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1.
用组织培养的方法,探讨了桑树萎缩病茎段再健康植株的条件,结果显示,严重呈现萎缩症状的桑树茎段在不含任何植物激素的MS培养基上生长1~3个月,形成幼苗盆栽后2年中不再显现任何症状,用4,6-二脒基-2-苯基吲哚盐酸盐(DAPI)检测表明组培植株中不于含有类菌原体。  相似文献   

2.
通过聚合酶链式反应(PCR),用多对引物探讨了桑树萎缩病类菌原体的分子检测.结果显示,所用引物中只有R16F2/R2和R16(Ⅰ)F1/R1适用于桑树萎缩病类菌原体的检测,呈现不同症状的黄化型、萎缩型和其它两种类型的病株都扩增出类菌原体16SrDNA的片段.根据组特异性引物对四种症状类型中类菌原体16SrDNA的扩增,对我国桑树萎缩病类菌原体的分组状况进行了讨论.  相似文献   

3.
耐盐辣椒下胚轴和茎段离体培养与植株再生研究初报   总被引:4,自引:0,他引:4  
报道了耐盐辣椒下胚轴和茎段离体培养的初步研究结果.1.耐盐辣椒的种子在无菌条件下发芽缓慢,萌发率低,至接种后20d时,萌发率仍不足50%;2.约有26.7%的下胚轴外植体在附加5.0mg/L6-BA的MS培养基上可形成丛芽;3.被分割开的丛芽或带一叶一腋芽的茎节在附加0.5mg/LIBA的MS培养基上可形成具发达根系的完整植株,将试管苗切割成带一叶一腋芽的茎段,用该培养基反复继代培养以达到快速离体繁殖的目的;4.试管苗的移栽成活率可达90%以上  相似文献   

4.
毛花猕猴桃愈伤组织诱导与植株再生   总被引:8,自引:0,他引:8       下载免费PDF全文
从毛花猕猴桃(ActinidiaerianthaBenth)田间生长的茎段、种子无菌条件下萌发的下胚轴及试管苗的茎段和叶片得到愈伤组织。田间来源的茎段诱导愈伤组织较难,愈伤组织生长缓慢;下胚轴及试管苗茎段和叶片容易产生愈伤组织,愈伤组织生长旺盛。消毒时间、接种方法、基因型和培养基都可能影响到田间来源的茎段愈伤组织产生。在MS附加玉米素或6-苄氨基嘌呤(BAP)的培养基上下胚轴来源的愈伤组织不经转代即分化芽和根。试管苗茎段和叶片在附加玉米素或N-(2-氯基-4-吡啶基)-N′-苯基脲(CPPU)的MS培养基上培养一代也分化出芽、试管苗茎段在附加0.0025mg/LCPPU和0.1mg/L吲跺乙酸(IAA)的MS培养基上产生愈伤组织、芽的分化和苗生长都较理想;试管苗叶片则以附加0.025mg/LCPPU和0.1mg/LIAA或0.5mg/L玉米素和0.1mg/LIAA的MS培养基较好。当苗伸长至1cm或以上时切下,转入MS基本培养基(大量元素减半)上后可形成完整植株。  相似文献   

5.
四棱豆茎段离体培养的研究   总被引:1,自引:0,他引:1  
通过茎段离体培养,实现了四棱豆的快速无性繁殖.结果表明:(1)用浓H2SO4将其种子处理8min后再消毒接种可获得最高的发芽率;(2)在附加1.0mg/L6-BA的H培养基上,每粒种子平均可形成13个丛芽;(3)在增殖培养阶段,IBA浓度为1.0~2.0mg/L时对促进四棱豆离体茎段生根成苗的效果最为理想;(4)增殖培养阶段以采用H培养基的效果最为理想;(5)单独使用IBA时,即使其浓度高达4.0mg/L也只诱导离体茎段生根,绝大多数外植体不会产生愈伤组织,只有极少数外植体产生少量愈伤组织;低浓度的6-BA(0.5mg/L)或高浓度的6-BA(2.0mg/L)配以低浓度的IBA(0.25mg/L)反而会引起大多数离体茎段的基部产生大量愈伤组织.  相似文献   

6.
首次合成一种链式苯并酰胺配体N,N'-双(2-氨基苯甲酰基)-1,3-丙二胺.用元素分析,红外,紫外-可见光,核磁共振等对配体进行表征和确证.用pH-电位法测定了配体的加质子常数βH1及其4种过渡金属离子所形成的去质子MH-2L型配合物的稳定常数K.结果,βH1=2.42×102,βH2=4.15×103;logK分别为:-9.83(Cu2+);-13.76(Ni2+);-14.13(Co2+);-12.12(Zn2+).配位情况类似于低肽的配合物,且形成1∶1的中性分子,稳定性顺序Co2+<Ni2+<Cu2+>Zn2+,和Lrving-Wiliams顺序一致.  相似文献   

7.
用聚乙二醇-400作为相转移催化剂合成2-(三氯甲基)-2-丙醇,反应在碱性介质中进行,条件温和,反应温度控制在5~10℃原料的摩尔配比为丙酮:氯仿:氢氧化钠:聚乙二醇-400=10:10:7:1.产率为30.4%。  相似文献   

8.
用3-硝基-1,2,4-三唑-5-酮(NTO)的锂盐水溶液与Eu2O3的稀硝酸溶液反应,合成了NTO的铕配合物,其单晶结构用X射线分析法进行了测定,确定了它的结构式为〔Eu(NTO)3(H2O)5〕5H2O。其晶体属单斜晶系,空间群为P21/n,最终偏离因子R为0.023。晶胞参数如下:a1.8720(2)nm,b0.6548(3),c1.9323(3)nm,β95.33(1)°,V2.3583(5)nm3,Z4,Dc2.026gcm-3,μ27.678cm-1,F(000)1432。铕离子的配位数为8,其配位多面体为畸变的十二面体  相似文献   

9.
在2,4-D诱导下,接种豇豆根瘤菌,能诱导青稞根部结瘤,在其根瘤中有一定数量的接种根瘤菌;同时,2,4-D诱导结瘤的青稞植株的株高、株重均比对照高,而根长、叶片数、绿叶数比对照低,其根系生长受到严重抑制.  相似文献   

10.
用β-氨基-α-羟已基二茂铁与二价钴、镍、铜、锌、镉和汞的氯化物及醋酸铜在乙醇-水中反应,合成了七种新的配合物.探索出了适宜的反应条件,经元素分析,确定了新配合物的组成为:L2MX2·nH2O(L=Fc─CHCH2NH2;M=Co2+、Ni2+、Cu2+、Zn2+、Cd2+、Hg2+,x=Cl-,CH3COO-;n=0,2,4).通过红外光谱和核磁共振氢谱.对这些配合物的结构进行了表征.  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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