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1.
库拉索芦荟组织培养研究   总被引:4,自引:0,他引:4  
以库拉索芦荟为材料进行组织培养研究,分别以根、茎节、茎尖和叶为外植体接种于不同的诱导培养基中诱导芽的分化,待芽长至5cm左右地将其接入不同的生根培养基中生根,结果表明:茎节接种于MS(1962)+6-BA4.0mg/L+NAA0.2mg/L诱导效率最高,而根没有产生不定芽;1/2MA+IAA0.25mg/L+NAA0.25mg/L+活性炭0.3g/L有利于试管苗的生根。  相似文献   

2.
辣椒(Capsicum annuum L.)下胚轴离体培养的研究   总被引:4,自引:0,他引:4  
将辣椒无菌苗下胚轴切段接种在MS培养基(附加不同的植物激素)上,诱导产生愈伤组织。最适的诱导培养基为MS+2mg/L BA,0.5mg/L IAA,在此培养基上产生的愈伤组织具有较强的分化能力。将愈伤组织转移到分化培养基,光照培养,芽分化率可达25.0%。不定芽在含1mg/L GA3和2mg/L BA的MS培养基上伸长成苗,再移入生根培养基上诱导生根,长成完整植株。  相似文献   

3.
海南中草药材鸡屎藤离体培养的研究   总被引:5,自引:0,他引:5  
利用组织培养方法对鸡屎藤进行离体培养的研究,结果表明:以鸡屎藤茎节、嫩叶作外植体能诱导产生愈伤组织,并能诱导产生不定芽.诱导愈伤组织的培养基以 MS+6-BA 0.5mg·L-1+NAA0.5mg·L-1效果较好;诱导不定芽的培养基以MS+6-BA4mg·L-1+NAA0.5mg·L-1效果较好;本文还报道了鸡屎藤的生根和移栽技术.  相似文献   

4.
毛花猕猴桃愈伤组织诱导与植株再生   总被引:8,自引:0,他引:8       下载免费PDF全文
从毛花猕猴桃(ActinidiaerianthaBenth)田间生长的茎段、种子无菌条件下萌发的下胚轴及试管苗的茎段和叶片得到愈伤组织。田间来源的茎段诱导愈伤组织较难,愈伤组织生长缓慢;下胚轴及试管苗茎段和叶片容易产生愈伤组织,愈伤组织生长旺盛。消毒时间、接种方法、基因型和培养基都可能影响到田间来源的茎段愈伤组织产生。在MS附加玉米素或6-苄氨基嘌呤(BAP)的培养基上下胚轴来源的愈伤组织不经转代即分化芽和根。试管苗茎段和叶片在附加玉米素或N-(2-氯基-4-吡啶基)-N′-苯基脲(CPPU)的MS培养基上培养一代也分化出芽、试管苗茎段在附加0.0025mg/LCPPU和0.1mg/L吲跺乙酸(IAA)的MS培养基上产生愈伤组织、芽的分化和苗生长都较理想;试管苗叶片则以附加0.025mg/LCPPU和0.1mg/LIAA或0.5mg/L玉米素和0.1mg/LIAA的MS培养基较好。当苗伸长至1cm或以上时切下,转入MS基本培养基(大量元素减半)上后可形成完整植株。  相似文献   

5.
播娘蒿子叶及下胚轴诱导再生植珠的研究   总被引:1,自引:0,他引:1  
用播娘蒿种子,在附加6-BA0.5~1.0mg/L+NAA0.1mg/L的MS培养基上获得无菌苗,幼苗的下胚轴在含6-BA0.1mg/L+NAA0.5mg/L的MS培养基上出愈情况最佳,将来自下胚轴的愈伤组织培养于含有6-BA2.0mg/L+NAA0.2mg/L的MS培养基上分化出的丛生芽状态最好,最佳生根培养基为1/2MS+IBA0.5mg/L+NAA0.05mg/L.  相似文献   

6.
玛丽安万年青的茎尖培养与快速繁殖   总被引:3,自引:0,他引:3  
以玛丽这万年青(Dieffenbachis Tropic Marinne)的顶芽或侧芽茎尖作外植体,通过二次表面灭菌,得到茎尖无菌培养物。根据茎尖愈伤组织诱导率和生长速度2项指标,筛选出码丽安万年青茎尖离体培养的培养基。MS+BA4.0ng/L的固体培养基适宜于诱导茎尖形成愈伤组织和愈伤组织生长。愈伤组织转入MS+BA2.0mg/L的固体培养基分化形成丛生芽,从愈伤组织上切取1cm以上的牙转入MS  相似文献   

7.
无花果的组织培养   总被引:7,自引:0,他引:7  
对影响无花果外植体不定芽诱导的四个因子进行了研究:不同器官、培养基的组成、外植体的预处理和不同的品种。细胞分裂素BA的效果优于KT。茎尖和茎段不定芽诱导培养基为mg·L ̄(-1)(MS+BA),叶片的分化以浓度8mg·L ̄(-1)的BA效果最好。赤霉素(GA_3)有助于芽的萌发和生长,浓度在0.5~1mg·L ̄(-1)为宜。割取嫩枝插入1/2MS,附加1BA或NAA1.0~2.0mg·L ̄(-1)的生根培养基中,根原基诱导率95%以上,移栽成活率90%以上。  相似文献   

8.
荞麦组织培养中的植株再生   总被引:1,自引:0,他引:1  
用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株。实验结果表明,适宜诱导愈伤组织的激素组合为2.4-D4.0mg/L+KT0.2mg/L,适宜愈伤组织生长的激素组合为2.4-D1.0mg/L+KT1.0mg/L。诱导芽的较适宜的组合为6-BA3.0mg/L+IAA0.1mg/L,不定芽接种到含MS+IBA2.0mg/L+KT0.1mg/L的MS培养基上就能生  相似文献   

9.
百合基因转化受体系统的建立   总被引:2,自引:0,他引:2  
以百合叶片为外植体不经愈伤组织直接诱导并且发育成带球径的大苗进行了研究,结果表明:叶片分芽培养基为MS+BA0.5mg/L+ZT0.1mg/L+NAA1.0mg/L,生根培养基为1/2MS+NAA0.2~1.0mg/L,成球并且生长培养基为MS+IAA1.0mg/L,移栽成活率为100%.从而建立了百合基因转化的受体系统  相似文献   

10.
绿巨人快速繁殖的研究   总被引:2,自引:1,他引:1  
以观赏植物绿巨人的茎尖为外植体,采用不同的培养基配方,研究绿巨人的快速繁殖技术.结果表明:MS+6-BA(3.0 mg·L-1)+NAA(0.5 mg·L-1)对诱导愈伤组织和促进芽的分化效果最好,芽分化数最多;不同浓度的LaCl3可提高芽的增殖率,并促进芽生长;而MS+NAA(0.5mg·L-1)+AC(0.2 mg·L-1)则促进根的分化和生长.  相似文献   

11.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

12.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

13.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

14.
Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to  相似文献   

15.
Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications (up to 574.8 km/ h test speeds or 380 km/h commercial speeds) in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.  相似文献   

16.
17.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

18.
Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.  相似文献   

19.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

20.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

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