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1.
探索了米邦塔食用仙人掌组培快繁技术。得出不定芽诱导使用培养基MS+6-BA3.0mg/L+NAA0.1mg/L,外植体分化率达77.3%;继代增殖使用培养基MS+6-BA1mg/L+KT0.5mg/L+NAA0.3mg/L,试管苗平均增殖倍数达10.19,结合反复利用原外植体的方法,增殖效率可进一步提高;生根培养基使用1/2 MS+NAA0.1mg/L+IBA0.1mg/L,生根率达100%,且根系发育优良;驯化及试管苗入地后,以保湿为主要管理措施,可保证移栽成活率在95%左右。  相似文献   

2.
对糯米藤(Memorialis hirta BL.Wedd.)进行了离体快繁研究。叶片和茎段在附加NAA0.5mg/L+KT1.0mg/L或NAA0.5mg/L 6-BA 1.0mg/L的MS培养基上,诱导形成愈伤组织后再分化形成植株;腋芽在附加NAA0.1mg/L KT1.0mg/L或NAA0.1mg/L 6-BA1.0mg/L或IAA0.1mg/L 6-BA 1.0mg/L的MS培养基上诱导出丛生芽,并再生成植株。  相似文献   

3.
兰州百合和野百合组织培养及快速繁殖研究   总被引:42,自引:1,他引:42  
以兰州百合(Lilium davidii Var.Unicolor (Hoog)Cotton)和野百合(Lilium brownii F.E.Brown ex Miellez) 鳞茎及叶片为外植体获得再生植株,并建立起快速无性繁殖系。兰州百合鳞茎的最佳诱导培养基为MS+0.6-1.0mg/LBA+0.2mg/LNAA;芽增殖培养基选MS+0.5-0.6mg/LBA 0.1mg/L NAA;生根培养基为1/2MS+0.2mg/l IAA 0.1%活性炭。野百合鳞茎及叶的最佳诱导培养基分别为MS+0.1-0.5mg/LBA 0.1-0.2mg/L NAA或0.5mg/L IAA和MS+0.7mg/L BA 0.07mg/L NAA;鳞茎诱导的芽的增殖培养基为MS+0.2-0.4mg/L BA 0.1mg/L NAA;生根培养基为1/2MS+0.15mg/L NAA 0.1%活性炭。  相似文献   

4.
选用741杨的茎段和生根组培苗叶片为外植体,采用不同浓度组合的BA和NAA对741杨的离体快繁和叶片再生体系进行了研究。结果表明:741杨芽增值的最佳培养基为MS+6-BA 0.5mg/L+NAA 0.1mg/L;最佳生根培养基为MS+IBA 0.3mg/L;741杨叶片不定芽诱导的最佳培养基为MS+6-BA 1.0mg/L+NAA 0.1mg/L。试验还研究了不同着生位置的叶片以及叶片在培养基上的放置方式对741杨叶片再生的影响。  相似文献   

5.
蒜头果组织培养再生系统的初步研究   总被引:7,自引:0,他引:7  
以蒜头果(Malania oleifera)种子萌发获得的无菌苗进行离体培养的初步研究.结果表明:MS+6-BA1.5mg/L+NAA0.1mg/L激素组合能够较好地诱导芽的初始分化和增殖;适宜的继代增殖培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;采用1/2MS+IBA0.5mg/L为生根培养基,生根率为45.0%;移栽到河沙的生根苗成活率为52.5%.  相似文献   

6.
将Alyssum maritimum(L.)Lain.的种子灭菌后,分别接人培养基MS+6-BA1.0mg/L+NAA0.2mg/L、MS+6-BA1.5mg/L+NAA0.2mg/L、MS+6-BA2.0mg/L+NAA0.2mg/L、MS+6-BA2.5mg/L+NAA0.2mg/L中,得到无菌苗.取20d的无菌苗叶子切成3minx2mm的小块,接人同样的培养基中诱导芽,在MS+6-BA2.5mg/L+NAA0.2mg/L培养基上分化得到芽,将芽转入不同NAA浓度MS培养基上生根,实验表明,A.maritimum(L.)Lam.芽诱导最适合的培养基是MS+6-BA2,5mg/L+NAA0.2mg/L.  相似文献   

7.
以玉簪新品种的幼芽为外植体进行无性快繁试验,结果表明:适宜的启动培养基为MS+6-BA3.0+2,4-D1.0(mg/L),该培养基有很好的诱导外植体产生不定芽苗的效果;适宜的增殖培养基为:MS+6-BA1.0+NAA0.1(mg/L),形成的丛生苗比较健壮;生根时MS培养基较为合适。  相似文献   

8.
报道了双青35号菜豆不同外植体在含有不同激素的培养基上诱导、分化及生根情况,并建立其高效再生体系.研究结果表明:愈伤组织诱导和增殖培养基为MS+6-BA2.0mg/L+NAA2.0mg/L,不定芽再生培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,生根培养基为1/2MS+IAB3.0mg/L+0.1%活性炭是较佳的.  相似文献   

9.
以八宝景天茎段为外植体,以MS为基本培养基,对八宝景天外植体消毒、不定芽诱导、继代增殖和生根培养基进行筛选试验,建立了八宝景天组织培养快繁技术体系。结果表明:75%C_2H_5OH 30s+0.1%HgCl_2 6min为八宝景天茎段消毒的最佳方法;MS+6-BA 2.0mg/L+NAA 0.1 mg/L为不定芽诱导的最佳培养基;MS+6-BA 3.0mg/L+NAA 0.1mg/L为继代增殖培养的最佳培养基;不定芽诱导生根的最佳培养基为1/2MS+NAA 0.1 mg/L或1/2MS+IBA 0.1 mg/L,生根率达100%。  相似文献   

10.
菊花的组织培养研究   总被引:4,自引:1,他引:4  
以菊花侧芽茎尖做外植体,通过二次表面灭菌,得到茎尖无菌培养物.根据长出丛生芽的芽数的多少,筛选出适合菊花茎尖离体培养的培养基.MS+6-BA5.0mg/L+NAA0.01mg/L的固体培养基适宜于诱导侧芽生长出丛生芽;丛生芽转入到MS+6-BA3.0mg/L+NAA0.01mg/L培养基上进行继代培养扩大增殖:取长2~3cm的芽苗在1/2MS+NAA0.1mg/L培养基上生根并形成完整植株.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

13.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

14.
AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。  相似文献   

18.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

19.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

20.
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