红树林土壤中假单胞菌群的重金属耐受活性

本研究从广西钦州茅尾海红树林保护区土壤中分离出的假单胞菌群着手，对其进行重金属耐受性能筛选，旨在获得可对重金属污染环境进行高效修复的菌株。选用传统稀释涂布法和16S rRNA基因序列的系统发育分析红树林土壤中的可培养假单胞菌；通过肉汤稀释法对菌株展开6种重金属耐受特性研究，对其进行耐受基因检测，并进一步测试重金属耐受活性显著菌株的重金属吸附性能。结果表明，从红树林土壤中共获得23株假单胞菌，18株菌对至少一种重金属表现出耐受活性，总阳性率为78.26%；10株菌的基因组DNA中检测出重金属耐受基因。其中，对铅和/或锌耐受活性显著的11株菌中，Y234、Y123、Y264、Y225和Y346具有显著的吸附性能，铅和/或锌的吸附率均大于50%。广西钦州茅尾海红树林保护区土壤中可培养假单胞菌群丰富，其重金属耐受活性显著，具有较大的挖掘潜力。

Study on Heavy Metal Tolerance Activity of Pseudomonas Species Isolated from Mangrove Soil

The purpose of this study was to investigate Pseudomonas species collected from the Maoweihai natural reserve of mangrove in Qinzhou,Guangxi,and screen the heavy metal tolerance activity from these strains.It was intended to obtain the strains that can effectively repair the environment polluted by heavy metals.The culturable Pseudomonas species in mangrove soil were analyzed by traditional dilution coating method and phylogenetic analysis based on 16S rRNA gene sequences.The tolerance characteristics of six heavy metals were screened by broth dilution method.Tolerance genes were detected,and the heavy metal adsorption properties of heavy metal tolerant strains were further tested.The results showed that a total of 23 Pseudomonas strains were obtained from mangrove soil.Among them,18 strains showed the tolerance activity to at least one heavy metal,with the total positive rate of 78.26%.The heavy metal tolerance genes were detected in genomic DNAs of 10 strains.Among the 11 strains with significant tolerance to lead and/or zinc,Y234,Y123,Y264,Y225 and Y346 had significant adsorption properties,and the adsorption rates of lead and/or zinc were more than 50%.There are abundant culturable Pseudomonas species in the soil of Maoweihai Mangrove Reserve in Qinzhou,Guangxi.The heavy metal tolerance activities of these strains are significant,which has great potential for exploitation.