| 西瓜种植物RAPD分析影响因子的研究 |
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| 引用本文： | 赵虎基,李红霞.西瓜种植物RAPD分析影响因子的研究[J].石河子大学学报,1999,3(2):87-92. |
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| 作者姓名： | 赵虎基 李红霞 |
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| 作者单位： | 石河子大学农学院园艺园林工程系，石河子大学分析测试中心 |
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| 摘 要： | 以西瓜种内的１２个品种（系）为模板ＤＮＡ，研究了影响ＲＡＰＤ扩增效果的因素。结果表明，模板ＤＮＡ、Ｔａｑ酶、随机引物、Ｍｇ２＋浓度以及ＰＣＲ反应程序在ＲＡＰＤ分析中都具有重要的作用。根据以上因子的研究结果，建立了适合西瓜种植物的ＲＡＰＤ分析的技术体系：１０μＬ反应液中，模板１０ｎｇ／１０μＬ，引物０５μｍｏｌ／Ｌ，ＴｒｉｓＨＣｌ（ｐＨ８３）５０ｍｍｏｌ／Ｌ，ＢＳＡ５００μｇ／ｍＬ，ＭｇＣｌ２２５ｍｍｏｌ／Ｌ，ＴａｑＤＮＡ０６Ｕ／１０μＬ，ｄＮＴＰｓ２００μｍｏｌ／Ｌ，１０％蔗糖４００和１ｍｍｏｌ／Ｌ甲酚红。
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| 关 键 词： | 西瓜 RAPD 影响因子 |
Studies on Affecting Factors in the Analysisof the Plant of Clanatus |
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| Zhao HujiLe JinhuaLi HongxiaCui Huimei.Studies on Affecting Factors in the Analysisof the Plant of Clanatus[J].Journal of Shihezi University(Natural Science),1999,3(2):87-92. |
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| Authors: | Zhao HujiLe JinhuaLi HongxiaCui Huimei |
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| Institution: | Zhao Huji1Le Jinhua1Li Hongxia2Cui Huimei1 |
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| Abstract: | The studies of affectors affecting RAPD were carried out by the 12 species of CLanatus as template DNAThe results showed that the purification of template DNA,the concentrations of template DNA,Taq polymerase,random primer,Mg2 and the PCR programe played an important role in RAPD analysisBased on the researches above,a technical system for RAPD analysis in species of Clanatus was established:in 10 L reaction solution,containing 10 ng/10 L template DNA,05 mol/L random primer,50 mmol/L TrisHCl(pH83),500 g/mL BSA,25 mmol/L MgCl2,06 U/10 L Taq DNA,200 mol/L dNTPs |
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| Keywords: | Clanatus RAPD affecting factors |
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