沙枣叶水煎提取物体外抗氧化作用的研究

本实验通过自发产生脂质过氧化及分别用自由基诱导剂CCl4、H2O2和Fe2+-VitC激发脂质过氧化,以丙二醛(MDA)产生量作为脂质过氧化作用程度指标,观察沙枣叶水煎提取物对小鼠肝脂质过氧化的保护作用强弱.结果显示沙枣叶水煎提取物可降低小鼠肝组织自发性MDA的生成,减轻CCl4 、H2O2 、Fe2+-VitC所诱导的肝脏脂质过氧化反应,具有良好的抗脂质过氧化作用.     

富硒乳酸菌抗肝损伤小鼠红细胞脂质过氧化作用

选择60只健康雌雄对半成年小鼠，随机分成对照组(C组)，富硒乳酸菌组(Se组)，CCl4-富硒乳酸菌组(CCl4-Se)，CCl4注射组(CCl4组)，通过腹腔注射CCl4诱发肝损伤，分别在第2、4、6周采集血样并测定红细胞溶血液中GSH-Px、GST和SOD活性及MDA含量．研究富硒乳酸菌对CCl4诱发肝损伤小鼠红细胞脂质过氧化损伤的保护作用．结果显示，在整个实验期内，SOD各组间未见明显差异；GST表现为Se组最高，呈上升趋势，且在第6周高于或明显高于其他符组，CCl4组低于或明显低于C组和CCl4-Se组，C组和CCl4-Se组相近；C组、Se组和CCl4-Se组GSH—Px活性无明显差异，而CCl4组随负荷时间呈下降趋势，第4周后明显低于其余三组．C组和CCl4-Se组的MDA无明显差异，Se组整体水平低于或明显低于其它三组，CCl4组高于或显著高于其它三组．提示CCl4诱发肝损伤小鼠红细胞脂质过氧化变化剧烈，富硒乳酸菌制剂能通过抗损伤保护以及增强抗氧化酶活性发挥有效作用．     

三种海藻体外抗氧化活性的研究

在体外化学模拟环境的条件下,采用比色法测定了羊栖菜,条浒苔和坛紫菜醇提物对DPPH自由基,羟基自由基和超氧阴离子的清除能力以及抗脂质过氧化能力。羊栖菜醇提物显示出较强的DPPH自由基清除能力,羟基自由基清除能力与抗脂质过氧化能力,坛紫菜醇提物显示出较强的超氧阴离子自由基清除能力与抗脂质过氧化能力。     

柿叶提取物对CCl4致小鼠急性肝损伤保护作用的研究

探讨了柿叶醇提物的乙酸乙酯萃取制剂(EAF)对小鼠急性肝损伤的保护作用.腹腔注射四氯化碳(CCl4)致小鼠急性肝损伤模型,测定不同剂量的EAF对血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)活性,肝超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量的影响,同时对肝组织进行病理学检查.结果表明,EAF具有剂量依赖性地降低血清中ALT、AST、AKP值,增强肝中SOD、GPx、CAT的活性,降低肝组织匀浆中MDA的含量,减轻CCl4对肝脏细胞的病理损伤.100mg/kg是EAF对肝损伤的合适保护剂量.适量的EAF对CCl4所致小鼠急性肝损伤有较好的保护作用.     

2—乙基—3—羟基—6—苯硫基—4（1H）—吡啶酮对动物实验急性肝损伤的保护作用

目的：研究2－乙基－3－羟基－6－苯硫基－4（1H）－吡啶酮（HPP）对四氯化碳(CCl4),硫代乙酰胺（TAA）及D－氨基半乳糖（D－GalN)引发的小鼠急性肝损伤的影响。方法：用CCl4,TAA,D-GalN引发动物急性肝损伤（D－GalN)引发的小鼠急性肝损伤的影响。方法：用CCl4,TAA,D-GalN引发动物急性肝损伤,HPP通过口服红药,测定动物血清中谷丙转氨酶（GPT）,谷草转氨酶（GOT）,谷胱甘肽过氧化物酶（GSH－Px) ,超氧化物歧化酶（SOD）的活性,用硫代巴比妥酸法测定肝组织中丙二醛（MDA）含量。结果：HPP1.6mg.kg^-1,6.4mg.kg^-1显著抑制运物血清中GPT,GOT活性的升高,显著抑制GSH－Px,SOD活性的降低且能降低肝组织中脂质过氧化产物MDA含量的升高,同时HPP对CCl4所致大鼠急性肝损伤也有一定的抑制作用,实验表明HPP对正常小鼠血清及肝脏GPT活力无明显影响,结论：HPP对动物急性肝损伤有明显的抑制作用。     

壳寡糖及其衍生物对CCl4诱导的小鼠肝损伤的保护作用

研究壳寡糖（COS）、氨基葡萄糖（GlcNH2）和N-乙酰氨基葡萄糖(GlcNAc)对CCl4诱导的雄性昆明种小鼠肝毒性的保护作用，并探讨其可能机制. 小鼠腹腔注射CCl4（20mg/kg体重）24h后，血清天门冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性明显提高，引起肝脏脂质过氧化反应，巯基含量降低，总抗氧化能力（T-AOC）减弱，诱发基因毒性. 提前连续12天分别灌胃给予COS，GlcNH2 和GlcNAc（1.5g/kg体重）能够显著诱导金属硫蛋白（MT）的表达，体内的抗氧化防御系统随之增强以抵抗CCl4诱导的氧化损伤. 血清AST和ALT活性明显降低，肝脏丙二醛（MDA）生成被抑制，巯基含量，T-AOC明显恢复. 但是，从DNA电泳结果反应出的基因毒性并未减轻. 实验结果证明，提前给予COS，GlcNH2和GlcNAc对CCl4诱导的小鼠肝损伤能够起到有效的保护作用，其中，GlcNH2的作用效果最显著.     

黄芪注射液对四氯化碳损伤小鼠肝的保护作用

观察黄芪注射液(HQI)对四氯化碳(CCl4)所致肝损伤的保护作用.采用CCl4所致肝损伤模型,并测定功能,肝组织学检查及肝组织匀浆脂质过氧化.结果为,CCl4模型组小鼠血清GPT为(945.7±146.1)U/100mL,显著高于正常对照组(P＜0.01).HQI低、高剂量组的血清GPT分别为(576.3±97.4)U/100mL和(518.6±78.8)U/100mL,均显著低于模型组(P＜0.01).HQI治疗组肝组织MDA浓度显著低于模型组,病理学损伤减轻.结论为,注射3g/kg～10g/kgHQI可减轻CCl4引起的小鼠肝脏损伤.本实验结果提示,HQI具有减少脂质过氧化物产生的作用.     

谷胱甘肽过氧化物酶模拟物6-CySeCD对过氧化氢诱导小鼠成纤维细胞损伤的保护作用

考察不同剂量的H2O2作用于小鼠成纤维细胞(NIH3T3)后,细胞脂质过氧化、存活率、DNA片段化的变化情况,并研究谷胱甘肽过氧化物酶(GPX)的模拟物6A,6B-环己胺-6A′,6B′-硒桥联-β-CD(6-CySeCD)抗H2O2诱导小鼠成纤维细胞损伤的能力.结果表明:H2O2对NIH3T3细胞有严重损伤;6-CySeCD能有效保护细胞,防止紫外线引起的损伤.     

裸燕麦皂苷的提取分离及其抗氧化活性

从裸燕麦麸皮中提取得到总燕麦皂苷,研究了乙酸乙酯对异黄酮的脱除效果,采用薄层色谱法对裸燕麦总皂苷进行了初步分离,并研究了裸燕麦皂苷对卵磷脂脂质过氧化、小鼠肝自发性脂质过氧化和Fe2+-H2O2诱导的小鼠肝脂质过氧化的抑制作用.结果表明,乙酸乙酯可有效去除异黄酮,裸燕麦总皂苷中有6种皂苷类化合物,裸燕麦皂苷对卵磷脂脂质过氧化、小鼠肝自发性脂质过氧化和Fe2+-H2O2诱导的小鼠肝脂质过氧化均具有极显著的(P<0.01)抑制作用,抑制率分别达到58.7%、42.4%和18.6%.     

2-乙基-3-羟基-6-苯硫基-4(1H)-吡啶酮对动物实验性急性肝损伤的保护作用

目的 :研究 2 -乙基 - 3-羟基 - 6-苯硫基 - 4 ( 1H) -吡啶酮 (HPP)对四氯化碳 (CCl4 )、硫代乙酰胺 (TAA)及D -氨基半乳糖 (D -GalN)引发的小鼠急性肝损伤的影响 .方法 :用CCl4 、TAA、D -GalN引发动物急性肝损伤 ,HPP通过口服给药 ,测定动物血清中谷丙转氨酶 (GPT)、谷草转氨酶 (GOT)、谷胱甘肽过氧化物酶 (GSH -Px)、超氧化物歧化酶 (SOD)的活性 ,用硫代巴比妥酸法测定肝组织中丙二醛 (MDA)含量 .结果 :HPP 1.6mg·kg- 1、6.4mg·kg- 1显著抑制动物血清中GPT、GOT活性的升高 ,显著抑制GSH -Px ,SOD活性的降低且能降低肝组织中脂质过氧化产物MDA含量的升高 ,同时HPP对CCl4 所致大鼠急性肝损伤也有一定的抑制作用 ,实验表明HPP对正常小鼠血清及肝脏GPT活力无明显影响 .结论 :HPP对动物急性肝损伤有明显的抑制作用     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

The Analects and the Moment in Peking——The influence of a Confucian education system on the personality formation,the way of thinking and the fate of the main characters

0.Preface China has long been boasting of its colorful and immense culture which comprises of Taoism,Confucianism and Buddhism.The interwoven influence among the religions on Chinese way of thinking,on the nation＇s behavior in their social life throughout the long history is beyond list.