| 蜡梅品种的RAPD分析 |
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| 引用本文： | 芦建国,杜灵娟.蜡梅品种的RAPD分析[J].南京林业大学学报(自然科学版),2007,31(5):109-112. |
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| 作者姓名： | 芦建国 杜灵娟 |
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| 作者单位： | 南京林业大学风景园林学院,江苏,南京,210037 |
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| 基金项目： | 南京林业大学人才培养基金 |
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| 摘 要： | 从200个随机引物中筛选出16个多态性稳定的引物,对38个蜡梅品种进行遗传多样性和亲缘关系RAPD分析.共扩增出154条DNA片段,其中,多态性DNA带98条,占总扩增片段的63.6%.应用Popgene32软件进行Nei相似性系数和遗传距离计算,并利用UPGMA法构建聚类树状图,把供试的38个蜡梅品种分为7个大类,聚类结果与形态分类结果基本一致.结果表明,RAPD标记可用于蜡梅品种鉴定和亲缘关系探讨.
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| 关 键 词： | 蜡梅 品种 遗传多样性 RAPD分析 梅品种 RAPD 分析 Cultivars 品种鉴定 蜡梅 分类结果 形态 树状图 聚类 UPGMA 利用 距离计算 遗传多样性 相似性系数 软件 应用 扩增片段 亲缘关系 稳定 |
| 文章编号： | 1000-2006（2007）05-0109-04 |
| 修稿时间： | 2006-04-28 |
RAPD Analyses of the Chimonanthus praecox Cultivars |
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| LU Jian-guo,DU Ling-juan.RAPD Analyses of the Chimonanthus praecox Cultivars[J].Journal of Nanjing Forestry University(Natural Sciences ),2007,31(5):109-112. |
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| Authors: | LU Jian-guo DU Ling-juan |
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| Institution: | College of Landscape Architecture Nanjing Forestry University, Nanjing 210037,China |
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| Abstract: | Based on 16 primers which are of stable polymorphism selected from 200 random primers,RAPD polymorphism of 38 Chimonanthus praecox cultivars was analyzed.Among a total of 154 fragments observed,98 fragments were polymorphic(63.6%).Popgene 32 software was used to calculate the Nei's genetic distance and a dendrogram was constructed based on UPGMA cluster analysis.These 38 surveyed cultivars were classified into 7 major groups.The results of cluster analysis were consistent with morphological classification.The RAPD molecular marker can be used identify Chimonanthus praecox cultivars and study their relationships of these cultivars. |
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| Keywords: | Chimonanthus praecox Cultivar Genetic diversity RAPD |
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