| 米邦塔食用仙人掌组培快繁技术研究 |
| |
| 引用本文： | 陈芳,李桂芳,魏鹏.米邦塔食用仙人掌组培快繁技术研究[J].石河子大学学报,2006,24(5):596-598. |
| |
| 作者姓名： | 陈芳 李桂芳 魏鹏 |
| |
| 作者单位： | 石河子大学生命科学学院,新疆石河子832003 |
| |
| 摘 要： | 探索了米邦塔食用仙人掌组培快繁技术。得出不定芽诱导使用培养基MS＋6-BA3．0mg／L＋NAA0．1mg/L，外植体分化率达77．3％；继代增殖使用培养基MS＋6-BA1mg／L＋KT0．5mg／L＋NAA0．3mg/L，试管苗平均增殖倍数达10.19，结合反复利用原外植体的方法，增殖效率可进一步提高；生根培养基使用1／2 MS＋NAA0．1mg／L＋IBA0．1mg／L，生根率达100％，且根系发育优良；驯化及试管苗入地后，以保湿为主要管理措施，可保证移栽成活率在95％左右。
|
| 关 键 词： | 食用仙人掌 组培快繁 增殖效率 |
| 文章编号： | 1007-7383(2006)05-0596-03 |
| 收稿时间： | 2006-05-07 |
| 修稿时间： | 2006年5月7日 |
A Study on the Rapid Propagation Method of Opuntia vilpa alta Haw.Plant Tissue Culture in Vitro Plant Tissuecultuk |
| |
| CHEN Fang,LI Gui-fang,WEI Peng.A Study on the Rapid Propagation Method of Opuntia vilpa alta Haw.Plant Tissue Culture in Vitro Plant Tissuecultuk[J].Journal of Shihezi University(Natural Science),2006,24(5):596-598. |
| |
| Authors: | CHEN Fang LI Gui-fang WEI Peng |
| |
| Institution: | College of Life Sciences, Shihezi University, Shihezi, Xinjiang 832003, China |
| |
| Abstract: | The results showed explants of differentiation rate attained to 77.3% with medium MS+6-BA 3.0mg/L+NAA 0.1mg/L in bud induction;the tube seedling multiplication attained 10.19 in subculture with medium MS+6-BA 1mg/L+KT 0.5mg/L+NAA 0.3mg/L.The multiplication efficiency migh t further enhance if explants were used repeatedly.Rooting rate attained to 100% with medium 1/2 MS+NAA 0.1mg/L+IBA 0.1mg/L,and growth of the rooting seedling was fine;After sunlight domesticating,the seedling transplanting survival rate attained to about 95%. |
| |
| Keywords: | Opuntia vilpa alta Haw mieropropagation muhiplieation efficiency |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
