四膜虫有性生殖过程中特异表达基因TCD3的序列分析

含有CHROMO(chromatin organization modifier)结构域的蛋白参与了染色质结构的调节.本研究从嗜热四膜虫(Tetrahymena thermophila)全基因数据库(www.ciliate.org)和基因表达数据库(tged.ihb.ac.cn)中筛选到有性生殖特异表达TCD3(Tetrahymena chromo domain-containing protein 3)基因.该基因ORF为1 005bp,大核染色体中基因全长1 241bp,含有两个内含子.5′-RACE和3′-RACE表明5′端非编码区(5′-UTR)长度为40bp,3′端非编码区(3′-UTR)长度为90bp.TCD3基因预测编码334个氨基酸构成,含有一个CHROMO结构域.CHROMO结构域聚类分析表明四膜虫Tcd3蛋白可能与线虫CeCDP功能类似,参与异染色质的形成和DNA序列删除.     

Krull整环中w-理想的直和

主要给出关于Dedekind整环的两个经典结果在Krull整环上的体现.利用w-算子理论,证明了若R是Krull整环,A、B是R的非零理想,则AwBw■R(AB)w·进一步地,结合模的外幂的相关结果,证明了若R是Krull整环,I1,…,Im、J1,…,Jn是R的非零理想,则(I1)w…(Im)w■(J1)w…(Jn)w当且仅当m=n,且存在x∈K-0,使得(I1…In)w=x(J1…Jn)w.     

Kaplansky变换在高阶上的推广

给出了高阶Kaplansky变换的定义,并且刻画了高阶Kaplansky变换的基本性质.同时,对v-凝聚整环上的高阶Kaplansky变换进行了系统的研究,证明了若R是v-凝聚整环(分别地,Mori整环),则Ω(s)也是v-凝聚整环(分别地,Mori整环).     

热传导方程的一类区域分解差分算法

把求解区域分成若干个子域,在不同子域中采用不同的计算步长,对方程的紧差分格式在特殊情形下采用区域分解法,并给出相应的先验误差估计式。     

图书馆与大学生知识领域拓宽能力的培养

阐述了大学生知识领域拓宽的必要性,探讨了图书馆在大学生知识领域拓宽中的作用,提出了高校图书馆促进大学生知识领域拓宽的措施.     

双向模糊综合评判模型及应用

目的解决贸易谈判中的价格评估,寻找双方都能接受的价格区间。方法根据实际问题,建立一个商业谈判中双向模糊综合评判模型,并运用这个模型解决了服装谈判中买卖双方初步讨价还价的双向模糊综合评判问题。结果建立起了谈判模型,并利用模型分析了服装谈判。结论这个模型是可行的,并符合双方预期。     

单片机总线上多时钟域下数据传递的可靠性研究

对单片机总线上多时钟域下数据传递的可靠性进行研究，阐述了使用寄存器锁存电路、多级锁存电路2种方法解决多时钟域下数据传递可靠性时存在的问题，提出了采用4级锁存并判断跳变沿的方式能确保多时钟域下数据可靠传递的方法，具有很高的应用价值．     

系数优化在改善时间步进算法稳定性中的应用

隐式时间步进算法在求解二维物体的散射时,随着时间的推移,也会出现后时不稳定性的问题.文章在先五步后三步平均公式的基础上利用误差公式对系数进行筛选,改善了原公式在隐式时间步进算法中计算二维物体时产生的后时震荡情况；采用基于时域电场积分方程的隐式时间步进算法,分别计算了TM高斯脉冲入射到无限长导体圆柱和导体方柱时的导体表面...     

Prediction of sites under adaptive evolution in flavin-containing monooxygenases: Selection pattern revisited

Flavin-containing monooxygenase (FMO), like cytochrome P450 (CYP), is a monooxygenase that uses the reducing equivalents of NADPH to reduce one atom of molecular oxygen to water, while the other atom is used to oxidize the substrate. Recently, it was shown that some CYP isoforms have been subject to positive selection. However, it is unknown whether the highly conserved phase I detoxification enzyme, FMO, has undergone similar positive Darwinian selection. We used maximum-likelihood models of codon substitution, evolutionary fingerprinting, and cross species comparison to investigate the occurrence of adaptive evolution in FMO sequences. We used recent genomic data from a range of species, including vertebrates and invertebrates. We present the evidence for the occurrence of adaptive evolution in mammalian FMO 3, 4, 5, and fugu FMOs but not in mammalian FMO 1, FMO 2, frog FMOs, other fish FMOs and invertebrate FMOs. The sites under adaptive evolution were significantly associated with the insertion domain in mammalian FMO 5. We identified specific amino acid sites in FMOs 3–5 that are likely targets for selection based on the patterns of parallel amino acid change. The most likely role of adaptive evolution is the repair of mutations that permitted optimal NADP+ binding and improved catalytic efficiency. The occurrence of positive selection during the evolution of phase I detoxification enzymes such as FMOs 3–5 and fugu FMO suggests the occurrence of both high selection pressure acting on species within their unique habitats and significant changes in intensity and direction (forms of xenobiotics and drugs) resulting from changes in microhabitat and food.     

Amyloid precursor protein and its homologues: a family of proteolysis-dependent receptors

The Alzheimer’s amyloid precursor protein (APP) belongs to a conserved gene family that also includes the mammalian APLP1 and APLP2, the Drosophila APPL, and the C. elegans APL-1. The biological function of APP is still not fully clear. However, it is known that the APP family proteins have redundant and partly overlapping functions, which demonstrates the importance of studying all APP family members to gain a more complete picture. When APP was first cloned, it was speculated that it could function as a receptor. This theory has been further substantiated by studies showing that APP and its homologues bind both extracellular ligands and intracellular adaptor proteins. The APP family proteins undergo regulated intramembrane proteolysis (RIP), generating secreted and cytoplasmic fragments that have been ascribed different functions. In this review, we will discuss the APP family with focus on biological functions, binding partners, and regulated processing.