| 兰州百合的组织培养 |
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| 引用本文： | 韩华丽,郭成金.兰州百合的组织培养[J].天津师范大学学报(自然科学版),2009,29(3):62-65. |
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| 作者姓名： | 韩华丽 郭成金 |
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| 作者单位： | 天津师范大学化学与生命科学学院,天津,300387 |
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| 摘 要： | 以兰州百合鳞片作为外植体进行组织培养，分别对其进行芽诱导、增殖、生根培养，得到了兰州百合鳞片组织培养的最佳培养基配方：（1）芽诱导培养基：MS＋BA0．6mg／L＋NAA0．2mg／L＋琼脂7．3g／L＋蔗糖30g／L，pH5．8；（2）增殖培养基：Ms＋BA0．8mg／L＋NAA0．05mg／L＋琼脂7．3g／L＋蔗糖30g／L，pH5．8；（3）生根培养基：MS＋BA0．05mg／L＋NAA0．8mg／L＋琼脂7．3g／L＋蔗糖30g／L，pH5．8．观察与分析结果表明，外植体在前两种培养基上的繁殖速率较前人快约15d，第3种培养基与前人的相当，比其快2d．
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| 关 键 词： | 兰州百合 鳞片 组织培养 |
The tissue culture method of Lanzhou Lily |
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| HAN Huali,GUO Chengjin.The tissue culture method of Lanzhou Lily[J].Journal of Tianjin Normal University(Natural Science Edition),2009,29(3):62-65. |
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| Authors: | HAN Huali GUO Chengjin |
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| Institution: | (College of Chemistry and Life Science. Tianjin Normal University, Tianjin 300387, China) |
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| Abstract: | The optimal culture mediums were obtained by tissue culture method from Induction of bud, culture of multiplica-tion and culture of root of taking Lanzhou Lily as a exophyte. They were: (1) the culture medium of bud induction: MS+BA 0. 6 mg/L+NAA 0. 2 mg/L+agar 7. 3 g/L+sucrose 30 g/L, pH 5.8. (2) the culture medium of multiplication:MS+BA 0. 8 mg/L+NAA 0.05mg/L+agar 7.3 g/L+sucrose 30 g/L,pH 5. 8. (3) the culture medium o{ rooting cul-ture: MS+BA 0.05 mg/L+NAA 0.8 mg/L+agar 7. 3 g/L+sucrose 30 g/L,pH 5.8. Compared with existing ones, the multiplication rate of the former two ones were faster 15 days, the third culture medium was faster 2 days. |
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| Keywords: | Lanzhou Lily scales tissue culture |
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