Laminin五肽对胃癌细胞系MGc—803的体外抑制作用

描述了Ｌａｍｉｎｉｎ五肽（ＹＩＧＳＲ）对胃癌细胞系ＭＧｃ－８０３的体外抑制作用，在多肽浓度为１ｍｇ／ｍｌ时抑制率高达７３％。抑制作用的机理可能是Ｌａｍｉｎｉｎ五肽竞争了肿瘤细胞上Ｌａｍｉｎｉｎ受体，从而封闭了肿瘤细胞对基底膜的结合及增殖。因此，利用Ｌａｍｉｎｉｎ五肽一类的药物有可能为肿瘤术后防止扩散提供一个新方法。     

拓扑特肯体内外抗肿瘤作用的研究

测定了拓扑特肯对５种体外培养人肿瘤细胞株的生长抑制作用及对小鼠移植性肿瘤的抑制作用。结果显示拓扑特肯对各体外肿瘤细胞都有明显的细胞毒作用,除了人胃癌ＢＧＣ－８０３的ＩＣ５０为３．８７μｇ／ｍＬ外,对其它瘤株的ＩＣ５０均小于０．１μｇ／ｍＬ。在１．０ｍｇ／ｋｇ,２．０ｍｇ／ｋｇ剂量下,拓扑特肯对小鼠肉瘤Ｓ１８０的抑制率分别为３２．０％、３３．８％;对小鼠肝癌Ｈ２２的抑制率分别为３１．４％、４２．１％     

水稻根谷氨酰胺合成酶同工酶某些性质研究

对水稻根两种不同形式的胺合成酶进行了动力学性质研究，ＧＳｍ对热比较稳定，而ＧＳｂ比较敏感，ＧＳ２ａ的最适温度为５５℃，ＧＳｒｂ为４３℃，ＧＳｒｃ的最适ＰＨ都为７．５ＧＳｒａ对Ｌ－谷氨酸，经胺和ＡＴＰ的表观Ｋｉａ分别为４．１６，０．６０和０．３４，而ＧＳ分别为５１．７３，０．６０和０．５８，ＭＳＯ对两均表现为竞争性抑制作用。     

谷氨酸阴离子和晶种对晶体成核与生长的影响

研究了谷氨酸阴离子（Ｇ－）和晶种对其晶体二次成核与生长的影响．结果表明成核速率随晶种粒度和晶种量减少而减少；在高过饱和度时Ｇ－对成核有促进作用，而在低过饱和度时则有抑制作用；晶体生长速率随粒度减小而增大；Ｇ－对晶体生长有抑制作用；结晶时使用粒度小的晶种可得到粒度分布较均匀的晶体．     

固相合成胸腺五肽的分离与纯化

胸腺五肽(TP5)是一种很有发展前景的药物，具有胸腺素的全部生理功能固相合成法是胸腺五肽生产的主流技术，目前，对固相合成的五肽一般采用反相色谱技术纯化，但效率低、成本高，仅限于小规模制备实验采用离子交换法纯化，并进行了方案优化，发现在pH4．4醋酸盐缓冲液平衡、盐梯度洗脱条件下纯化TP5，一步分离纯度可达98．23％该法可以满足大规模制备的要求，且成本很低     

维生素A诱导Shope肿瘤细胞凋亡

目的：探讨维生素A抑制Shope肿瘤细胞生长的机理。方法：电镜观察Shope肿瘤细胞凋亡的形态学改变：琼脂糖凝胶电泳进行DNA片段化；流式细胞仪检测凋亡细胞的百分比。结果：电镜下可VA诱导的Shope肿瘤细胞变小，细胞浆固缩，染色质聚集并靠近核膜，在1．5％琼脂糖凝胶电泳中，Shope肿瘤细胞DNA呈现明显的阶梯状图谱。流式细胞仪检测经10－5MV作用24h、48h、72h后，凋亡细胞百分比分别为：17．1％、22．4％、32．4％。结论：以上结果表明，VA对Shope肿瘤细胞有明显的抑制作用。     

抑癌基因p27在肿瘤细胞MCF7中的表达

抑癌基因p27是一个细胞周期依赖性激酶抑制子CKI(CDK inhibitor),对细胞周期起着负调控的作用,将p27基因克隆到表达载体pcDNA,转染到肿瘤细胞MCF7中,筛选到稳定表达株．p27基因的过量表达确实对肿瘤细胞的生长产生了抑制作用,并且引发了部分肿瘤细胞的凋亡．     

青霉素G钠对阴生植物绿巨人愈伤组织诱导分化及试管苗 …

报道了青霉素Ｇ钠对绿巨人愈伤组织诱导、分化及苗生长的初步结果。试验表明：青霉素Ｇ钠对诱导叶片愈伤组织的产生有抑制作用，而对愈伤组织的分化有促进作用，效果最好的浓度为６０万ｕ·Ｌ＾－１，平均每块愈伤组织可分化７．０个不定芽；低浓度的青霉素Ｇ钠（２０万ｕ·Ｌ＾－１）对试管苗的生长有促进作用，同时也促进根系的发育；而高浓度的青霉素Ｇ钠对试管苗的生长有抑制作用，并随着浓度的提高而增强，在生根培养基中添加低     

气味沙雷氏菌蛋白组分分离及其体外抗癌活性

用ＳＤＳ－ＰＡＧＥ对经超声波处理的气味沙雷氏菌全菌体进行分离，切胶分别洗脱，经丙酮沉淀、脱盐处理后得到九个蛋白组分，将九个组分分别用Ｌ９２９，ＱＧＹ肿瘤细胞为靶细胞进行细胞毒性试验（ＭＴＴ），经初步鉴定从此且分具有抗癌细胞活性，组分Ｆ６相对分子质量范围为２８０００－３３０００，其抑制５０％细胞生长的蛋白浓度（ＩＣ５０）；对Ｌ９２９细胞为０．０４６ｍｇ／ｍｌ对ＱＧＹ细胞为０．０１２５０ｍｇ／ｍｌ，活     

海藻真菌抗肿瘤活性的初步研究

从福建漳州浯屿岛和漳州浮宫海岸潮问带采集到的10种海藻中分离到66株海藻真菌．采用MTT法对其发酵液乙酸乙酯抽提物的抗肿瘤活性进行检测．发现34株菌株对KB和／或Raji肿瘤细胞具有显著的抑制活性,占供测菌株的52％．其中对KB细胞有抑制作用的菌株有21株．对Raji细胞有抑制作用的菌株有30株．对两种肿瘤细胞均有抑制作用的菌株有17株．分别占活性菌株的62％、88％和50％．实验结果表明,海藻真菌是潜在的抗肿瘤药物资源．     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.