健康人Ig/C3双特异性免疫复合物与体液免疫的相关性

采用直线相关分析法,较系统地研究了健康人Ｉｇ／Ｃ３双特异性循环免疫复合物（ＩｇＭ／Ｃ３－ＴＣＩＣ、ＩｇＧ／Ｃ３－ＴＣＩＣ和ＩｇＡ／Ｃ３－ＴＣＩＣ）与ＩｇＭ、ＩｇＧ、ＩｇＡ和Ｃ３的相关性,比较了Ｉｇ／Ｃ３－ＴＣＩＣ和Ｃ３／Ｉｇ－ＴＣＩＣ分别与有关体液免疫指标关系的异同。结果发现,ＩｇＭ／Ｃ３－ＴＣＩＣ与总ＩｇＭ和总ＩｇＡ／Ｃ３－ＴＣＩＣ与总ＩｇＭ、总ＩｇＭ、总ＩｇＧ、总ＩｇＡ、和复合ＩｇＧ以及总Ｃ３     

Construction of human combinatorial antibody library and screening of monoclonal antibody Fabs to human immunodeficiency virus type I

The heavy chain Fd genes and K chain genes of immunoglobulin were amplified by RT-PCR from PBL of three volunteer donors with HIV-positive. Phage antibody library was constructed with the Fd genes and K chain genes using pComb3 as vector. Three-round selection against coated gp120 showed specific enrichment of phage antibodies. After the third round selection, 40 out of 50 clones exhibited gp120 binding capacity. The specificity of the clones was verified by ELISA and competition inhibition ELISA. The VH was derived from subgroups VH Ⅱ and VHⅢ, VL belonged to subgroups VKⅠ and VK Ⅲ with DNA sequencing. These results suggest that the antibodies obtained are specific to gp120.     

仅有三个悬挂点的图的补图的最小特征值

设图G是点集为V(G)＝{v1,v2,…,vn}的简单连通图,则G的邻接矩阵是A(G)＝(aij)n×n,其中若vi和vj相邻,则aij＝1,否则aij＝0.由于A(G)是实对称的,因此可将其特征值设为λ1(G)≥λ2(G)≥…≥λn(G),且A(G)的特征值也称为G的特征值.该文在仅有三个悬挂点的图的所有连通补图中,确定了其最小特征值达到最小值时的唯一图.     

陈373块薄层稠油油藏N2和CO2补充地层能量可行性研究

为探索N2、CO2对稠油物性参数的影响规律,采用室内实验与数值模拟相结合的方法。实验测定注入N2、CO2后地层条件下稠油体积膨胀系数、黏度、溶解系数等物性参数,并利用CMG油藏数值模拟软件进行CO2、N2补充地层能量的数值模拟研究。研究结果表明,在一定的温度和所测压力范围内,CO2和N2在陈373块稠油中的溶解度、体积膨胀系数均随压力的升高而升高,且CO2的溶解性能、膨胀幅度均优于N2;随压力的增大,溶解气体后原油黏度呈下降趋势,且CO2的降黏效果优于N2;在油藏压力12 MPa下,N2的溶解可使原油的黏度下降23.58%,CO2溶解可使原油黏度下降74.96%。注CO2吞吐、N2吞吐和蒸汽吞吐的平均周期产油量由高到低依次为788.5 t、419.9 t和266.7 t,因此CO2吞吐的周期产油效果最高;从保持地层能量来看,在同一井筒范围(20 m)CO2的吞吐的油藏压力降幅最小,对补充地层能量的效果最好;从经济效益来看,N2吞吐的经济效果最高、CO2吞吐次之,蒸汽吞吐已无经济效益。     

鸡卵黄免疫球蛋白的嗜硫性色谱一步分离纯化

采用重力沉降法对卵黄液进行脱脂预处理 ,利用嗜硫性色谱 ,实现鸡卵黄免疫球蛋白(IgY)的一步分离纯化 ,蛋白质的回收率高达 91% .鸡卵黄免疫球蛋白IgY经SDS -PAGE检测为电泳纯 ,活性回收率达 70 %以上     

图与其补图谱半径之和的新上界

该文给出了图与其补图谱半径之和ρ(G)+ρ(Gc)的新上界,对任一n阶图G,有:p(G)+p(GC)≤((2-1/t)n(n-1))和p(G)+p(GC)≤((2-1/T)n(n-1))其中t=min{k,(k-)},T=max{k,(k-)},k,(k-)分别为图G和其补图Gc的色数.从而改进了[6],[8],[10]的结果.     

Recent structural studies of carbohydrate-binding modules

Carbohydrate-binding modules (CBMs) are found in many carbohydrate-active enzymes. CBMs bind to a range of polysaccharides, their primary function being to increase the catalytic efficiency of the carbohydrate-active enzymes against soluble and/or insoluble substrates. CBMs bind to their target ligands with high specificities and affinities. Thus, CBM systems are excellent models to study the mechanism of protein-carbohydrate interaction. To date, CBMs have been classified into 45 different families and many structural and functional studies have been reported. At present, three-dimensional structures of CBMs from 31 different families have been determined. These structures demonstrate that the fold most commonly found in CBMs is the β-sandwich. In the past few years, about 10 new structures from different families have been reported. These enable detailed classification of CBM structures. This article reviews recent structural and functional studies of CBMs and discusses the sub-classification of β-sandwich CBMs. Received 28 April 2006; received after revision 12 July 2006; accepted 14 September 2006     

水光互补基地水库调度图绘制及应用策略

针对水光互补基地调度问题，提出了一种新的水库调度图绘制方法，该方法根据来水频率分组进行水库调度图绘制，并给出了水库调度图的应用策略。澜沧江上游西藏段实例应用结果表明，该方法所得结果符合工程实际要求，有利于电网的稳定运行，提高了混合发电系统的发电效益。     

在8位微程序控制的模型计算机中Booth算法的实现

描述了在8位微程序控制的模型计算机中,通过编程实现了Booth算法的运算过程。对Booth算法进行了分析,绘出了实现Booth算法的流程图,编写了汇编语言程序,在8位微程序控制的模型计算机中实现了Booth算法,达到了预期的结果。     

图的全局2-彩虹控制的一个注记

为了研究树T的全局2-彩虹控制数γ gr2 (T)与2-彩虹控制数γ r2 (T)间的关系，分析了图的结构，采用分类讨论法和反证法，完全刻画了γ gr2 (T)=γ r2 (T)+1成立的直径等于5的所有树T，解决了Amjadi等人2017年未解决的问题。