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1.
福建省山樱花的组织培养及植株再生   总被引:5,自引:0,他引:5  
以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽 。在1/4MS+BA1.0mg/L IBA0.1mg/L的培养基上,其丛生芽诱导率达87.5%;在继代培养中选择MS+BA1.0mg/L IBA0.1mg/L GA30.3mg/L或KT1.0mg/L NAA0.1mg/L BA30.3mg/L培养基,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果,当芽伸长在3-4cm时,切下置于生根培养基1/2MS+NAA1.0mg/L IBA1.0mg/L BA0.75mg/L中,生根率达90%以上,移栽成活率达95%。  相似文献   

2.
兰州百合和野百合组织培养及快速繁殖研究   总被引:42,自引:1,他引:42  
以兰州百合(Lilium davidii Var.Unicolor (Hoog)Cotton)和野百合(Lilium brownii F.E.Brown ex Miellez) 鳞茎及叶片为外植体获得再生植株,并建立起快速无性繁殖系。兰州百合鳞茎的最佳诱导培养基为MS+0.6-1.0mg/LBA+0.2mg/LNAA;芽增殖培养基选MS+0.5-0.6mg/LBA 0.1mg/L NAA;生根培养基为1/2MS+0.2mg/l IAA 0.1%活性炭。野百合鳞茎及叶的最佳诱导培养基分别为MS+0.1-0.5mg/LBA 0.1-0.2mg/L NAA或0.5mg/L IAA和MS+0.7mg/L BA 0.07mg/L NAA;鳞茎诱导的芽的增殖培养基为MS+0.2-0.4mg/L BA 0.1mg/L NAA;生根培养基为1/2MS+0.15mg/L NAA 0.1%活性炭。  相似文献   

3.
卡特丽亚兰的组织培养与快速繁殖的研究   总被引:6,自引:1,他引:5  
作者以卡特丽亚兰开花植株茎尖和试管苗茎段或叶切段为外植体,成功地诱导形成原球茎并获得再生植株,筛选出了原球茎球诱导培养基:RE=60mg/L BA(5mg/L BA 0.5-1mg/L NAA或IBA)+0.5%-1%蔗糖;原球茎增殖培养基:MS(或KC)+5mg/L BA 0.1(0.5)mg/L NAA(或0.5mg/L 1BA) 0.2%蛋白胨和分化及育苗培养基;KC+0.3mg/L (0.5)NAA 10%香蕉匀汁+0.1%活性炭。BA在原球茎的产生、增殖分化中起着主导作用。  相似文献   

4.
蔓茎堇菜愈伤组织分化再生植株   总被引:3,自引:0,他引:3  
研究了蔓茎堇菜植株再生的培养条件。通过L9(3^4)正交试验筛选出诱导蔓茎堇菜愈伤组织的适合培养基为MS+2,4-D1.5+ZT1.0;4个激素组合即ZT2.0+NAA0.5、ZT2.0+IBA0.5、6-BA2.0和6-BA2.0+NAA0.5可诱导出蔓茎堇菜芽;诱导根分化与植株培养的适宜培养基为MS+NAA2.0+6-BA(或ZT)0.25或MS+(IBA+IAA)1.0+6-BA(或ZT)0.25。  相似文献   

5.
丽格海棠的组织培养与快速繁殖研究   总被引:9,自引:0,他引:9  
以丽格海棠的茎状、叶柄、叶片为外植体进行组织培养。最佳培养基:(1)诱导培养基MS+6-BA4.0mg/L(单位下同)+IBA1.0;(2)增殖培养基MS+6-BA0.5+IAA0.2;(3)生根培养基MS+NAA0.2.生根壮苗培养50d左右,经移栽,成活率达92%左右。  相似文献   

6.
以杜仲(EncommiaulmoidesOliver)当年生枝条的叶片、带芽茎段为外植体,基本培养基为MS,附加不同浓度的BA、KT和NAA.最佳芽诱导培养基为:MS KT1 0mg/L NAA0 1~0 5mg/L;MS BA2 0mg/L NAA0 1~0 5mg/L,最佳芽丛增殖培养基为:MS KT2 0mg/L NAA0 1~0 2mg/L;MS BA1 0~2 0mg/L NAA0 1~0 2mg/L;MS KT2 0mg/L IBA0 1~0 2mg/L;MS BA1 0~2 0mg/L IBA0 1~0 2mg/L,单芽增殖培养基:MS KT1 0mg/L NAA0 1mg/L,生根培养基:13MS KT0 1mg/L NAA0 5mg/L.  相似文献   

7.
以粉蕉吸芽为材料开展茎尖离体快速繁殖研究,结果表明:以MS+BA3-5mg/l(单位不同)+NAA0.2为诱导培养基可以获得不定芽;以MS+BA3.0+Ad10.0为继代培养基可以获得较高的不定芽增殖率且变异率极低,后期附加一定量的NAA有助于促根;生根培养以MS+IBA1.0-2.0+NAA0.1+Ac1为宜。  相似文献   

8.
以竹节秋海棠的幼嫩叶作外植体进行了组织培养 .结果表明:( 1)在不同激素组合的培养基上均能诱导产生丛生芽,在 MS+ BA2mg/L+ 2.4- D0.2mg/L中分化率高,芽质优;( 2) MS+ BA0.5mg/L+ NAA0.2mg/L和 MS+ BA0.5mg/L+ 2.4- D0.05mg/L培养基中有效苗获得率高,长势好;( 3)再生植株在 1/2MS+ IBA0.2mg/L+ NAA0.2mg/L培养基中的生根率达 96% .  相似文献   

9.
非洲菊的离体培养及其快速繁殖   总被引:12,自引:0,他引:12  
选用非洲菊(Gerbera jamesonii Bolus)4个黑蕊花品种进行组培快繁研究,结果表明,长0.5-1.0cm的嫩叶在MS+2.0mg/L 6-BA 0.1mg/L NAA培养基上12-21d均可诱导出芽,分化频率60%以上;继代培养时MS+0.3mg/L 6-BA 0.1mg/L NAA,MS 1.0mg/L 6-BA 0.1mg/L NAA ey MS 0.3mg/L 6-BA 0.1mg/L PP333 0.1mg/L NAA3种培养基交替使用,平均增殖系数可达5.1;生根培养基为MS+0.1ms/L PP333 0.1mg/L NAA,生根率达95%以上;生根试管苗移栽于泥炭土中15-20d可以成活,25d后可定植于大田,3-4个月即可开花,该研究为黑蕊花品种种苗产业化提供了快繁技术。  相似文献   

10.
多因子正交试验对长春花离体培养条件的筛选   总被引:3,自引:0,他引:3  
应用正交设计法考察了植物生长物质、蔗糖对长春花愈伤组织诱导、丛生芽诱导和生根培养的影响。最佳愈伤组织诱导培养基为MS+2,4-D0.8mg/L NAA 1mg/L ZT 0.2mg/L。丛生芽诱导最佳培养基为MS+6-BA0.2mg L NAA 0.3mg/L+蔗糖30g/L,最佳生根培养基为1/2MS+NAA 0.2mg/L IBA 0.2mg/L 蔗糖30mg/L。  相似文献   

11.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

12.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

13.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

14.
Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to  相似文献   

15.
Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications (up to 574.8 km/ h test speeds or 380 km/h commercial speeds) in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.  相似文献   

16.
17.
Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.  相似文献   

18.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

19.
Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with  相似文献   

20.
The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to  相似文献   

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