菠萝蜜的组织培养

选取经资源调查及品质分析后获得的优良植株的顶芽和腋芽为材料，诱导腋芽的萌发和不定芽增殖生根．结果表明：从3月下旬到5月上旬是菠萝蜜组织培养大田取材的最佳时间，该时期接种材料污染率（〈50％）及褐变率（〈5％）均较低；启动培养的培养基最佳组合是MS＋6-BA2．0mg／L＋KT1．0mg／L＋GA。0．5mg／L＋蔗糖20g／L，腋芽诱导率为44．6％，KT含量较高（1．0mg／L）有利于腋芽的诱导，诱导率平均为（40％）；不定芽增殖培养的培养基最佳组合是MS＋6-BA1．5mg／L＋KT0．1mg／L＋GA。0．5mg／L＋蔗糖40g／L，增殖率为2．61，蔗糖含量较高（40g／L）有利于不定芽的增殖，增殖率平均为2．34；生根培养以1／2MS＋IBA1．5mg／L＋NAA0．2mg／L最好，接种8d开始生根，生根率15．8％，15d根长3～4cm，平均根数3～4条．

Study on Tissue Culture of Jackfruit

An in vitro cultrue method for clonal propagation of a high quality strain of jackruit has been achieved,using shoot buds as explants.Results showed that,selection of explant collecting seasons was critical in preventing contamination and brownization,form late March to early May which was the best seasons.Addition of 2.0 mg/L 6-BA and 1.0 mg/L KT and 0.5 mg/L GA3 to MS nutrient medium induced maximum number of shoot buds(44.6%),higher KT concentration helped to induce high buds(40%).These shoots proliferated best in MS medium added BA1.5 mg/L,KT0.1 mg/L and GA3 0.5mg/L,with a rate of 2.61.High concentrations of sugar(40 g/L) helped to shoot proliferation,with an average rate of 2.43.For rooting,1/2MS+IBA1.5mg/L+NAA0.2mg/L 1.5 was the suitable medium.Rooting began 8d after implantation,with a rooting rate of 15.8%.15d later,root length was 3 to 4cm,average root number was 3-4.