广东省异常血红蛋白研究Ⅺ 三例Hb G Honolulu的一级结构与功能研究

用快原子轰击抽谱技术测定3例Hb G Honolulu(α_2~(30Gly→Gln)β_2)的一级结构。经氧平衡曲线测定,发现此异常血红蛋白氧亲和力与Hb A的相同,2,3-DPG对它的效应亦正常。     

Hemoglobin Biocatalyst Polymerization of Aniline by UV-vis Spectraphotometry

A biocatalytic route for the synthesis of a water soluble and conducting complex of polyaniline (PAN1)/sodium dodecylsulfate (SDS) is presented. Hemoglobin is used in SDS anion surfactant . The polymerization proceeded quickly to result in a homogeneous emeraldine-colored dispersion of PANI. Absorption spectra of PANI/SDS in the aqueous dispersions at various pH values confirms that the reversibility of dedoping and redoping process of PANI/SDS and shows that the transition from emeraldine salt to emeraldine base form occurred at about pH 10.0-11.0 in this anionic micellar system. PANI/SDS in the organic solutions dedoped initially and redoped after 24h.     

Functional Imaging of Breast Tissue and Clinical Application

0Introduction Near infrared(NIR)techniquesarebasedonsensitive,quantitativemeasurementsoffunctionalcontrastbe tweenhealthyanddiseasedtissue.Recently,researchersshowgreatinterestinmeasuringfunctionalpropertiesofbreasttis suesuchashemoglobinconcentrationoroxygensaturationbyNIRspectroscopyandNIRimaging[1,2].NIRlightcanpene trateseveralcentimetersintotissuebeforeitisattenuatedbe lowdetection.ThemainintrinsicmechanismsofNIRlightat tenuationintissuearethescatteringduetoindexofrefractionvariatio…     

Spectroscopic studies on interaction of hemoglobin and serum albumin with nicotine

The interactions of nicotine and Hb/SA were studied in vitro by UV/Vis, fluorescence, 1H NMR and FT-IR spectroscopies. The UV/Vis absorbance of Hb/SA (200 nm)shifted to red and decreased gradually with the addition of nicotine, indicating that the protein conformational change resulted from the chemical interaction. With increasing nicotine concentration, incubation of SA with nicotine caused the quenching of fluorescence typical of protein tryptophan residues, which meant that the vicinity of the tryptophan residues of SA was changed because of nicotine. FT-IR spectra showed that α-helix component of Hb/SA decreased, turn and β-structure components of Hb/SA increased in the presence of nicotine. In the 1H NMR spectra of nicotine, all proton peaks on pyrrolidinyl ring moved to downfield and the resonance emanating from nicotine was preferentially broadened while the concentration of Hb/SA increased. All these results indicate that nicotine and Hb/SA in vitro interact on each other, forming a new complex and inducing the protein conformational change.     

Early circulating erythroid progenitors (BFU-E) in sickle cell anemia

Sickle cell anemia (SS) patients can be divided into two sub-populations according to peripheral HbF levels. Patients with low (<9%) HbF levels (LFSS) are characterized by an increased number of circulating BFU-E in active DNA synthesis, and release of burst promoting activity (BPA) by unstimulated low density (LD) adherent cells. In contrast, circulating BFU-E from SS patients with high (>9%) HbF levels (HFSS) are normal in number, largely in resting phase, and their LD cells do not release BPA-like activity.More recently further heterogeneity has been found among these two groups. In LFSS patients GM-CSF is constitutively produced by unstimulated monocytes. In contrast, HFSS patients' adherent cell depletion increases cycling of BFU-E in culture. CM from HFSS patients inhibits BFU-E expression in culture. Hence, LD adherent cells from HFSS patients may release an inhibitory factor(s). The nature of this factor has to be determined.In addition, there are distinct subpopulations of BFU-E responsiveness to growth factor (GM-CSF, IL-3): a) LFSS patients have a homogeneous BFU-E population, equally responsive to GM-CSF and IL-3; b) HFSS patients, in addition to this subpopulation, have a subset of BFU-E dependent exclusively on IL-3 which is 20 to 40% of the total number of circulating BFU-E. This is similar to BFU-E from normal individuals. Hence, LFSS BFU-E represent an actively proliferating population, equally responsive to GM-CSF and IL-3, controlled by at least constitutively produced GM-CSF and possibly other factors.These observations suggest a significant modification in BFU-E behavior in the subset of SS patients with low HbF levels and high hemopoietic stress. The heterogenous regulation of BFU-E in SS disease seems to be an epiphenomenon of HbF levels, and not vice-versa.     

Fluorescence spectra and enzymatic property of hemoglobin as mimetic peroxidase

0　IntroductionHemoglobin(Hb)isthemajorhemeproteinofredbloodcells(RBCs)andisresponsibleforthetransportofoxygentothetissues.ThefunctionofHbdependsupontheabilityoffer rousironinthehemegrouptobindandreleaseoxygen .Despiteitsprincipalroleasanoxygen carrier,theHbmoleculepossessesdifferentenzymaticactivities[1 ] andamethodwasdevelopedforthedeterminationofHbbasedonitsenzymaticactivityfortheox idationofo phenylenediamine (OPDA)withH2 O2 asanoxi dant[2 ] .Hbasamimeticenzymeofperoxidase ,cancataly…     

广东省异常血红蛋白研究:Ⅰ、对广东部分地区83510人异常血红蛋白调查报告

我们从1981年开展广东异常血红蛋白调查,到1985年底共调查人数83510人,他们是梅县、韶关地区以及暨大附近几个大学的学生,从中发现425例异常Hb。发现率比全国平均数高。在这425例中有HbG 57例,HbK 135、HbG(D)118、HbE 106、HbQ 9例。除极少数例外,绝大多数患者都没有任何临床症状。此外祗要可能,都作了家系调查,发现有双重遗传,文中列出五个家系。     

双羔滩羊血红蛋白多态性的研究

采用聚丙烯酰胺凝胶电泳技术分析滩羊双羔系和单羔系血红蛋白（Ｈｂ）多态性的差异．结果表明，Ｈｂ位点鉴别出的两个等位基因ＨｂＡ和ＨｂＢ控制着三种表现型（基因型），其中实验组２８只产双羔家系滩羊的基因型频率比对照组的６２只产单羔家系滩羊的基因型频率ＨｂＡＡ低１．２７％，ＨｂＡＢ低９．５６％，ＨｂＢＢ高１０．８３％，而基因频率实验组比对照组的ＨｂＡ低６％，ＨｂＢ高６％，因而两组的基因型频率和基因频率均有差异．     

老年女性血红蛋白正常参考值与中国地理因素

为了给制定中国老年女性血红蛋正常参考值的统一标准提供科学依据.收集了中国157个单位及氰化高铁蛋白(HiCN)法测定的15690例老年女性血红蛋白正常参考值,运用相关分析和回归的方法,研究了其与地理因素的关系.结果发现老年女性血红蛋白正常参考值与中国地理因素之间有很显著的相关关系(F=168.99).用逐步回归分析的方法推导出了一个回归方程:(＾Y)=13.50 0.00606X1-1.132X4 0.006463X5±16.5用该回归方程可以估算中国某个地区的老年女性血红蛋白正常参考值.依据血红蛋白正常参考值与地理因素的依赖关系把中国分为青藏区、西南区、西北区、东南区、华北区、东北区等六个区.     

基于硅胶载体表面分子印迹聚合物对牛血清蛋白识别性能

以硅胶作为载体,丙烯酰胺和2-丙烯酰胺基-2-甲基丙磺酸为双功能单体,牛血清蛋白质作为模板分子,制备了牛血清蛋白表面分子印迹聚合物,并对其制备方法、性状和性质进行了检测。结果证明制备的分子印迹聚合物大小在400 nm左右,当功能单体的配比为5∶1时为最优配比,分子印迹聚合物对牛血清蛋白质的结合在60 min时达到平衡,并且在浓度为0. 6mg·m L~(-1)的情况下,结合率达到饱和。特异性检测可知分子印迹聚合物对牛血清蛋白的结合率最高并且其印迹因子(IF)高达1. 58,说明了它对目标蛋白具有良好的选择识别能力,且重复性能好,重复检测后吸附量在80%左右。