PHA-L4的纯化及结构分析

通过阴离子交换层析对植物血球凝集素(PHA)提取物进行组分分离、纯化和活性检测,制得了高丝裂原活性的PHA-L4糖蛋白,并利用SDS-PAGE、质谱、N-端氨基酸序列、肽质量指纹谱等分析方法对其结构进行了鉴定.结果表明,PHA-L4是由4个PHA-L蛋白亚基组成的四聚体糖蛋白,相对分子质量为120000,每个亚基N-端第12位天冬酰胺残基被糖基化,糖基化呈现微不均一性.     

β-伴大豆球蛋白中α、α′亚基对大豆分离蛋白薄膜微观结构和功能特性的影响

为了研究β-伴大豆球蛋白中不同亚基组成对大豆分离蛋白薄膜微观结构和功能特性的影响,采用东农47(Wild对照)、α亚基缺失型(α-lack)、α′亚基缺失型(α′-lack)以及α、α′双亚基缺失型[(α+α′)-lack]的大豆为原料提取大豆分离蛋白。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析4种大豆分离蛋白的亚基组成,采用溶液流延法制备不同亚基组成的大豆分离蛋白薄膜,探究不同亚基组成对薄膜流变行为、微观结构、机械强度、耐水性、屏障特性、热稳定性及光学性能的影响。结果表明,不同亚基组成对大豆分离蛋白膜的微观结构和功能特性均有不同影响,与α亚基缺失和α、α′双亚基缺失相比,α′亚基缺失对薄膜结构和功能特性影响最为明显。红外光谱分析结果显示,相较于Wild薄膜,α′亚基缺失加强了蛋白质分子间氢键的相互作用,α′-lack薄膜β折叠含量减少到27.84%,α螺旋和无规则卷曲含量增加到16.83%和13.84%。扫描电镜分析结果显示,α-lack和(α+α′)-lack薄膜纹理形貌较散乱疏松,而α′-lack薄膜无明显气泡且具有平整致密的网络结构。流变特性分析结果发现,α亚基缺失会降低成膜强度,而α′亚基缺失会明显提高膜液的G′,使α′-lack具有更好的潜在成膜能力。与其他3种薄膜相比,α′-lack薄膜具有最大的抗拉伸强度2.31 MPa,最小的水分含量(9.54%)和水溶性(30.81%),最低的水蒸气透过系数[21.89 g·mm/(d·m²·kPa)],表现出优异的热稳定性和可见光阻隔特性。α′亚基的缺失能显著改善大豆分离蛋白薄膜微观结构和功能特性,研究结果旨在为生产高成膜性大豆蛋白系列产品提供理论参考。     

成都麻羊与四川肉用黑山羊地方品种产乳性能研究

测定成都麻羊和四川肉用黑山羊地方品种羔羊哺乳期母羊的产乳量、乳常规营养成分、乳蛋白组分和乳酪蛋白遗传多态性.结果表明,母羊哺乳期的产乳量(kg)成都麻羊、金堂黑山羊、乐至黑山羊、自贡黑山羊和建昌黑山羊相应分别为46.15±1.78,101.67±4.93,85.76±3.36,54.32±6.64和36.65±2.65,成都麻羊极显著低于金堂黑山羊、乐至黑山羊(P<0.01),而极显著高于建昌黑山羊P<0.01)与自贡黑山羊差异不显著(P>0.05);乳中常规营养成分含量(g/L)均较高,范围乳脂为58.59～60.52、乳蛋白为44.18～46.37和乳糖为44.00～47.30;经产母羊常乳主要蛋白组分的相对含量(%)范围α-La为5.35～5.80,β-Lg为14.46～15.18,CN为70.04～71.01,IgG为7.04～7.31,SA为2.02～2.15,品种间差异不显著(P>0.05);酪蛋白电泳染色显示出α-CN和β-CN图带,α-CN均呈现4种不同的带型αS1-CN(H),αS1-CN(L)、αS2-CN(CC)型和αS2CN(CD),其中均以αS2-CN(CD)占优势.β-CN呈现单态,带型为β-CN(AB).     

藻红蛋白基因部分序列的克隆和顺序分析

报道了真核红藻－龙须菜的藻红蛋白亚基基因的部分序列,位于β亚基的近碳端,α亚基的近氮端,包括β亚基部分３０９个核苷酸,α亚基部分１６２个核苷酸,还有间隔部分５５个核苷酸,可编码β近碳端的１０２个氨基酸和α近氮端的５４个氨基酸,并与目前已知的相应序列做了比较,该序列与已知红藻ＰＥ亚基基因相应部分的相似性为７７．９％和８１．１％。对应的氨基酸序列的相似性为７９．５％到８６．５％,蛋白质序列的保守性高于     

HPLC及LC-ESI-MS分离鉴定B-藻红蛋白的亚基(英文)

摘要:为了分析B-藻红蛋白的三种亚基,采用反相HPLC并通过二极管阵列/紫外可见光检测器和荧光检测器,以及LC-电喷雾/MS质谱检测器进行在线检测与鉴定.结果表明,根据在线检测的光谱特征,α,β和3个明显的γ亚基都能成功地被C4柱分离,且α和β亚基均在555 nm处有最大吸收峰,而γ亚基在493nm和555nm处有最大吸收峰.并结合质谱信息,可鉴别出α和β亚基,它们的相对分子质量分别为18977和20330.说明结合上述几种检测技术可为B-藻红蛋白的亚基鉴定提供可靠的结果.     

酸性条件下大豆分离蛋白的亚基解离

研究了不同pH值下大豆分离蛋白的Zeta电位、超速离心电泳、溶解性、荧光光谱及二级结构的变化及其规律,并进一步探讨了酸性条件下大豆分离蛋白亚基解离的机理.结果表明:酸性条件下大豆分离蛋白的亚基解离主要是由分子间静电斥力导致的,而且大豆球蛋白的解离程度远大于β-大豆伴球蛋白;溶液pH值在2.0 ～3.0之间时,Zeta电...     

β-淀粉样蛋白基元序列寡聚化及纤维化的功能研究

【目的】β-淀粉样蛋白形成的寡聚体是引起阿尔茨海默症(Alzheimer disease,AD)发病的主要原因之一,研究β-淀粉样蛋白各段序列在寡聚和纤维化过程中的作用,以便更好地阐明该蛋白的寡聚机制和毒性作用。【方法】以C-端及N-端删节的6种β-淀粉样肽段作为研究对象,通过硫磺素T荧光检测、Tris-Tricine电泳、透射电镜等方法定性定量分析这些肽段的寡聚化和纤维化能力。【结果】1)氨基酸37~42具有增强Aβ寡聚化和纤维化的功能;2)氨基酸18~36对于寡聚和纤维化很重要,但不能缺少N-端的参与;3)Aβ1-17参与β-淀粉样蛋白长纤维的形成。【结论】β-淀粉样蛋白各段氨基酸序列对该蛋白寡聚和纤维化所贡献的不同作用,这对于β-淀粉样蛋白毒性机制的研究起到一定的帮助作用。     

液相色谱-高分辨串联质谱法检测牛奶中A1和A2 β-酪蛋白

建立一种基于液相色谱\|高分辨串联质谱（LC-HRMS/MS）方法检测牛奶中A1和A2 β-酪蛋白. 采用等电点沉淀法从牛奶中提取酪蛋白, 经胰蛋白酶酶解后, 将酶解产物进行LC-HRMS/MS分析, 选择含有差异氨基酸残基（第67位）的肽段作为特征肽段对A1和A2 β-酪蛋白进行区分. 采用Tris-HCl缓冲溶液充分溶解酪蛋白, 在酶解反应体系中添加乙腈以提高酶解效率. 采用高分辨质谱和串联质谱技术, 进一步排除杂质产生的干扰信号. 采用该方法对市售4种普通牛奶和2种A2牛奶进行检测, 结果表明， 普通牛奶中A1 β-酪蛋白的含量约为A2 β-酪蛋白含量的2~4倍, 个别A2牛奶样品含微量A1 β-酪蛋白. 该方法可检测牛奶中的A1和A2 β-酪蛋白, 具有高可靠性、 高灵敏度等优点, 为A2奶源筛选和质量控制提供支持.     

β-淀粉样蛋白基元序列寡聚化及纤维化的功能研究

【目的】β-淀粉样蛋白形成的寡聚体是引起阿尔茨海默症(Alzheimer disease,AD)发病的主要原因之一,研究β-淀粉样蛋白各段序列在寡聚和纤维化过程中的作用,以便更好地阐明该蛋白的寡聚机制和毒性作用。【方法】以C-端及N-端删节的6种β-淀粉样肽段作为研究对象,通过硫磺素T荧光检测、Tris-Tricine电泳、透射电镜等方法定性定量分析这些肽段的寡聚化和纤维化能力。【结果】1)氨基酸37~42具有增强Aβ寡聚化和纤维化的功能;2)氨基酸18~36对于寡聚和纤维化很重要,但不能缺少N-端的参与;3)Aβ1-17参与β-淀粉样蛋白长纤维的形成。【结论】β-淀粉样蛋白各段氨基酸序列对该蛋白寡聚和纤维化所贡献的不同作用,这对于β-淀粉样蛋白毒性机制的研究起到一定的帮助作用。
     

鹿茸水提取物及其蛋白酶解物对小鼠 脾淋巴细胞增殖的影响

研究了鹿茸水提取物中非蛋白成分、粗蛋白酶解物及分离组分对小鼠脾淋巴细胞增殖的影响.非蛋白成分和酶解物的分离分别采用HPLC法和超滤法.应用WST-8法检测各分离组分对小鼠脾淋巴细胞增殖的影响,采用高分辨质谱对非蛋白成分进行初步鉴定.结果显示,非蛋白成分对未经刀豆蛋白A(ConA)诱导的小鼠脾细胞增殖的促进作用优于其分离组分,Fr.Ⅲ和Ⅳ对ConA诱导的脾淋巴细胞增殖有抑制作用,初步鉴定其为碱基和核苷酸的混合物.分子量大于10 kDa的肽段,单独可刺激小鼠脾细胞增殖,且对ConA诱导的T淋巴细胞增殖有抑制作用.肽段分子量大小对其免疫调节活性影响较大,但并未呈现直接的相关性.肽的氨基酸组成、序列及构型等也是影响其活性的重要因素.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

正~~     

An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.