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1.
将Alyssum maritimum(L.)Lain.的种子灭菌后,分别接人培养基MS+6-BA1.0mg/L+NAA0.2mg/L、MS+6-BA1.5mg/L+NAA0.2mg/L、MS+6-BA2.0mg/L+NAA0.2mg/L、MS+6-BA2.5mg/L+NAA0.2mg/L中,得到无菌苗.取20d的无菌苗叶子切成3minx2mm的小块,接人同样的培养基中诱导芽,在MS+6-BA2.5mg/L+NAA0.2mg/L培养基上分化得到芽,将芽转入不同NAA浓度MS培养基上生根,实验表明,A.maritimum(L.)Lam.芽诱导最适合的培养基是MS+6-BA2,5mg/L+NAA0.2mg/L.  相似文献   

2.
以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方:(1)芽诱导培养基:MS+BA0.6mg/L+NAA0.2mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(2)增殖培养基:Ms+BA0.8mg/L+NAA0.05mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(3)生根培养基:MS+BA0.05mg/L+NAA0.8mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8.观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d.  相似文献   

3.
兰州百合的组织培养   总被引:4,自引:0,他引:4  
以兰州百合鳞片作为外植体进行组织培养,分别对其进行芽诱导、增殖、生根培养,得到了兰州百合鳞片组织培养的最佳培养基配方:(1)芽诱导培养基:MS+BA0.6mg/L+NAA0.2mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(2)增殖培养基:Ms+BA0.8mg/L+NAA0.05mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8;(3)生根培养基:MS+BA0.05mg/L+NAA0.8mg/L+琼脂7.3g/L+蔗糖30g/L,pH5.8.观察与分析结果表明,外植体在前两种培养基上的繁殖速率较前人快约15d,第3种培养基与前人的相当,比其快2d.  相似文献   

4.
对海滨锦葵组织培养再生体系进行了研究,结果表明:1.海滨锦葵茎段外植体较好的愈伤组织诱导培养基组合为MS+KT1.5mg/L+IAA2.0mg/L或MS+6-BA4.0mg/L+IBA0.2mg/L+Inositol50mg/L.2.海滨锦葵愈伤诱导不定芽的较好培养基组合为MS+NAA2.0mg/L+2iP5.0mg/L和MS+NAA0.5mg/L+2iP5.0mg/L.3.海滨锦葵腋芽分化不定芽的较好培养基组合为改良MS(含2倍有机营养)+KT0.4mg/L+IAA1.0mg/L.MS基本培养基中适当提高有机营养的含量对腋芽的增殖有促进作用,实验中以有机营养的含量为基本培养基2倍时效果较好.4.不定芽在1/ZMS的培养基中附加IBA1.0mg/L牛根最好.  相似文献   

5.
多因子正交试验对长春花离体培养条件的筛选   总被引:3,自引:0,他引:3  
应用正交设计法考察了植物生长物质、蔗糖对长春花愈伤组织诱导、丛生芽诱导和生根培养的影响。最佳愈伤组织诱导培养基为MS+2,4-D0.8mg/L NAA 1mg/L ZT 0.2mg/L。丛生芽诱导最佳培养基为MS+6-BA0.2mg L NAA 0.3mg/L+蔗糖30g/L,最佳生根培养基为1/2MS+NAA 0.2mg/L IBA 0.2mg/L 蔗糖30mg/L。  相似文献   

6.
贴梗海棠组培快繁技术研究   总被引:1,自引:0,他引:1  
袁秀云  张仙云  马杰 《河南科学》2009,27(2):175-177
对贴梗海棠的外植体进行了愈伤组织和植株再生体系研究.结果表明,带芽茎段为最适外植体;诱导芽的最适培养基为Ms+6-BA1.0mg/L+NAA0.2mg/L芽生长培养基为MS+6-BA2.0mg/L+NAA0.2mg/L;在1/2MS+NAA0.3mg/L培养基上,生根率为90%.  相似文献   

7.
以猪笼草的幼嫩茎段为外植体进行离体组织培养及植株再生,研究结果表明:以液体培养基1/2MS+6-BA1.0mg/I.+NAA0.1mg/L对芽的初始诱导以及固体培养基1/2MS+6-BA2.0mg/L+NAA0.1mg/L对不定芽的增殖效果最好,增殖率达419%;生根培养基以1/4MS+IBA0.5mg/L+活性碳(0.01%)为最佳,生根率可达100%.  相似文献   

8.
兰州百合和野百合组织培养及快速繁殖研究   总被引:42,自引:1,他引:42  
以兰州百合(Lilium davidii Var.Unicolor (Hoog)Cotton)和野百合(Lilium brownii F.E.Brown ex Miellez) 鳞茎及叶片为外植体获得再生植株,并建立起快速无性繁殖系。兰州百合鳞茎的最佳诱导培养基为MS+0.6-1.0mg/LBA+0.2mg/LNAA;芽增殖培养基选MS+0.5-0.6mg/LBA 0.1mg/L NAA;生根培养基为1/2MS+0.2mg/l IAA 0.1%活性炭。野百合鳞茎及叶的最佳诱导培养基分别为MS+0.1-0.5mg/LBA 0.1-0.2mg/L NAA或0.5mg/L IAA和MS+0.7mg/L BA 0.07mg/L NAA;鳞茎诱导的芽的增殖培养基为MS+0.2-0.4mg/L BA 0.1mg/L NAA;生根培养基为1/2MS+0.15mg/L NAA 0.1%活性炭。  相似文献   

9.
选取经资源调查及品质分析后获得的优良植株的顶芽和腋芽为材料,诱导腋芽的萌发和不定芽增殖生根.结果表明:从3月下旬到5月上旬是菠萝蜜组织培养大田取材的最佳时间,该时期接种材料污染率(〈50%)及褐变率(〈5%)均较低;启动培养的培养基最佳组合是MS+6-BA2.0mg/L+KT1.0mg/L+GA。0.5mg/L+蔗糖20g/L,腋芽诱导率为44.6%,KT含量较高(1.0mg/L)有利于腋芽的诱导,诱导率平均为(40%);不定芽增殖培养的培养基最佳组合是MS+6-BA1.5mg/L+KT0.1mg/L+GA。0.5mg/L+蔗糖40g/L,增殖率为2.61,蔗糖含量较高(40g/L)有利于不定芽的增殖,增殖率平均为2.34;生根培养以1/2MS+IBA1.5mg/L+NAA0.2mg/L最好,接种8d开始生根,生根率15.8%,15d根长3~4cm,平均根数3~4条.  相似文献   

10.
蒲苇愈伤组织的诱导和再生体系的建立   总被引:1,自引:1,他引:0  
以蒲苇成熟种子为外植体,MS为基本培养基并附加不同激素组合,筛选出诱导蒲苇愈伤组织产生和分化的最佳培养基,成功建立蒲苇的再生体系.试验结果表明:蒲苇种子最佳灭菌方式是将种子灭菌前用无菌水浸泡24h左右,然后用75%酒精消毒20s,再用0.1%(W/V)氯化汞消毒灭菌10min.愈伤组织诱导的最佳培养基为MS+2,4-D3.0mg/L+6-BA0.1mg/L;愈伤组织分化的最佳培养基为MS+6-BA 2.0mg/L+NAA 0.1mg/L分化继代的最佳培养基为MS+6-BA 1.5mg/L+NAA 0.2mg/L;生根最佳培养基配方为1/2MS+NAA 0.8mg/L+6-BA 0.2mg/L+(0.02%)AC.  相似文献   

11.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

12.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

13.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

14.
Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to  相似文献   

15.
Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications (up to 574.8 km/ h test speeds or 380 km/h commercial speeds) in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.  相似文献   

16.
17.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

18.
Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.  相似文献   

19.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

20.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

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