HMBA和亚硒酸钠对人胃腺癌细胞的诱导分化

用５×１０（－３）ｍｏｌ／Ｌ环六亚甲基双乙酰胺和３×１０（－６）ｍｏｌ／Ｌ亚硒酸钠诱导剂组合处理人胃腺癌ＭＧｃ８０－３细胞，结果细胞的群体增殖和ＤＮＡ合成均受到显著抑制，生长抑制率最高达７２．８％，ＤＮＡ合成的抑制率达８４．１％，液门测定的ＣＰＭ值也较诱导处理之前降低了５．２６倍；同时，ＭＧＣ８０－３细胞的表型由典型恶性特征向相应正常细胞类似的形态结构特征转变，细胞对裸鼠的致瘤性也明显降低（Ｐ值＜０．０５）·表明ＨＭＢＡ和亚硒酸钠的组合能够有效地诱导人胃腺癌细胞的分化，而且这种诱导分化作用比单独使用其中一种诱导物所产生的效果更显著．     

西瓜种植物RAPD分析影响因子的研究

以西瓜种内的１２个品种（系）为模板ＤＮＡ,研究了影响ＲＡＰＤ扩增效果的因素。结果表明,模板ＤＮＡ、Ｔａｑ酶、随机引物、Ｍｇ２＋浓度以及ＰＣＲ反应程序在ＲＡＰＤ分析中都具有重要的作用。根据以上因子的研究结果,建立了适合西瓜种植物的ＲＡＰＤ分析的技术体系：１０μＬ反应液中,模板１０ｎｇ／１０μＬ,引物０５μｍｏｌ／Ｌ,ＴｒｉｓＨＣｌ（ｐＨ８３）５０ｍｍｏｌ／Ｌ,ＢＳＡ５００μｇ／ｍＬ,ＭｇＣｌ２２５ｍｍｏｌ／Ｌ,ＴａｑＤＮＡ０６Ｕ／１０μＬ,ｄＮＴＰｓ２００μｍｏｌ／Ｌ,１０％蔗糖４００和１ｍｍｏｌ／Ｌ甲酚红。     

视黄酸对BEL—7402细胞酶活性及基因表达的影响

人肝癌ＢＥＬ－７４０２细胞分别以５μｍｏｌ／Ｌ、２０μｍｏｌ／Ｌ和５０μｍｏｌ／Ｌ的视黄酸（ＲＡ）处理后,细胞生长明显受抑,平均细胞群体倍增时间延长,并呈ＲＡ浓度及处理时间依赖性关系．经２０μｍｏｌ／ＬＲＡ处理的细胞中,ｒ－谷氨酰转肽酶（ｒ－ＧＴ）活性自给药后第２天即持续下降,至第６天降至４４．５％,而酪氨酸－α－酮戊二酸转氨酶（ＴＡＴ）活性则持续增高,至第６天较对照组高近１倍．经２０μｍｏｌ／ＬＲＡ处理５ｄ后,ＲＮＡ斑点杂交分析表明ｐ５３基因转录加强,而Ｎ－ｒａｓ基因表达下降,提示该两基因的表达变化可能在ＢＥＬ－７４０２细胞的生长抑制及逆转过程中起重要作用,有关机制尚待深入研究．     

天然抗氧化剂Isoverbascoside对人胃腺癌细胞生长和超微…

ＭＧｃ８０－３细胞经２０μｍｏｌ／Ｌ抗氧化剂Ｉｓｏｖｅｒｂａｓｃｏｓｉｄｅ处理后，细胞生长曲线与分裂指数显下降，倍增时间延长，生长抑制率达５７．８％，电镜观察结果表明，Ｉｓｏｖｅｒｂａｓｃｏｓｉｄｅ处理的细胞，核质比例下降，核形规则，核仁体积缩小，数量减少，核内异染色质减少，线粒体形态结构典型，细胞表面微绒毛减少等显变化，说明天然抗氧化剂Ｉｓｏｖｅｒｂａｓｃｏｓｉｄｅ改变了ＭＧｃ８０－３细胞的     

天然抗氧化剂Isoverbascoside对人胃腺癌细胞生长和超微结构的影响

ＭＧｃ８０－３细胞经２０μｍｏｌ／Ｌ抗氧化剂ｌｓｏｖｅｒｂａｓｃｏｓｉｄｅ处理后，细胞生长曲线与分裂指数显著下降，倍增时间延长，生长抑制率达５７．８％．电镜观察结果表明：ｌｓｏｖｅｒｂａｓｃｏｓｉｄｅ处理的细胞，核质比例下降，核形规则，核仁体积缩小，数量减少，核内异染色质减少；线粒体形态结构典型；细胞表面微绒毛减少等显著变化．说明天然抗氧化剂Ｉｓｏｖｅｒｂａｓｃｏｓｉｄｅ改变了ＭＧｃ８Ｏ－３细胞的恶性表型特征，具有明显的诱导分化作用．     

川芎嗪对刺激─分泌偶联模型功能活动的抑制作用

用胶原酶消化分离小牛肾上腺髓质嗜铬细胞,１０－４ｍｏｌ／Ｌ乙酰胆碱（Ａｃｈ）促进细胞儿茶酚胺的分泌,其作用具有Ｃａ２＋依赖性,回归方程ｒ＝－３．２９０＋５．２７５ｘ,相关系数Ｒ＝０．９９１０＞＞α０．０１＝０．７９８０．高Ｋ＋（６５ｍｍｏｌ／Ｌ）使膜去极化,也促进细胞儿茶酚胺的分泌,在一定范围内与Ｃａ２＋浓度呈正相关,回归方程ｙ＝１．３７６＋５．３３７ｘ,相关系数Ｒ＝０．９８５６＞＞α０．０１＝０．７９８１．磷酸川芎嗪（ＴＭＰ）浓度为１０－４～１０－７ｍｏｌ／Ｌ时,能抑制Ａｃｈ促进的分泌作用,１０－３～１０－７ｍｏｌ／Ｌ时能抑制高Ｋ＋促进的分泌作用．     

栉孔扇贝四倍体的研究：I：细胞松驰素B诱导

利用细胞松驰素Ｂ抑制栉孔扇贝Ｃｈｌｌａｍｙｓ（Ａｚｕｍａｐｅｃｔｅｎ）ｆａｒｒｅｒｉ受精卵的第一极体的放出和第一次有丝分裂获得四倍体，在２０℃条件下，卵子受精后１０ｍｉｎ，０．５μｇ／ｍｌ的ＣＢ持续处理１０ｍｉｎ，２０ｍｉｎ抑制第一极体排出，结果三倍体率和四倍体率分别为３８．３８％，３９．７５％和２８．４２％，３０．２４％，在２０℃条件下，卵子受精后１ｈ，０．５μｇ／ｍｌ的ＣＢ持续处理１０，１５，     

DApBM—V（Ⅴ）—Mn（Ⅱ）—Twenn—80体系光度法测定钒（Ⅴ）

在磷酸介质中，在Ｍｎ和Ｔｗｅｅｎ－８０存在下，ＤＡｐＢＭ与钒生成橙黄色化合物，产物至少可稳定３．５ｈ，最大吸收波长为４８５ｎｍ。摩尔吸光系数ε＝４．０１×１０＾５Ｌ．ｍｏｌ＾－１．ｃｍ＾－１和ε＝１．５７×１０＾４Ｌ．ｍｏｌ＾－１．ｃｍ＾－１，钒量在０．５－３．０μｇ／２５ｍｌ及３．０－１０．０μ／２５ｍｌ间符合比尔定律。     

小麦根尖细胞有丝分裂同步化诱导与中期染色体分离

本研究以小麦根尖分生组织为材料，用Ｈｕ和ＡＰＭ双阻断法对细胞有丝分裂中期同步化和前期、后期、末期和部分同步化诱导作了探讨，并对中期染色体分离也作了研究，所得结构表明，用１．２５ｍ ｍｏｌ／Ｌ Ｈｕ、４μｍ ｍｏｌ／Ｌ ＡＰＭ处理，可使细胞有丝分裂中期指数（Ｍｅｔ．Ｉ）提高至５０％，经１８ｈ单独处理，去除Ｈｕ作用，水培使期恢复生长至８ｈ时，可检测到１１．５％的前期分裂细胞。Ｈｕ单独处理也可诱导后期和     

去甲斑蝥素对人乳腺癌细胞MCF-7的杀伤效应及其可能机制

用１０μｇ／ｍＬ去甲斑蝥素（ＮＣＴＤ）作用于人乳腺癌细胞,以ＭＴＴ法、流式细胞仪、台盼蓝排斥法和透射电镜等方法分别观察ＮＣＴＤ对癌细胞的增殖抑制作用及对细胞周期和细胞凋亡的影响。结果表明：ＮＣＴＤ对乳腺癌细胞的杀伤作用有时间和剂量依赖性,药物作用２４ｈ后,Ｇ１期细胞减少,Ｇ２＋Ｍ期细胞明显增多,凋亡细胞百分比升高并可见到凋亡细胞的形态学变化。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to