| 卡特丽亚兰的组织培养与快速繁殖的研究 |
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| 引用本文： | 丁兰,梁桂霞,傅华龙.卡特丽亚兰的组织培养与快速繁殖的研究[J].四川大学学报(自然科学版),2001,38(1):106-110. |
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| 作者姓名： | 丁兰 梁桂霞 傅华龙 |
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| 作者单位： | 1. 西北师范大学生物系,
2. 四川大学生物系, |
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| 摘 要： | 作者以卡特丽亚兰开花植株茎尖和试管苗茎段或叶切段为外植体，成功地诱导形成原球茎并获得再生植株，筛选出了原球茎球诱导培养基：RE＝60mg/L BA(5mg/L BA 0.5-1mg/L NAA或IBA）＋0．5％－1％蔗糖；原球茎增殖培养基：MS（或KC）＋5mg/L BA 0.1(0.5)mg/L NAA(或0.5mg/L 1BA) 0.2%蛋白胨和分化及育苗培养基；KC＋0．3mg/L (0.5)NAA 10%香蕉匀汁＋0．1％活性炭。BA在原球茎的产生、增殖分化中起着主导作用。
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| 关 键 词： | 卡特丽亚兰 原球茎 无性繁殖 组织培养 快速繁殖 兰花 |
| 文章编号： | 0490-6756(2001)01-0106-05 |
STUDY ON TISSUE CULTURE
AND RAPID PROPAGATION OF CATTLEYA IN VILRO |
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| DING Lang,NINAG Gui-xia,FU Hua-long.STUDY ON TISSUE CULTURE
AND RAPID PROPAGATION OF CATTLEYA IN VILRO[J].Journal of Sichuan University (Natural Science Edition),2001,38(1):106-110. |
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| Authors: | DING Lang NINAG Gui-xia FU Hua-long |
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| Institution: | DING Lang 1,NINAG Gui xia 1,FU Hua long 2 |
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| Abstract: | The clonal propagation of Cattleya was developed. PLB is obtained through culture of leaf segments and shoot-tips and shoot stem of Cattleya on KC solid medium with BA 6mg/L, NAA 0.5 % ～ lrng/L and sucrose 0.5 %～ 1%. proliferation of Protocorms is on MS or KC medium with BA 5rng/L and NAA 0.1～0.5mg/L or IBA 0.5mg/L and Peptone 0.2%. The medium as KC or MS with NAA 0.3 or 0.5rng/L and 10% banana extracts and 0.1% actived charcoal is better for plantlet growth. BA played a central role in the protocorm formation and differentiation. |
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| Keywords: | Cattleya protocorm clone |
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