Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

Joint Strength with Soldering of Al2O3 Ceramics After Ni-P Chemical Plating

Ni-P alloy was chemically plated on Al2O3 ceramics to produce uniform alloy coatings at temperatures below 70~C. Cu metal was electroplated onto the Ni-P coating to facilitate the soldering and shorten the chemical plating time. Then, the electroplated ceramic specimens were soldered with 60 wt.% Sn-40 wt.% Pb solder in active colophony. The highest shear strength was acquired after the heat treatment at 170℃ for 15 min. The joint fractures mostly propagated along the interface between the ceramics and the Ni-P coating, with some fracture in both the ceramics and the Ni-P coating near the interface and some along the interface between the Cu and Ni-P coatings. The results show that ceramic surface roughness and the chemical plating parameters influence the coating quality, and that suitable heat treatment before the soldering also improves the adhesion between the ceramics and Ni-P coatings, thus strengthening the joints.     

用数据选择器和译码器的组合优化逻辑设计

介绍一种用数据选择器和译码器的组合优化逻辑设计的方法.该方法可减少数据选择器芯片及门电路使用的数量.文中给出几个实例.     

汽车碰撞事故的速度分析

通过对交通事故中汽车的碰撞问题的简化 ,运用动量守恒定理和能量守恒定律着重研究了正面碰撞、追尾碰撞和侧面碰撞三种情况下 ,汽车碰撞前动量和速度的求解方法 ,为交通事故模拟和再现提供了一种更为实用的方法 ,并为科学处理交通事故、明确事故责任提供了重要参考。     

300 km·h-1电动车组制动参数研究

在我国研制的先锋号电动车组制动系统的基础上 ,借鉴国外高速列车的经验 ,对自行研制 30 0km·h-1高速动车组制动系统的重要参数进行了研究 ,并提出了可供运用的设计值 .通过对先锋号动车组和 30 0km·h-1高速动车组制动参数的比较分析 ,指出先锋号制动系统的模式比较适合于 30 0km·h-1动车组 .     

关于W变换性质的进一步讨论

根据W变换和Delta函数的定义，讨论了函数f(x)的线性函数af(x) b的W变换和函数Ⅱi-1^n，(x)的W变换问题，并推广了W变换的线性性质，得到了几个进一步的结果．     

断裂带作为油气散失通道的输导能力

通过断裂散失的油气量主要与断裂带的输导能力、实际进入断裂带的油气量、断裂通道的长度、散失时间和储层分流系数等参数有关。断裂带输导能力的主要因素是断裂带的宽度和储层渗透率。利用断裂散失模型计算了不同规模的断裂带作为油气散失通道的输导能力。计算结果表明 ,大型断裂带输导油气的能力远大于储层输导油气的能力。断裂活动期进入“通天”大断裂带的油气主要趋于散失 ;小断裂由于储层的分流作用 ,即使在活动期 ,它作为油气散失通道的作用也是有限的 ,其油气的散失量可以忽略不计。     

有界随机变量的次高斯性

通过建立一些重要的不等式，研究有界随机变量的性质，得出了重要结果：数学期望为零的有界随机变量是次高斯的，并得出参数τ的精确估计。最后应用这些结果构造一个非对称的次高斯变量，并研究了某些随机三角级数的性质，得到了很好的结果。     

大兴井田4#煤层砂岩砾岩体特征及成因分析

运用沉积学理论,通过对铁法煤田大兴井田4#煤层中发育的砂岩、砾岩体的研究,指出它们均为河道沉积产物,并从中识别出了聚煤早期河道正常沉积、聚煤期后河道水携物与坍塌物混积和河道泥石流沉积三种成因类型,论述了它们各自的岩相和空间发育特征,这将有益于揭示其平面分布规律,为进一步指导采场的科学设计和修订提供了基础.     

飞行模拟器六自由度运动系统的关键技术及研究现状

作为飞行模拟器载体的并联六自由度运动系统是飞行模拟器非常重要的组成部分。其本质是由数字计算机实时控制并能提供俯仰、滚转、偏航、升降、纵向和侧向平移的六自由度瞬时过载仿真设备。本文从研制者的角度出发，评述了研制高性能飞行模拟器六自由度运动系统所需的关键技术及其国内外的研究进展。结合样机设计与制造的实践经验，提出亟需解决的若干理论与技术问题，以及解决这些问题的可行途径。