酶法制备明胶的新工艺研究

阐述了一种酶法制备明胶的新工艺，并对酶用量、酶解温度、酶解时间、溶胶温度、溶胶初始pH值等参数进行了优化。同时，还测定了产品的理化性质。研究表明新工艺生产周期短(7～10d)，环境污染小，产率高。     

泥土芽孢杆菌YMTC1049菌株高温蛋白酶产酶条件的优化

 报道高温蛋白酶产生菌YMTC1049(Geobacillus sp.)产酶条件的优化过程.在基础培养基中分别添加葡萄糖、麦芽糖、酵母提取物、蛋白胨、胰蛋白胨、酪蛋白、聚胨等7种营养成分,获得对酶活影响较大的碳、氮源.用多因素正交试验设计考察了pH、酪蛋白、葡萄糖和温度对酶活的影响水平,菌株在优化发酵条件下培养24h时,上清液蛋白酶活力达312U/(mL·min).     

不同pH 条件下鳜鱼消化道蛋白酶活性研究

初步研究了不同pH条件下鳜鱼(Sinipercachuatsi)消化道不同部位蛋白酶活性的差异.结果表明鳜鱼消化道不同部位蛋白酶的活性存在较大差异,最适pH也不相同.消化道不同部位蛋白酶的活性强弱顺序为:幽门盲囊>胃>肠前段>肠后段.幽门盲囊的蛋白酶活性最强,明显高于胃、肠前段和肠后段.蛋白酶活性在幽门盲囊的最适pH为8 0～10 4,且在偏碱性条件下有较强的活性;在胃部的最适pH约为2 8,且在偏酸性的条件下活性较高;在肠前段和肠后段的最适pH为9 8.     

嗜热蛋白酶的研究与应用

介绍了国内外关于嗜热蛋白酶的研究成果与应用现状，包括产生嗜热蛋白酶的微生物种类，嗜热蛋白酶的特点、稳定机制及基因的克隆策略，嗜热蛋白酶的生产、提取、纯化以及应用．     

Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.     

Improved elastase production by Bacillus sp. EL31410—further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K(2)HPO(4), MgSO(4).7H(2)O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K(2)HPO(4) 0.206 g/100 ml and MgSO(4).7H(2)O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.     

培养条件对康宁木霉产酶的影响

通过对康宁木霉纤维素酶（C1酶和Cx酶）、酸性蛋白酶活力的比较,筛选出了康宁木霉产酶的最适培养条件.康宁木霉在m（麸皮）∶m（秸秆）=2∶8,氮源为w=1%的NH4NO3,含水量为65%,起始pH值为6.5的条件下培养72h,C1酶活力最高,为191.3U/g;在m（麸皮）∶m（秸秆）=6∶4,氮源为w=1%的（NH4）2SO4,含水量为65%,起始pH值为7.5的条件下培养48h,Cx酶活力最高,为2006.5U/g;在m（麸皮）∶m（秸秆）=8∶2,氮源为w=1%的（NH4）2CO3,含水量为65%,起始pH值为4.5的条件下培养96h,酸性蛋白酶活力最高,为1859.6U/g.     

基于生物信息学与分子结构的SARS冠状病毒主蛋白酶(SARS CoV Mpro)多肽类抑制剂研究

用冠状病毒基因解析软件系统ZCURVE_CoY2．0，从SARS冠状病毒（TOR2，NC_004718）的RNA基因序列出发，搜索出含有SARS冠状病毒主蛋白酶（SARS CoV M^pro）真实剪切位点的11个八肽，运用分子叠合和分子对接的方法，对11个八肽进行了分析，证明这11个八肽有相似的活性，而且11个八肽中，op4的活性最高．分析认为，op4有望成为抗SARS药物的理想前体．     

枯草芽孢杆菌YYW-1蛋白酶缺陷株诱变与筛选

从羽毛废弃物中筛选出一株高效降解羽毛、产角蛋白酶和蛋白酶的革兰氏阳性芽孢杆菌,经鉴定为枯草芽孢杆菌(Bacillus sublitis),命名为B.sublitisYYW-1。利用紫外线对菌株YYW-1进行诱变,筛选蛋白酶缺陷株,获得蛋白酶活性丧失的诱变株YYW-1-5,其蛋白酶活性残余22.7%,而角蛋白酶活性残余94.7%,没有明显的变化。     

量子点-菠萝蛋白酶与纤维蛋白原的相互作用

用量子点标记菠萝蛋白酶， 以此为荧光探针， 监测其与纤维蛋白原的反应过程. 实验结果表明， 随着反应时间的增加， 荧光光谱逐渐蓝移， 荧光强度也逐渐下降.