| 弧菌广谱性外膜蛋白抗原筛选及其单抗的交叉免疫原性研究 |
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| 引用本文： | 林海英,马振宁,郑允权,郭养浩,唐凤翔.弧菌广谱性外膜蛋白抗原筛选及其单抗的交叉免疫原性研究[J].福州大学学报(自然科学版),2012,40(4):535-540. |
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| 作者姓名： | 林海英 马振宁 郑允权 郭养浩 唐凤翔 |
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| 作者单位： | 福州大学药物生物技术研究所;福建省医疗器械与医药技术重点实验室 |
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| 基金项目： | 福建省科技厅重点资助项目(2009N40101,2010N0014);福州大学校基金资助项目(04660022352) |
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| 摘 要： | 采用生物信息学方法分析几种病原性弧菌OmpK、OmpU和OmpW蛋白种间和种内的同源性,筛选高保守性蛋白OmpW.成功构建具备稳定分泌抗体IgG3的细胞株S5C10,鉴定其对5种弧菌具有特异性交叉免疫反应,而对9种非弧菌无免疫反应.研究结果显示OmpW可作为广谱性疫苗成分,其单抗可特异性检测多元弧菌.
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| 关 键 词： | 弧菌 广谱性 外膜蛋白 单抗 交叉免疫原性 |
Screening and cross-immunogenicity of broad-spectrum outer membrane protein from Vibrio |
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| LIN Hai-ying,MA Zhen-ning,ZHENG Yun-quan,GUO Yang-hao,TANG Feng-xiang.Screening and cross-immunogenicity of broad-spectrum outer membrane protein from Vibrio[J].Journal of Fuzhou University(Natural Science Edition),2012,40(4):535-540. |
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| Authors: | LIN Hai-ying MA Zhen-ning ZHENG Yun-quan GUO Yang-hao TANG Feng-xiang |
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| Institution: | 1.Institute of Pharmaceutical Biotechnology and Bioengineering,Fuzhou University,Fuzhou,Fujian 350002,China; 2.Fujian Key Lab of Medical Instrumentation and Pharmaceutical Technology,Fuzhou,Fujian 350002,China) |
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| Abstract: | Homology of OmpK,OmpU and OmpW proteins from the same and different pathogenic Vibrio species was bioinformaticlly analysed,based on that highly conserved OmpW protein was selected.Hybridoma S5C10 was successfully constructed and stablly secreted IgG3.mAb secreted from S5C10 could specifically cross-reacted with five Vidrio species,but none with nine non-Vibrios.The results showed that OmpW protein could be abroad-spectrum antigen,use of that mAb could specifically detected multi-Vibrios. |
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| Keywords: | Vibrio broad-spectrum outer membrane protein McAb ross-immunogenicity |
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