中国河南西峡恐龙蛋化石中18SrDNA部分片段的克隆及序列…

本文从河南省西峡晚白垩世的一枚保存方式特殊的恐龙蛋化石中提取ＤＮＡ，经过＾３２Ｐ同位素标记和电泳分析，发现在蛋内腔的絮状物样品中确在ＤＮＡ片段存在。它们的大小约在１０００ｂｐ至几十ｂｐ之间，大多数片段在４００ｂｐ以下。用１８ＳｒＤＮＡ特异引物，以上述ＤＮＡ为模板进行了ＰＣＲ扩增，经电泳分析得到约２００ｂｐ的ＰＣＲ产物。经过连接、转化到大肠杆菌，最终筛选出６个阳性克隆。测序分析和同源性比较发现这６个     

河南西峡恐龙18srDNA片段质颖

按照ｒＤＮＡ的结构特点，将ＤＡ１８Ｓ１，７与其他８种生物的ｒＤＮＡ序列进行排列比较，结果表明这２上序列不是恐龙ｒＤＮＡ片段ＤＡ１８Ｓ７看来更接近一种植物ｒＤＮＡ片段，而ＤＡ１８Ｓ１与２种参与比较的真菌ｒＤＮＡ序列有很高的同源性，它们与脊椎动物ｒＤＮＡ的同源性分别只在６７．８％－７４．１％之间。     

马铃薯天冬氨酸蛋白酶抑制剂基因克隆和结构分析

以马铃薯基因组ＤＮＡ为模板并基于天冬氨酸蛋白酶抑制剂基因的ｃＤＮＡ序列所设计的两个引物，通过ＰＣＲ扩增得到６６６ｂｐ的天冬氨酸蛋白酶抑制剂基因编码区，并将此片段克隆到ｐＵＣ１８的ＳｍａＩ位点．序列分析结果表明该基因除去末端一终止密码子外其可译框架编码２２１个氨基酸残基，前导肽包括３２个氨基酸残基，故该抑制剂的成熟蛋白由１８９个氨基酸组成，第６７位的精氨酸为抑制胰蛋白酶的活性中心．与ｃＤＮＡ相比核苷酸的同源性为９４．３％，氨基酸的同源性为９０％．基因ＤＮＡ无内含子．     

河南西峡恐龙18srDNA片段质疑

按照ｒＤＮＡ的结构特点，将ＤＡ１８Ｓ１，７与其他８种生物的ｒＤＮＡ序列进行排列比较，结果表明这２个序列不是恐龙ｒＤＮＡ片段，ＤＡ１８Ｓ７看来更接近一种植物ｒＤＮＡ片段（与金虎尾的同源性达９４％以上），而ＤＡ１８Ｓ１与２种参与比较的真菌ｒＤＮＡ序列有很高的同源性（８８．３％和８９％），它们与脊椎动物ｒＤＮＡ的同源性分别只在６７．８％～７４．１％之间。     

牛α—s1酪蛋白基因3‘端DNA的PCR扩增,克隆及鉴定

本研究以牛血总ＤＮＡ为模板，用ＰＣＲ技术扩增牛α－ｓ１酪蛋白基因的５＇端３．６ｋｂＤＮＡ片段和３＇端１．５ｋｂＤＮＡ片段的调控区序列，得到了相应的特异性扩增片段．用Ｋｌｅｎｏｗ处理后，将两个扩增片段分别与经Ｓｍａｌ酶切的ｐＵＣ１８质粒载体用Ｔ４ＤＮＡ连接酶连接，转化大肠杆菌ＪＭ１０９．在含有Ｘ－ｇａｌ，ＩＰＴＧ和Ａｍｐ的选择培养基上培养．从白色菌落中提取质粒ＤＮＡ，进行限制酶切鉴定．结果得到一个插入有１．３ｋｂＤＮＡ片段的阳性克隆．对其进行部分序列分析表明，该片段为５＇端缺失约１７０ｂｐ的牛α－ｓ１酪蛋白基因３＇端及下游区序列．     

绵羊β—乳球蛋白基因调空序的分离,克隆及序列分析

根据绵羊β－乳球蛋白基因调控区ＤＮＡ序列设计了两个引物，以绵羊基因组ＤＮＡ为模板，用ＰＣＲ技术扩增，得约９００ｂｐ的ＤＮＡ片段插入到ｐＵＣ１８质粒的ＸｂａＩ／ＫｐｎＩ位点之间。     

盐藻磷酸甘油脱氢酶基因cDNA文库的构建

采用Ｌａｍｂｄａ ｇｔ１０载体构建了盐藻ｃＤＮＡ文库，文库大小为１０＾６／ｍＬ插入片段平均长度大于１ｋｂ。根据果蝇，兔，鼠编码其３－磷酸甘油脱氢酶基因的辅酶ＮＡＤ的结合功能区保守序列设计一对引物，大小分别为２１ｂｐ和１８ｂｐ。ＰＣＲ扩增得到与果蝇相同的２９０ｂｐ特异扩增片段，以该片段为探针，采用缺口平移系统标记原位杂交，从盐藻ｃＤＮＡ文库中获得３６个３－磷酸甘油脱氢酶基因的阳性克隆。     

人胰岛素原乳腺表达载体的构建

通过ＰＣＲ方法扩增了人胰岛素（ＨＩ）基因１．３４ｋｂ的ＤＮＡ片段并克隆于ｐＵＣ１８的ＳｍａⅠ位点内。经ＤＮＡ序列分析表明，该片段为ＨＩ基因的氨基酸编码区及３＇端调控区序列。同时，应用ＰＣＲ方法对牛α－乳白蛋白（ＢαＬＡ）基因进行了扩增，得到了０．８４ｋｂＤＮＡ扩增片段。将其克隆于去除了ＥｃｏＲＩ位点的ｐＵＣ１８ＳｍａＩ位点内，经内切酶分析和ＤＮＡ测序证明该片段是ＢαＬＡ基因５＇调控区序列。ＥｃｏＲ     

水稻共生菌DX01的分子鉴定及电击转化条件的初步探索

用ＰＣＲ的方法扩增了水稻表面共生菌ＤＸ０１的１６Ｓ ｒＤＮＡ片段,并进行了部分序列的测定和ＢＬＡＳＴ同源序列比较分析,结果表明,该序列３’端５５０ｂｐ与短小芽孢杆菌（Ｂａｃｉｌｌｕｓ ｐｕｍｉｌｕｓ）１６ Ｓ ｒＤＮＡ的同源性达９９％,５‘端６８０ｂｐ为９８％,故ＤＸ０１应为Ｂ．ｐｕｍｉｌｕｓ。对其电击法转化的部分条件进行了初步的探索,结果表明：当细菌处于Ｄ（６００）为０．９的生长期时,使用ＰＥＢ     

绵羊β-乳球蛋白基因调控序列的分离、克隆及序列分析

根据绵羊β－乳球蛋白基因调控区ＤＮＡ序列设计了两个引物，以绵羊基因组ＤＮＡ为模板，用ＰＣＲ技术扩增，得约９００ｈＰ的ＤＮＡ片段插入到ｐＵＣ１８质粒的ＸｂａＩ／ＫｐｎＩ位点之间．ＰＣＲ产物的克隆经双酶切、ＰＣＲ检测和序列分析证明是绵羊β－乳球蛋白基因的调控序列．序列分析结果表明该克隆片段与绵羊β－乳球蛋白基因相应ＤＮＡ序列相比有很高的一致性．研究结果为指导异源基因的表达和进一步利用乳腺特异表达的转基因动物生产医用蛋白提供了有效的调控序列．     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.