| Real time observation of the photocleavage of single DNA molecules |
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| 作者姓名： | LIBin HUJun WANGYing WUShiying HUANGYibo LIMinqian |
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| 作者单位： | ShanghaiInstituteofNuclearResearch,ChineseAcademyofSciences,Shanghai201800,China |
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| 基金项目： | 国家自然科学基金，中国科学院资助项目，上海市科委资助项目 |
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| 摘 要： | A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.
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| 关 键 词： | 单个DNA分子 光致断裂 原子力显微镜 荧光显微镜 实时观察 生物大分子 |
| 收稿时间： | 2002-10-02 |
| 修稿时间： | 2002-12-23 |
Real time observation of the photocleavage of single DNA molecules |
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| LIBin HUJun WANGYing WUShiying HUANGYibo LIMinqian.Real time observation of the photocleavage of single DNA molecules[J].Chinese Science Bulletin,2003,48(7):673-675. |
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| Authors: | Bin Li Jun Hu Ying Wang Shiying Wu Yibo Huang Minqian Li |
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| Institution: | LI Bin , HU Jun , WANG Ying, WU Shiying, HUANG Yibo & LI Minqian Shanghai Institute of Nuclear Research,Chinese Academy of Sciences, Shanghai 201800,China |
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| Abstract: | A method for real time observation of photo-cleavage of stretched λDNA at single molecular level by afluorescent microscope coupled with CCD is developed. DNAmolecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the process of photocleavage and relaxation of DNAunder radiation of blue light is observed. We speculate thatthe conformation change of stretched DNA and the effect ofwater are likely to facilitate the effect of YOYO photocleav-age DNA molecules. The photocleavage effect of YOYO forstretched DNA may be useful to study DNA elasticity, cancerresearch as well as the interaction between DNA and dyes. |
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| Keywords: | photocleavage DNA single molecule atomic force mi-croscope (AFM) substrate modification fluorescent micro-scope(FM) |
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