SARS冠状病毒核蛋白的质谱鉴定

利用质谱技术,对纯化的SARS冠状病毒的核蛋白进行初步鉴定与分析.所获得的质谱分析数据中,分子量为2881.19Da的酶切肽段的氨基酸序列与其它冠状病毒的同源性比较分析表明,SARS冠状病毒和其它冠状病毒均含有一个高度保守的基序“FYYLGTGP”.     

SARS及其它冠状病毒基因组组织结构和序列的初步比较分析

严重急性呼吸综合症(Severeacuterespiratorysydrome,SARS)是一种全新的传染性疾病,它可能主要是由冠状病毒的一个变种引起的.本文比较SARS及其它冠状病毒基因组组织结构和序列变异的情况,初步的结果表明:①尽管SARS病毒与冠状病毒属的另外6种病毒基因组序列上有较大的差异,但是它们具有较相似的基因组组织结构;②SARS病毒与牛冠状病毒、鼠肝炎病毒具有较近的系统发育关系;③虽然SARS病毒扩散的时间极短,但来自不同地区SARS病毒的DNA序列却存在一些突变,且这些突变又大多是改变氨基酸序列的非同义突变.     

基于密码子使用模式的冠状病毒亲缘关系分析

重症急性呼吸综合症(severe acute respiratorysyndrome,SARS),临床表现为非典型肺炎.由于SARS具有很高的传染性和一种新型的冠状病毒(SARS-CoV)被认为是引起SARS的病原体.SARS冠状病毒(SARS-CoV)属于巢状病毒目(OrderN idovirales),冠状病毒科(Fam ily Coronaviri-dae),冠状病     

SARS冠状病毒核衣壳蛋白cDNA的克隆和原核表达载体的构建

目的克隆SARS冠状病毒核衣壳(N)蛋白.方法用RT-PCR技术克隆SARS冠状病毒N蛋白全长cDNA,cDNA经序列分析鉴定后,克隆到pET30a表达载体.结果实现了SARS冠状病毒N蛋白基因的克隆.结论可以对重组成功的pET30a-N进行进一步的应用.     

冠状病毒主蛋白酶及其化学抑制剂研究进展

一个新的冠状病毒已经被鉴定为急性呼吸道综合症(SARS)的病原体,控制该病毒复制复合体活性的主蛋白酶(Mpro或3CLpro)将很可能成为开发针对SARS特效治疗药物的靶位点.综述了人冠状病毒株229E(HCoV 229E)和一种在猪体内引发传染性胃肠炎的病毒(TGEV)的Mpro的晶体结构以及以此为基础构建的SARS冠状病毒(SARS-CoV)同源蛋白酶的空间结构模型.通过对这些同源蛋白酶的结构及其与已知的蛋白酶化学抑制剂的结合特征进行分析后发现Mpro的底物结合位点具有惊人的保守性,这种保守性也被重组SARS-CoV Mpro能介导的TGEV Mpro的底物剪切实验所进一步证实.分子模型表明已有的鼻病毒3Cpro抑制剂经过改进后或许能用于SARS的治疗.     

新冠知识二十条

正面对疫情,我们需要不信谣、不传谣,在充分认识它的前提下做好扎实的防护。1.新型冠状病毒和SARS同类不同种新型冠状病毒属于β属的冠状病毒,有包膜,颗粒呈圆形或椭圆形,常为多形性,直径60-140nm。其基因特征与SARSr-CoV和MERSr-CoV有明显区别。目前研究显示与蝙蝠SARS样冠状病毒(bat-SL-CoVZC45)同源性达85%以上。新型冠状病毒是冠状病毒的一种,它跟SARS冠状病毒是平行的,二者是同一类(病毒),但不是同一种。     

支持向量机程序SVMProt预测SArs病毒蛋白质的功能

对SARS冠状病毒蛋白质功能的有效识剐将有利于促进SARS传染病治疗药物的开发。应用基于支持向量机原理的SVMProt程序识别SARS冠状病毒蛋白质的功能,通过对SARS冠状病毒中2个已知功能的蛋白质功能的成功预测,说明SVMProt能够有效地应用于SARS冠状病毒蛋白质及其他种类蛋白质的功能预测。对SARS冠状病毒中至今仍未知其功能的蛋白质ORFl3的功能进行了预测,结果显示ORFl3是一种可能与DNA结合的核蛋白并兼有病毒体内结构蛋白的功能。     

SARS冠状病毒基因芯片的制作与初步临床样本验证

为了实现对非典(SARS)冠状病毒感染患者的早期诊断,构建了一套以基因芯片为基础的SARS冠状病毒基因分析检测系统。首先从患者痰、血样本中快速分离出SARS冠状病毒的RNA,然后通过巢式RT-PCR对分离的RNA进行扩增获得特定的DNA片段,最后让固定在基因芯片上的DNA探针与扩增产物进行杂交以检验扩增产物的序列匹配性,达到最终确认SARS冠状病毒感染患者的目的。对首期两例患者痰样本和两例全血样本的分析结果表明,这4例患者样本中均存在SARS冠状病毒。     

SARS冠状病毒及相关病毒的分子系统学分析

根据SARS冠状病毒及其相关病毒的基因组核酸序列和3种不同蛋白质序列，应用最大简约法和最小进化法重建系统发育树；并对SARS冠状病毒的11个推测蛋白质(ORF)做BLAST分析。结果表明，SARS冠状病毒和鼠肝炎病毒——牛冠状病毒分支构成姊妹群。其单系群性质得到强有力的统计学支持。这暗示了SARS的爆发可能源自种间屏障的突破事件，该病毒天然宿主可能为猪、牛或鼠。SARS冠状病毒与已知的人冠状病毒分属冠状病毒科的不同分支，因此致病机制可能有很大不同。3个基因的系统树分支格局的一致性表明：SARS冠状病毒这3个主要基因与其他冠状病毒间不存在重组，但全部11个ORF的BLAST分析却认为其基因组上一些小的区段可能与其他病毒存在重组。     

中国科学家绘出SARS病毒精细"家谱"

中国科学院理论物理研究所郝柏林院士及其领导的科研小组最近采用全新的方法 ,在 SARS病毒不同毒株之间的亲缘关系以及与其它冠状病毒的进化关系研究方面取得进展。　　研究人员首先选取 6个完整的 SARS基因组和 14个已知的冠状病毒的完全基因组 (与 SARS同属Nidovirales科 ) ,利用“组分矢量”方法探讨这些基因之间的进化关系。从已经得到的进化树可以看出 ,在全基因组的层次上 ,选定的 6个 SARS毒株相互之间关系较近 ,在进化树上形成单群 ,而与已知的冠状病毒距离比较远 ,说明 SASR冠状病毒是冠状病毒属的独立种群。　　此外 ,由…     

Bioinformatical study on the proteomics and evolution of SARS-CoV

A novel coronavirus has been identified as the causative agent of the severe acute respiratory syndrome (SARS). For all the SARS-CoV associated proteins derivated from the SARS-CoV genome, the physiochemical properties such as the molecular weight, isoelectric point and extinction coefficient of each protein were calculated. The transmembrane segments and subeellular localization (SubLoeation) prediction and conserved protein motifs search against database were employed to analyze the function of SARS-CoV proteins. Also, the homology protein sequence alignment and evolutionary distance matrix calculation between SARS-CoV associated proteins and the corresponding proteins of other coronaviruses were employed to identify the classification and phylogenetic relationship between SARS-CoV and other coronaviruses. The results showed that SARS-CoV is a novel coronavirus which is different from any of the three previously known groups of coronviruses, but it is closer to BoCoV and MHV than to other coronaviruses. This study is in aid of experimental determination of SARS-CoV proteomics and the development of antiviral vaccine.     

Phylogeny of SARS-CoV as inferred from complete genome comparison

SARS-CoV, as the pathogeny of severe acute respi-ratory syndrome (SARS), seems to be the first coronavirus that is lethal to humans. Coronavirus (family Coronaviri-dae, genus Coronavirus) is an enveloped, single-stranded plus sense RNA virus whose genome has approximately 30 kb size. Whereas coronaviruses may cause severe dis-ease in animals, coronaviruses human strains only cause mild diseases until SARS-CoV was discovered. To date, SARS-CoV genomes from 12 isolates have been comp…     

Molecular phylogeny of coronaviruses including human SARS-CoV

The outbreak of SARS sets an urgent task to reveal the origin of human SARS-CoV, i.e. its relation to other known species of coronavirus, and to trace the genetic variation in the spreading process of SARS. Partial answer to the problem may be obtained from phylogenetic analy-sis of available genomes. We call a phylogenetic tree of different species of coronavirus including the human SARS-Cov a CoV Tree and that of different isolates of SARS-CoV a SARS Tree. CoV trees have been c…     

Genome sequence variation analysis of two SARS coronavirus isolates after passage in Vero cell culture

SARS coronavirus is an RNA virus whose replication is error-prone, which provides possibility for escape of host defenses, and even leads to evolution of new viral strains during the passage or the transmission. Lots of variations have been detected among different SARS-CoV strains. And a study on these variations is helpful for development of efficient vaccine. Moreover, the test of nucleic acid characterization and genetic stability of SARS-CoV is important in the research of inactivated vaccine. The whole genome sequences of two SARS coronavirus strains after passage in Vero cell culture were determined and were compared with those of early passages, respectively. Results showed that both SAPS coronavirus strains have high genetic stability, although nearly 10 generations were passed. Four nucleotide variations were observed between the second passage and the llth passage of Sinol strain for identification of SARS inactivated vaccine. Moreover, only one nucleotide was different between the third passage and the 10th passage of Sino3 strain for SARS inactivated vaccine. Therefore, this study suggested it was possible to develop inactivated vaccine against SARS-CoV in the future.     

Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus

Spike (S) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (SARS), associate with cellular receptors to mediate infection of their target cells. Here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ACE2), isolated from SARS coronavirus (SARS-CoV)-permissive Vero E6 cells, that efficiently binds the S1 domain of the SARS-CoV S protein. We found that a soluble form of ACE2, but not of the related enzyme ACE1, blocked association of the S1 domain with Vero E6 cells. 293T cells transfected with ACE2, but not those transfected with human immunodeficiency virus-1 receptors, formed multinucleated syncytia with cells expressing S protein. Furthermore, SARS-CoV replicated efficiently on ACE2-transfected but not mock-transfected 293T cells. Finally, anti-ACE2 but not anti-ACE1 antibody blocked viral replication on Vero E6 cells. Together our data indicate that ACE2 is a functional receptor for SARS-CoV.     

SARS冠状病毒的起源和进化初探

发展了一种研究基因序列进化的随机替代模型,根据一组从某个最近共同祖先演化而来的若干序列,可以预测此共同祖先序列,并计算随机替代概率矩阵,确定了从祖先序列的各个碱基到进化序列的各个碱基之间的替代概率。将这一模型应用于分析包括SARS冠状病毒在内的4种冠状病毒全基因组的演化规律,确定了在若干较保守的编码蛋白基因序列中同义替代位点的最近共同祖先序列以及分歧进化后的累计同义替代数目。结果表明,SARS病毒和其他几种已知的冠状病毒具有相当的进化历程,但存在不同的进化途径,支持了SARS病毒在造成此次大规模感染人体之前已经历了较长的进化历程的猜测。     

A Coordination Model of Gene Sequences for SARS-CoV and its Receptor

IntroductionFrom the emergence of SARS CoV, manystudies havebeen done on its biological medicine aspects, such aspathogeny characteristics, mechanisms of causing disease,clinic diagnosis and treatment, bacterin development andthe spreading rules. At the level of molecule biology,studies have been done on its genome sequences characteristics, structures and functions of the translatedproteins, and the evolution relationships in sequences[1 5].The caus…     

SARS相关冠状病毒刺突糖蛋白的研究(综述)

概述了SARS—CoV的S蛋白的结构及功能相关研究。严重急性呼吸综合症相关的冠状病毒(SARS-CoV)引起2003年我国南方非典型肺炎爆发流行，波及多个国家和地区。目前全球许多学对SARS-CoV进行了广泛的研究，发现S蛋白是病毒表面的主要蛋白，它构成冠状病毒科特征性的冠状样结构，在严重急性呼吸综合症的发病机制起着关键性作用，可介导表达相关受体的宿主细胞感染。现已鉴定出SARS-CoV的S蛋白相关受体，同时它在抗病毒感染中是一个关键靶蛋白。