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1.
以甜樱桃矮化砧木Gisela5和Gisela6作为试材,研究优化了其离体快繁的条件,结果表明:提高基本培养基中氮素含量可提高增殖系数,含有NH4NO33300mg/L和KNO33800mg/L的MS基本培养基中添加BA0.5mg/L和IBA0.1mg/L的培养基,为Gisela5和Gisela6的最适增殖培养基,其增殖系数可达7—8,在1/2MS IBA0.3mg/L的生根培养基上,生根率达89.3%,每个外植体可生出3—4条根,生根苗移栽容易成活。  相似文献   

2.
以甜樱桃矮化砧木Gisela5和Gisela6作为试材,研究优化了其离体快繁的条件.结果表明:提高基本培养基中氮素含量可提高增殖系数,含有NH4NO33300mg/L和KNO33800mg/L的MS基本培养基中添加BA0.5mg/L和IBA0.1mg/L的培养基,为Gisela5和Gisela6的最适增殖培养基,其增殖系数可达7-8.在1/2MS+IBA0.3mg/L的生根培养基上,生根率达89.3%,每个外植体可生出3-4条根.生根苗移栽容易成活.  相似文献   

3.
闵炜  陈志萍 《江西科学》2009,27(2):219-223
在试管中诱导金边瑞香芽苗生根,要同时添加NAA和IBA或NAA和IAA才能形成良好的根系,添加足够的蔗糖对提高生根率,增加每株生根数和根的长度都至关重要。通过对6种生根培养基比较,确定1/2MS+NAA0.5mg/L+IBA0.5mg/L+3%蔗糖为最佳的诱导生根培养基,平均生根率达84.6%。  相似文献   

4.
紫草的组织培养及快速繁殖研究   总被引:1,自引:1,他引:0  
以紫草的嫩茎为材料,进行了愈伤组织的诱导、愈伤组织的分化、不定芽的生根和生根继代、试管苗的移栽和移植的研究,建立起紫草嫩茎的无性系,达到了快速繁殖的目的.结果表明:MS+BA0.5mg/L+NAA1.8mg/L是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;MS+AgNO31.5mg/L+BA0.3mg/L+NAA0.1mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.4mg/L是试管苗生根培养的理想培养基;移植后试管苗保持了野生植株的各种生物性状,后期出现了生长旺盛、根系发达的特点.  相似文献   

5.
细叶百日草变异植株的组织培养及无性系建立   总被引:1,自引:0,他引:1  
以变异细叶百日草嫩茎为材料,进行了愈伤组织的诱导、分化、试管苗生根、移栽的研究.结果表明:MS+BA1.0+2,4-D1.2为嫩茎愈伤组织诱导培养和继代培养的理想培养基;MS+Ag(NO3)2 2.0+BA0.4是愈伤组织和不定芽分化培养的理想培养基;1/3MS+IAA0.5是生根培养的理想培养基;以炉灰渣为基质移栽的成活率为94%,扦插的成活率为89%;定植成活的试管苗保持了后期生长很旺盛变异性状,出现了根系增加1倍左右的特点.  相似文献   

6.
小根蒜组织培养及无性系建立的研究   总被引:1,自引:1,他引:0  
以小根蒜嫩茎的鳞茎为材料,进行了愈伤组织诱导、不定芽分化、试管苗生根、试管苗移栽和移植的研究,建立了小根蒜嫩茎无性系。结果证明:MS+6-BA0.3mg·L^-1+2,4-D1.0mg·L^-1+NAA0.5-1.0mg·L^-1是小根蒜愈伤组织的诱导培养和继代培养的理想培养基;Ms+AgNO31.2mg·L^-1+BA0.5mg·L^-1+NAA0.1mg·L^-1是愈伤组织和不定芽分化的理想培养基;1/3MS+NAA0.3-0.6mg·L^-1是变异小根蒜不定芽生根的理想培养基;炉灰渣是试管苗移栽的理想基质。  相似文献   

7.
湿地松丛生芽的诱导及试管苗的生根研究   总被引:5,自引:1,他引:4  
利用湿地松种子进行丛生芽的诱导及试管苗生根研究。结果发现:改良GD培养基适合湿地松离体胚的生长;以湿地松带子叶顶芽为外植体,在附加有3mg/L 6-BA和0.05mg/L的NAA改良GD培养基上可诱导湿地松丛生芽的形成,诱导率最高为73.3%;湿地松试管苗不定根诱导较为适宜的组合是1/2MS+0.05mg/L NAA,诱导率可达60%,而无激素的1/2改良GD有利于根的伸长。  相似文献   

8.
欧林达夏橙组织培养研究   总被引:3,自引:0,他引:3  
比较不同基本培养基、不同外植体、不同生长调节物质种类及配比对欧林达夏橙实生苗芽诱导及根分化的影响.结果表明,子叶节段诱导芽效果较佳,MS为基本培养基附加生长调节物质BA2mg/L,NAA0.2mg/L适于芽的诱导,White为基本培养基附加生长调节物质IBA2mg/L对根的诱导较佳,生根率达100%,移栽成活率达94%.  相似文献   

9.
穿龙薯蓣嫩茎高效无性系建立的研究   总被引:1,自引:0,他引:1  
研究了穿龙薯蓣嫩茎愈伤组织的诱导和分化、不定芽的分化和生根、试管苗移栽和扦插及移植于山坡栽培所需要的条件,建立起穿龙薯蓣嫩茎的高效无性系及快速繁殖技术。结果证明:MS+BA0.3mg/L+1NAA1.6mg/L是诱导嫩茎形成颗粒状愈伤组织的理想培养基;MS+AgNO32.0mg/L+BA1.0mg/L+NAA0.1mg/L是颗粒状愈伤组织和不定芽分化培养的理想培养基;1/2MS+NAA0.4mg/L是试管苗生根培养和生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。移植的试管苗具有长势旺盛、春天发芽早、根系发达等性状特点。  相似文献   

10.
新几内亚凤仙离体快速繁殖技术研究   总被引:3,自引:0,他引:3  
取新几内亚凤仙具有顶芽或腋芽的新生茎段为外植体,诱导芽体萌发,进行繁殖培养.通过大量试验,筛选出各阶段适宜的培养基组成.萌芽诱导阶段培养在MS 6-BA2.0mg/L NAA0.2mg/L中,顶芽萌发早,生长快;在继代和增殖培养中以MS 6-BA1.5mg/L NAA0.2mg/L培养基效果最好.1/2MS NAA0.2mg/L诱导生根,生根率可达100%.根质量较好;适宜条件下炼苗移栽.试管苗成活率缺95%以上。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

13.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

14.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

15.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

16.
Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.  相似文献   

17.
以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.  相似文献   

18.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

19.
Quality traits in wheat (Triticum aestirum L.) were studied by quantitative trait locus (QTL) analysis in a recombinant inbred line (RIL) population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross (ChSh). Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.  相似文献   

20.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

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