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1.
番红花花被和花柱,在含4mg/LKT,3mg/LNAA和0.6mg/L4PU-30的MS培养基中可定向再生红色花柱-柱头状物,花被的诱导频率大于花柱;长度为22~66mm的花芽具有较高的诱导频率(34.4%).番红花球茎在含1.4mg/LNAA和0.6mg/LKT或0.8mg/L2,4-D的B5和MS培养基上,均能诱导出颗粒状愈伤组织,愈伤组织在附加0.6~1.4mg/LNAA的MS培养基上,既可形成小球茎,也能长出完整植株  相似文献   

2.
杂种石斛兰组织培养的研究   总被引:10,自引:0,他引:10  
本文用引进的泰国石斛兰熊猫1号(PANDANo.1)与熊猫2号(PANDANo.2)杂交,并将杂交种子经组织培养,获得无病毒试管苗结果表明:在附加6-BA0.1~1mg/L和NAA0.5~1.5mg/L的改良KC及改良MS培养基上,原球茎的发生率达90%以上,增殖8~10倍.生根壮苗培养阶段,以附加5%~10%马铃薯提取液和5%~10%香蕉汁(不加任何激素)的处理效果最佳.用冷开水代替蒸馏水,食用白糖代替试剂蔗糖,对杂交石斛兰试管苗的生长无明显影响.  相似文献   

3.
用离体培养无性繁殖苦瓜   总被引:21,自引:0,他引:21  
切取由苦瓜种子形成的试管苗的顶芽及带芽茎段,移植于附加4 ̄6mg/L ZT或4 ̄6mg/L 6-BA的MS培养基上,可以促进芽的增殖,形成芽簇,每月每株可增殖4苗;移植于附加4mg/L ZT及0.5mg/L KT或附加4mg/L ZT及LH(水解乳蛋白)的培养基上,每月每株可增殖5苗。将幼苗接转于MS+ZT0.5mg/L的培养基上,生长健壮。丛芽生根选用大量元素减半的MS培养基,蔗糖浓度为2%效果  相似文献   

4.
荞麦的组织培养及植株再生   总被引:3,自引:0,他引:3  
荞麦(Fagopyrum esculentum Moench.)无菌苗下胚轴切段在含不同激素配比的MS培养基上进行愈伤组织诱导,发现在 2. 0 mg/L 2. 4-D与 1. 5 mg/L 6 BA组合时,可100%地诱导出愈伤组织。愈伤组织在含 1.5mg/L 6BA和1.5 mg/L 2.4-D的激素条件下继代培养1代后转入含2mg/L 6 BA和1mg/L KT的 MS培养基上,计有80%以上可分化出苗。转入0.2mg/L NAA的 1/2MS培养基上则可诱导出根。研究建立了荞麦离体诱导高频率再生植株的实验体系。  相似文献   

5.
生长调节物质对丹参叶片脱分化及根芽分化的效应   总被引:11,自引:1,他引:11  
生长调节物质对丹参叶片脱分化及根芽分化效应的研究结果表明:(1)0.5~5.0mg/L2,4-D,0.1~0.5mg/LNAA0.5~1.5mg/LIBA或0.2~1.0mg/L4PU-30诱导叶片的脱分化率高达97%~1000%。(2)0.1~0.5mg/LNAA或0.5~2.0mg/IBA诱导芽化率为93%~100%,3种细胞分裂素中,4PU-30对叶片芽化最有效,6-BA和KT次之。(3)随  相似文献   

6.
不同植物生长调节物质对华黄芪愈伤组织形成及器官发…   总被引:3,自引:0,他引:3  
以华黄芪为材料,MS为基本培养基,设计不同浓度的NAA、2,4-D、6-BA、KT等单因子试验和不同比值的细胞分裂素/生长素组合试验,探讨植物生长调节物质对华黄芪愈伤组织形成及器官发生的效应。结果表明:在愈伤组织形成过程中,单因子试验以5.0mg/L NAA、0.5mg/L2,4-D、1.0mg/L 6-BA、20mg/L KT诱导效果较好,诱导频率可达100%,且生长势好。组合试验比单因子试验能  相似文献   

7.
温桂珍 《广西科学》1995,2(2):62-63
以巴西铁(Dracaena sanderiena)茎段为接种材料,MS为基本培养基,吲哚乙酸(IAA)、茶乙酸(NAA),2,4-二氯苯氧乙酸(2.4-D)、6-苄氨基嘌呤(6-BA)、6一糠基氨基嘌呤(6-FA)、吲哚丁酸(IBA)、生根粉(ABT1)、玉米素(ZT)为附加成分,进行诱导、分化、生根试验。诱导结果表明:IAA不起作用,2,4-D有较好的促进作用,0.5mg/L(2,4-D)+2mg/L(6-BA)组合,诱导率高达90%。分化结果表明:NAA、6-BA效果较好,6-FA作用不明显,lmg/LNAA+4ms/L(6-BA)+2ms/LZT组合,分化率最高.达81.1%。生根结果表明:MS+0.5mg/LNAA+0.5mg/L(6-FA),MS+0.4mg/LNAA+2mg/LIBA,MS+0.2mg/LNAA+0.1mg/L(6-FA)+ABT1三种培养基均发根,生根率70%以上,根粗,但有点脆,若培养基中加入活性碳产生的根则细而韧。  相似文献   

8.
MS培养基附加BA1,0~2.2/NAA0.01~0.1mg/L、KT1.0~2.0/NAA0.01~0.1mg/L、BA0.5+KT0.5/NAA0.1mg/L及BA1.0+KT1.0/NAA0.1mg/L的不同激素组合,处理山丹离体子叶,均能诱导其产生愈伤组织和分化芽,除3A1.0/NAA0.01和BA1.0/NAA0.1外,各组合还均能诱发生根和使植株再生。  相似文献   

9.
荞麦组织培养中的植株再生   总被引:1,自引:0,他引:1  
用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株。实验结果表明,适宜诱导愈伤组织的激素组合为2.4-D4.0mg/L+KT0.2mg/L,适宜愈伤组织生长的激素组合为2.4-D1.0mg/L+KT1.0mg/L。诱导芽的较适宜的组合为6-BA3.0mg/L+IAA0.1mg/L,不定芽接种到含MS+IBA2.0mg/L+KT0.1mg/L的MS培养基上就能生  相似文献   

10.
芝麻下胚轴愈伤组织的形成和器官发生   总被引:4,自引:0,他引:4  
本文研究了五种植物激素对芝麻下胚轴愈伤组织的形成和器官发生的影响.研究表明:(1)2.4-D或NAA(1mg/L)+BA或KT(0.smg/L),2.4-D(0.5-1mg/L)和NAA(05-1mg/L)对愈伤组织的诱导率最高;(2)BA或KT(0.5-1mg/L)可促进下胚轴芽丛的形成(3)NAA(0.5-1mg/L)、IAA(0.5-1mg/L)和KT(0.5-1mg/L)对下胚轴根的形成最有利.  相似文献   

11.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

12.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

13.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

14.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

15.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

16.
17.
The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.  相似文献   

18.
R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.  相似文献   

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