| ISSR 和 RAPD PCR技术对东北地区主推玉米品种的鉴别 |
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| 引用本文： | 李尚禹,王春艳,张朝燕,李校堃.ISSR 和 RAPD PCR技术对东北地区主推玉米品种的鉴别[J].吉林大学学报(理学版),2006,44(3):509-512. |
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| 作者姓名： | 李尚禹 王春艳 张朝燕 李校堃 |
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| 作者单位： | 1. 长春市产品质量监督检验院， 长春 130012；2. 吉林大学 公共卫生学院, 长春 130021；3. 长春师范学院 生物系， 长春 130032；4. 吉林农业大学 生物工程技术研究院， 长春 130118; 5. 温州医学院 生物与天然药物研究院， 浙江省 温州 325025 |
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| 摘 要： | 运用现代分子生物学简单重复区间序列扩增多态性（ISSR）和随机扩增片段多态性DNA（RAPD）两种分子标记技术， 对吉林省主推6个玉米杂交种及其父本、 母本共计18份基因图谱进行鉴别. 以ISSR为主要检测方法、 RAPD为验证方法，分别从30条ISSR引物中选出4条、 从30条RAPD引物中筛选出2条扩增效果明显、 特异性高的引物. 结果表明， 运用ISSR和RAPD-PCR技术提高了玉米品种鉴定和纯度鉴定的准确性.
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| 关 键 词： | 玉米 ISSR RAPD 品种鉴别 |
| 文章编号： | 1671-5489(2006)03-0509-04 |
| 收稿时间： | 2005-08-09 |
| 修稿时间： | 2005年8月9日 |
Indentification of Varieties among Eitheen Northeast Dominat Maize by ISSR and RAPD-PCR Methods |
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| LI Shang-yu,WANG Chun-yan,ZHANG Chao-yan,LI Xiao-kun.Indentification of Varieties among Eitheen Northeast Dominat Maize by ISSR and RAPD-PCR Methods[J].Journal of Jilin University: Sci Ed,2006,44(3):509-512. |
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| Authors: | LI Shang-yu WANG Chun-yan ZHANG Chao-yan LI Xiao-kun |
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| Institution: | 1. Changchun Department for Product Quality Supervision and Testing, Changchun 130012, China; 2. School of Public Health, Jilin University, Changchun 130021, China; 3. Department of Biology, Changchun Teacher College, Changchun 130032, China; 4. College of Bioengineeringand Technology, Jilin Agricultural University, Changchun 130118, China; 5. School of Biology and Phamaceutical, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China |
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| Abstract: | The gene maps of eighteen maize samples were analyzed by inter-simple sequence repeat ( ISSR) and random amplified polymorphic DNA (RAPD) marker methods. The ISSR method was the primary method and was validated by the RAPD method. 4 highly polymorphic primers were screened out from 30 random ISSR primers and 2 highly polymorphic primers were screened out from 30 random\} RAPD primers. In conclusion, uniting ISSR combined with RAPD can raise the veracity of indentification. |
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| Keywords: | ISSR RAPD |
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