| 新几内亚凤仙离体快速繁殖技术研究 |
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| 引用本文： | 刘涛,陈伟,赵博生.新几内亚凤仙离体快速繁殖技术研究[J].山东理工大学学报,2003,17(1):103-106. |
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| 作者姓名： | 刘涛 陈伟 赵博生 |
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| 作者单位： | [1]山东理工大学生命科学学院，山东淄博255049 [2]淄博市开发区园林管理处，山东淄博255086 |
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| 摘 要： | 取新几内亚凤仙具有顶芽或腋芽的新生茎段为外植体，诱导芽体萌发，进行繁殖培养．通过大量试验，筛选出各阶段适宜的培养基组成．萌芽诱导阶段培养在MS 6-BA2．0mg／L NAA0．2mg／L中，顶芽萌发早，生长快；在继代和增殖培养中以MS 6-BA1．5mg／L NAA0．2mg／L培养基效果最好．1／2MS NAA0．2mg／L诱导生根，生根率可达100％．根质量较好；适宜条件下炼苗移栽．试管苗成活率缺95％以上。
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| 关 键 词： | 新几内亚凤仙 离体快速繁殖 组织培养 培养基 芽增殖 试管苗 |
| 文章编号： | 1007-1857(2003)01-0103-04 |
| 修稿时间： | 2002年12月10 |
Study on Rapid Propagation Techniques of Impatiens Hawkerii in Vitro Culture |
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| LIU Tao ,CHEN Wei ,ZHAO Bo sheng.Study on Rapid Propagation Techniques of Impatiens Hawkerii in Vitro Culture[J].Journal of Shandong University of Technology:Science and Technology,2003,17(1):103-106. |
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| Authors: | LIU Tao CHEN Wei ZHAO Bo sheng |
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| Institution: | LIU Tao 1,CHEN Wei 2,ZHAO Bo sheng 1 |
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| Abstract: | The new stem nodes of Impatiens hawkerii with terminal buds and axillary buds were taken as explant cultured in vitro to induce buds sprouting and multiplication. Media supplemented with different plant hormones and concentration of them were required for different stage of the culture. The medium for germination: MS+6 BA2.0mg/L+NAA0.2mg/L. Terminal buds of the explants sprouted early and grew fast in this medium. The medium for subculture and shoot multiplications: MS+6 BA1.5mg/L+NAA0.2mg/L, which was the first option. The medium for rooting:1/2MS+NAA0.2mg/L. The rooting rate was 100% and the quality of the roots grew well. The survival rate of the plantlet was 95% after transplanting under suitable condition. |
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| Keywords: | Impatiens hawkerii bud propagation plantlet |
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