罗汉果性别相关的RAPD标记

应用随机扩增多态性DNA(RAPD)技术寻找与罗汉果性别相关的分子标记,筛选了130个10 bp的随机引物,发现有4个引物(S60、S90、S100、S343)能在雌、雄株DNA间扩增出5条差异性片段,大小在300~1 300 bp之间,表明这些特异带可被用来作为性别鉴别的特异性分子遗传标记。     

草鱼基因组随机扩增多态性引物及多态性位点的筛选

为了建立草鱼基因组DNA多态性分析的指标体系,应用RAPD技术,从180条10碱基随机引物中筛选出了31条能扩增出多态性DNA片段的引物。用这31条多态性引物共扩增出了327条重复性好、带型清晰、分辨率高的谱带。扩增产物的片段大小范围在400—2000bp之间。单一引物扩增条带为5—14条。用这些多态性引物在草鱼基因组DNA中检测到了93个多态性位点,并对这些多态性位点上的等位基因频率进行了统计分析。这31条多态性引物和所检测到的93个多态性位点初步为草鱼基因组的多态性分析提供了可靠的分析指标体系。     

福建省桫椤科植物的分子分类学研究

桫椤科科下等级设立长期以来存在一定的争议，以福建省内5种桫椤科植物刺桫椤（Alsophila spinulosa(Hook,)Tryon）、黑桫椤（Gymnosphaera podophylla(Hook.)Copel.）、针毛桫椤（G.metteniana (Hance)Tagawa）、粗桫椤（G.hancokii (Copel,)Ching）、牛姆林桫椤（G.niumulinensis Li,Chen et Deng）（新拟）为材料，采用改进的CTAB法获得了纯度较高，得率高，片段完整（片段大小均大于23kb）的基因组DNA，并运用RAPD技术对这5种桫椤进行了遗传多样性分析，从40个10－mer随机引物中筛选30个有效引物，产利用这30个有效引物共扩增出1073条DNA带，利用UPGA法以桫椤科的5个种的种间亲缘关系进行聚类分析，得出5个种的DNA分子分类系统图。结果表明，可以分为毛桫椤、棘桫椤和黑桫椤3组，平均遗传距离为0．61，符合属间的关系，从而确定了RAPD技术用于桫椤植物分子分类研究的可行性。结合前人在形态解剖学方面的工作，提出这5种桫椤科植物可以分为3个类群，即刺桫椤类、黑桫椤类和针毛桫椤类，并提供了3个类群的检索表。     

中国4个两性生殖卤虫品系的ISSR指纹分析

根据微卫星DNA序列设计了10种ISSR引物，对中国4个两性生殖卤虫品系的亲缘关系进行了分析．用10种ISSR引物对卤虫基因组DNA进行扩增，共获得188条清晰稳定的条带，75个基因座位，其中有99条多态性片段，53个多态性座位，片段长度在2000-150bp之间．聚类分析结果表明：3个内蒙品系卤虫亲缘关系较近，当遗传相似系数为0．5333时，明显把来自内蒙古和西藏的卤虫分为2个类群．     

不同生理状态下原生动物草丛土毛虫大核DNA的多态性比较

对草丛土毛虫（Territricha stramenticola）营养细胞大核DNA和休眠包囊大核DNA进行了RAPD比较分析．在所选用的32条随机引物中,2条没有扩增带,其余30条引物一共扩增出134条片段,以营养细胞大核DNA为模板扩增出54条片段,以休眠包囊大核DNA为模板扩增出80条片段,两者有20条共享片段,共享度为30％．结果表明：草丛土毛虫在形成包囊的过程中,大核DNA的结构可能发生了较大的变化,这些变化可能与其在包囊形成过程中的形态结构和代谢活动的改变以及休眠状态下生理活动的改变密切相关．     

桂花品种的ISSR-PCR分析

<正>利用ISSR—PCR方法对桂花的54个品种进行了基因组多态性分析,从70个ISSR引物中筛选出13个多态性引物用于正式扩增,共扩增出90条DNA片段,其中多态性DNA带79条,占总扩增片段的87.8％,平均每个引物扩增的DNA带的数目为6.92条。依据扩增结果,应用RAPD Distans软件进行Nei相似性系数和遗传距离计算,利用UPGMA法构建聚类树状图。结果表明:ISSR分析中产生了一些品种特有的指纹图谱,因此ISSR技术在桂花品种和品种群(品系)的鉴定和阐明遗传关系中有很大的应用潜力。利用DNA扩增结果进行聚类分析,把供试桂花的54个品种分为7个大类,并对品种进行了处理及种下品种群的遗传关系进行了探讨。     

桂花品种的ISSR-PCR分析

利用ISSR—PCR方法对桂花的54个品种进行了基因组多态性分析,从70个ISSR引物中筛选出13个多态性引物用于正式扩增,共扩增出90条DNA片段,其中多态性DNA带79条,占总扩增片段的87.8％,平均每个引物扩增的DNA带的数目为6.92条。依据扩增结果,应用RAPD Distans软件进行Nei相似性系数和遗传距离计算,利用UPGMA法构建聚类树状图。结果表明:ISSR分析中产生了一些品种特有的指纹图谱,因此ISSR技术在桂花品种和品种群(品系)的鉴定和阐明遗传关系中有很大的应用潜力。利用DNA扩增结果进行聚类分析,把供试桂花的54个品种分为7个大类,并对品种进行了处理及种下品种群的遗传关系进行了探讨。     

蜡梅品种的RAPD分析

从200个随机引物中筛选出16个多态性稳定的引物,对38个蜡梅品种进行遗传多样性和亲缘关系RAPD分析.共扩增出154条DNA片段,其中,多态性DNA带98条,占总扩增片段的63.6%.应用Popgene32软件进行Nei相似性系数和遗传距离计算,并利用UPGMA法构建聚类树状图,把供试的38个蜡梅品种分为7个大类,聚类结果与形态分类结果基本一致.结果表明,RAPD标记可用于蜡梅品种鉴定和亲缘关系探讨.     

运用RAPD技术检测Cu2+对草鱼基因组DNA的影响

对草鱼腹腔注射了CuSO4,抽取注射前后其血液以提取基因组DNA。选用10个随机引物对草鱼基因组DNA进行了RAPD扩增,结果表明:5个引物能产生1～5条扩增带,扩增产物分子大小在200～3500bp之间,其中引物S15和S16能检出用CuSO4染毒前后草鱼基因组DNA的差异。CuSO4可使草鱼基因组DNA发生改变,应对其使用加以限制。     

ISSR 和 RAPD PCR技术对东北地区主推玉米品种的鉴别

运用现代分子生物学简单重复区间序列扩增多态性（ISSR）和随机扩增片段多态性DNA（RAPD）两种分子标记技术， 对吉林省主推6个玉米杂交种及其父本、 母本共计18份基因图谱进行鉴别. 以ISSR为主要检测方法、 RAPD为验证方法，分别从30条ISSR引物中选出4条、 从30条RAPD引物中筛选出2条扩增效果明显、 特异性高的引物. 结果表明， 运用ISSR和RAPD-PCR技术提高了玉米品种鉴定和纯度鉴定的准确性.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.