplotype is found only in the coastal waters of China.A→←G transition in Nucleotide 255 is suggested to be taken as a kind of genetic marker to identify the populations distributed in East-South China Sea and the Nagasaki waters of Japan.     

彗星电泳检测细胞DNA损伤应用新进展

彗星电泳是近年发展起来的一种测定单个细胞DNA损伤的方法 ,因其操作简单、快速及灵敏等特点 ,应用范围十分广泛 .着重介绍了这一技术的发展、图形分析及应用进展     

基因工程中常用的酶及其功能分析

酶是活细胞所产生的一种具有特殊催化功能的蛋白质,在生物工程中应用非常广泛,如食品、医药、废水处理等方面.基因工程中常用的酶主要包括核酸酶、连接酶、聚合酶和修饰酶,分别发挥着不同的功能.