| 玛丽安万年青的茎尖培养与快速繁殖 |
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| 引用本文： | 张苏锋,赵兴兵,赵冬梅.玛丽安万年青的茎尖培养与快速繁殖[J].信阳师范学院学报(自然科学版),2000,13(1):95-98. |
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| 作者姓名： | 张苏锋 赵兴兵 赵冬梅 |
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| 作者单位： | 1. 信阳师范学院生物系,河南信阳,464000
2. 通许一中,河南通许,452270 |
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| 基金项目： | 河南省教委科技攻关资助项目(97210036;98210039);河南省科委利技攻关资助项目(981050018) |
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| 摘 要： | 以玛丽这万年青（Ｄｉｅｆｆｅｎｂａｃｈｉｓ Ｔｒｏｐｉｃ Ｍａｒｉｎｎｅ）的顶芽或侧芽茎尖作外植体，通过二次表面灭菌，得到茎尖无菌培养物。根据茎尖愈伤组织诱导率和生长速度２项指标，筛选出码丽安万年青茎尖离体培养的培养基。ＭＳ＋ＢＡ４．０ｎｇ／Ｌ的固体培养基适宜于诱导茎尖形成愈伤组织和愈伤组织生长。愈伤组织转入ＭＳ＋ＢＡ２．０ｍｇ／Ｌ的固体培养基分化形成丛生芽，从愈伤组织上切取１ｃｍ以上的牙转入ＭＳ
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| 关 键 词： | 玛丽安万年青 茎尖培养 快速繁殖 组织培养 |
| 文章编号： | 1003-0972(2000)01-0095-04 |
Shoot apex culture and rapid propagation of Dieffenbachia Tropic Marianne |
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| ZHANG Su feng ,ZHAO Xing bing ,ZHAO Dong mei.Shoot apex culture and rapid propagation of Dieffenbachia Tropic Marianne[J].Journal of Xinyang Teachers College(Natural Science Edition),2000,13(1):95-98. |
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| Authors: | ZHANG Su feng ZHAO Xing bing ZHAO Dong mei |
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| Institution: | ZHANG Su feng 1,ZHAO Xing bing 1,ZHAO Dong mei 2 |
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| Abstract: | The explant originated from shoot apex of terminal bud or lateral bud of Dieffenbachia Tropic Marianne was throughly surface sterilized by double sterilizing.According to the frequency of induction and growth speed of callus,which were used in this experiments,the optinum medium for cultured shoot apex in vitro was MS mediums with BA 4.0 mg/L.The callus differentiated into plenty of buds on an agar-solidified MS mediums containing BA 2.0mg/L.Shoots cut from callus were rooted in an agar-solidified MS mediums containing NAA 1.0mg/L.Plantlets were transplanted after rooting culture for 50 days.The survival rate of transplanted plantlet was 95%. |
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| Keywords: | Dieffenbachia Tropic Marianne shoot apex culture rapid propagation |
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