| 荞麦的组织培养及植株再生 |
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| 引用本文： | 金红,郝建国.荞麦的组织培养及植株再生[J].西北大学学报,1999,29(6):611-614. |
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| 作者姓名： | 金红 郝建国 |
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| 作者单位： | 西北大学生物技术研究所!陕西西安710069 |
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| 基金项目： | 陕西省教委重点攻关项目!97JZK15，国家自然科学基金!39770365 |
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| 摘 要： | 荞麦（Ｆａｇｏｐｙｒｕｍ ｅｓｃｕｌｅｎｔｕｍ Ｍｏｅｎｃｈ．）无菌苗下胚轴切段在含不同激素配比的ＭＳ培养基上进行愈伤组织诱导，发现在 ２． ０ ｍｇ／Ｌ ２． ４－Ｄ与 １． ５ ｍｇ／Ｌ ６ ＢＡ组合时，可１００％地诱导出愈伤组织。愈伤组织在含 １．５ｍｇ／Ｌ ６ＢＡ和１．５ ｍｇ／Ｌ ２．４－Ｄ的激素条件下继代培养１代后转入含２ｍｇ／Ｌ ６ ＢＡ和１ｍｇ／Ｌ ＫＴ的 ＭＳ培养基上，计有８０％以上可分化出苗。转入０．２ｍｇ／Ｌ ＮＡＡ的 １／２ＭＳ培养基上则可诱导出根。研究建立了荞麦离体诱导高频率再生植株的实验体系。
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| 关 键 词： | 荞麦 组织培养 愈伤组织 再生植株 |
The tissue culture and regeneration of buckwheat (Fagopyrum esculemtum Moench) |
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| JIN Hong, HAO Jian-guo, MA Hong-jun, JIA Jing-fen.The tissue culture and regeneration of buckwheat (Fagopyrum esculemtum Moench)[J].Journal of Northwest University(Natural Science Edition),1999,29(6):611-614. |
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| Authors: | JIN Hong HAO Jian-guo MA Hong-jun JIA Jing-fen |
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| Abstract: | The hypocotyls of buckwheat seedlings were sliced and placed on MS medium containing different phytohormones to induce callus. It was found that callus could be induced efficiently on MS medium supplemented with 2. 4-D 2. 0 mg/L and 6BA 1. 5 mg/L. High frequency of shoot differentiation was obtained on MS medium plus 6BA 2mg/L and KT 1mg/L. When the shoots were transferred on 1/2 MS medium added with NAA 0. 2 mg/L, roots were induced. This work provides a high efficient regeneration system in buckwheat. |
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| Keywords: | buckwheat tissue culture callus plant regeneration |
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