| 栽培和野生大豆核酮糖-1,5-二磷酸羧化酶的结晶及其大小亚基等电聚焦分析 |
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| 引用本文： | 袁庆,杨茹,曹凯鸣,朱俭,顾其敏.栽培和野生大豆核酮糖-1,5-二磷酸羧化酶的结晶及其大小亚基等电聚焦分析[J].复旦学报(自然科学版),1999(5). |
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| 作者姓名： | 袁庆 杨茹 曹凯鸣 朱俭 顾其敏 |
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| 摘 要： | 栽培大豆和野生大豆叶片的蛋白抽提液经硫酸铵盐析、Ｓｅｐｈａｄｅｘ Ｇ－５０柱层析以及浓缩透析等步骤，分别得到核酮糖－１，５－二磷酸羟化酶（简称Ｒｕｂｉｓｃｏ）的结晶．经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ酶标抗体技术鉴定证实为Ｒｕｂｉｓｃｏ．用ＩＥＦ－ＰＡＧＥ方法分析这２种不同进化类型大豆的Ｒｕｂｉｓｃｏ 大小亚基，发现其具有高度同源性
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| 关 键 词： | 大豆 核酮糖-1 5-二磷酸羧化酶 结晶 等电聚焦 |
Crystallization of Rubisco and Its Isoelectrofocusing Analysis of Large and Small Subunits from Wild and Cultivated Soybean |
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| YUAN Qing,YANG Ru,CAO Kai ming,ZHU Jian,GU Qi min.Crystallization of Rubisco and Its Isoelectrofocusing Analysis of Large and Small Subunits from Wild and Cultivated Soybean[J].Journal of Fudan University(Natural Science),1999(5). |
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| Authors: | YUAN Qing YANG Ru CAO Kai ming ZHU Jian GU Qi min |
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| Institution: | Department of Biochemistry |
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| Abstract: | Rubisco (Ribulose 1,5 bisphosphate Caboxylase) was purified and crystallized from Glycine max and Glycine soja leaves. The results of SDS PAGE and Western blot confirmed that the crystalline proteins were Rubisco. Electrofocusing analysis of large and small subunits of Rubisco from G. max and G. soja indicated that both proteins had identical isoelectric focusing patterns consisting of three large and two small subunit polyeptides. |
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| Keywords: | soybean rubisco crystallization IEF-PAGE |
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