筛选假单胞菌株M18防治大棚黄瓜枯萎病害

设计了一种新的高效筛选程序,从植物根际土壤中分离出一株具有ＡＣＣ脱氨酶活性的促生拮抗双功能假单胞菌株Ｍ１８．在培养皿中,Ｍ１８能有效地抑制黄瓜枯萎病菌以及多种其他枯萎病原菌和植物病原菌的生长．Ｍ１８活菌浸种处理春黄瓜种子以及在大棚定植后根际土壤浇灌的试验中,与对照组相比,黄瓜枯萎病的株发病率降低７０％～８０％,霜霉病的发病率和病情指数都同时平均下降７０％左右,黄瓜产量提高２０％以上,每公顷可增加产值０．９万元以上,Ｍ１８的生物防治效果达到极显著程度．     

具有ACC脱氨酶活性及抗枯萎病菌的假单胞菌株B*8

运用新的快速筛选程序,获得了具有１－氨基环丙烷－１－羧酸（１－ａｍｉｎｏｃｙｃｌｏｐｒｏｐａｎｅ－１ｃａｒ－ｂｏｘｙｌａｔｅ,ＡＣＣ）脱氨酶活性和拮抗瓜类枯萎病菌双重功能的根际假单胞菌株Ｂ８．利用ＡＣＣ为唯一氮源,从不同的土样中,快速筛选出８０个具有ＡＣＣ脱氨酶活性的菌落．在马铃薯葡萄糖培养基和西瓜培养基上,经异步培养法进一步筛选并纯化出３株对西瓜枯萎病菌具有较强拮抗作用的拮抗菌Ａ９、Ｂ８和Ｃ１７．对拮抗作用最强的Ｂ８进行分析表明,它属于假单孢菌属,能分泌较多的铁载体,对瓜类枯萎病以及其他植物病原菌具有广谱抗性．结果显示,此程序可替代通常采用的低效而耗时的筛选和生物测定过程,加快新的促进植物生长根际细菌的筛选和应用．     

荧光假单胞菌的应用与展望

植物根际促生菌(PGPR)中的荧光假单胞菌(Pseudomonas fluorescens)对植物病原体具有潜在的拮抗作用,同时具有促进植物生长的能力.阐述了荧光假单胞菌在实际生产中的应用潜力.荧光假单胞菌可以通过产生生长素,增强土壤中磷、钾的可用性,促进植物叶绿素含量的增加,及产生谷胱甘肽、ACC脱氨酶等形式促进植物的生长.通过产抗生素(吩嗪、2,4-二乙酰基间苯三酚、藤黄绿脓菌素、硝吡咯菌素)、拮抗线虫等方式帮助植物体抵抗病虫害的侵袭.除此之外,荧光假单胞菌所具有的定殖与产铁载体能力在植物的促生与抗病方面皆发挥着重要作用.荧光假单胞菌在农业生产方面具有巨大的应用潜力.     

几株光合细菌DAN的G＋Cmol%及DNA—DNA同源性的测定

对８株新分离的光合细菌从形态，生理及遗传学特性等方面进行了研究，结果表明，８株菌可以分为５个不同的ＤＮＡ同源群，其中菌株１３，１８，３７，４５可以划分为一个同源群，它们与沼泽红假单胞菌（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓｐａｌｕｓｔｒｉｓ）模式菌株ＡＴＣＣ１７００１的ＤＮＡ同源性在７０％以上，菌株８，４０，５２，５５分别形成另外四个同源群，菌株８与深红红螺菌（Ｒｈｏｄｏｓｐｉｒｉｌｌｕｍｒｕｂｒｕｍ     

迷迭香抗植物病原菌活性研究

用滤纸片扩散法和菌丝生长速率法实验研究了迷迭香乙醇提取物对6种植物病原真菌菌丝生长的抑制作用。结果表明迷迭香乙醇提取物对水稻稻瘟病菌、番茄霜霉病菌、黄瓜枯萎病菌有较好的抑制活性,浓度在5mg／mL以上时,抑菌率达到100％。     

红假单胞菌的分离鉴定与生长特性

由青岛沿岸潮间带表层沉积物分离出４株光合细菌，经鉴定属于荚膜红假单胞菌和球形红假单胞菌。光照厌氧培养生长实验表明ＨＤ３的最适盐度为Ｓ＝１５－２５，ＰＳ２－２为一淡水菌株。菌株ＨＤ３的最适ＰＨ范围为６．３５－８．３。ＨＤ３、ＲＳ均不能利用硫化物且其生长受较高浓度的硫化物抑制。     

上海地区不同植物根际荧光假单胞菌多样性

荧光假单胞菌是一种常见的植物根际促生细菌,有开发作为生物农药的潜质．以分离自上海周边地区12种植物根际的104株荧光假单胞菌为研究对象,进行了表型分类,并采用16S—rDNARFLP和REP—PCR技术,系统地研究了荧光假单胞菌的遗传多样性．结果表明：16S—rDNARFLP酶切图谱聚类分析在85％相似性水平上,可将供试菌株划分为9群;REP—PCR指纹图谱聚类分析在76％相似性水平上,可将供试菌株划分为26群,说明上海地区的荧光假单胞菌具有丰富的遗传多样性;聚类分析结果还显示出菌株间亲缘关系与其分离地和所在植物具有相关性,表明环境条件对荧光假单胞菌的发育和遗传分化有重要影响．     

探究促生根际菌(PGPR)对黄瓜生长的影响

促生根际菌-Plant Growth Promoting Rhizbacteria菌(简称PGPR),是一种能够促进植物生长、防治病害、增加作物产量的微生物.本试验引进PGPR菌种资源技术,以黄瓜的生长为研究对象,探究了促生根际菌(PGPR)对黄瓜生长的影响.结果表明,在试验中采用的两种促生根际菌假单胞细菌(Pseudomonas putida)和肠杆菌(Enterobacter cloacae)对黄瓜生长的影响不明显.     

探究促生根际菌(PGPR)对黄瓜生长的影响

促生根际菌——Plant Growth Promoting Rhizbacteria菌(简称PGPR),是一种能够促进植物生长、防治病害、增加作物产量的微生物。本试验引进PGPR菌种资源技术,以黄瓜的生长为研究对象,探究了促生根际菌(PGPR)对黄瓜生长的影响。结果表明,在试验中采用的两种促生根际菌假单胞细菌(Pseudomonas putida)和肠杆菌(Enterobacter cloacae)对黄瓜生长的影响不明显。     

红树林土壤中假单胞菌群的重金属耐受活性

本研究从广西钦州茅尾海红树林保护区土壤中分离出的假单胞菌群着手，对其进行重金属耐受性能筛选，旨在获得可对重金属污染环境进行高效修复的菌株。选用传统稀释涂布法和16S rRNA基因序列的系统发育分析红树林土壤中的可培养假单胞菌；通过肉汤稀释法对菌株展开6种重金属耐受特性研究，对其进行耐受基因检测，并进一步测试重金属耐受活性显著菌株的重金属吸附性能。结果表明，从红树林土壤中共获得23株假单胞菌，18株菌对至少一种重金属表现出耐受活性，总阳性率为78.26%；10株菌的基因组DNA中检测出重金属耐受基因。其中，对铅和/或锌耐受活性显著的11株菌中，Y234、Y123、Y264、Y225和Y346具有显著的吸附性能，铅和/或锌的吸附率均大于50%。广西钦州茅尾海红树林保护区土壤中可培养假单胞菌群丰富，其重金属耐受活性显著，具有较大的挖掘潜力。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.