四棱豆茎段离体培养的研究

通过茎段离体培养，实现了四棱豆的快速无性繁殖．结果表明：（１）用浓Ｈ２ＳＯ４将其种子处理８ｍｉｎ后再消毒接种可获得最高的发芽率；（２）在附加１．０ｍｇ／Ｌ６－ＢＡ的Ｈ培养基上，每粒种子平均可形成１３个丛芽；（３）在增殖培养阶段，ＩＢＡ浓度为１．０～２．０ｍｇ／Ｌ时对促进四棱豆离体茎段生根成苗的效果最为理想；（４）增殖培养阶段以采用Ｈ培养基的效果最为理想；（５）单独使用ＩＢＡ时，即使其浓度高达４．０ｍｇ／Ｌ也只诱导离体茎段生根，绝大多数外植体不会产生愈伤组织，只有极少数外植体产生少量愈伤组织；低浓度的６－ＢＡ（０．５ｍｇ／Ｌ）或高浓度的６－ＢＡ（２．０ｍｇ／Ｌ）配以低浓度的ＩＢＡ（０．２５ｍｇ／Ｌ）反而会引起大多数离体茎段的基部产生大量愈伤组织．

Study on the Stem Segment Culture of Indies Goabean ( Psophocarpus tetragonolobus DC.) in Vitro

This paper deals with the in vitro culture of the stem segment of Indies Goabean( Psophocarpus tetragonolobus DC.). The experimental results are as follows: (1)The highest germination rate could be obtained in the treatment that the seed was treated for 8 min with 98% sulfuric acid before sterilization and inoculation; (2) 13 clustered shoots per seed were formed on the H medium complemented with 1.0 mg/L 6-BA,averagely; (3) The effect on the rootage and germination of the axillary buds of the in vitro stem segements was the most ideal if the concentration of IBA ranges from 1.0 mg/L to 2.0 mg/L; (4) Among the four basic media tested in the multiplation culture, H medium was the most ideal; (5) If IBA is merely complemented, the rootage of the in vitro stem segments could only be induced, the callus could not be formed in the most explants, and only a few calli were formed in a few explants even the concentration of IBA was as high as 4.0 mg/L. Contrarily, a low concentration of 6-BA(0.5 mg/L), or a high concentration of 6-BA(2.0 mg/L) matching with a low concentration of IBA(0.25 mg/L) could result in the formation of lots calli in the most of the in vitro stem segments.