基于海洋生物多聚物的功能因子递送系统

基于海洋多聚物的纳米材料是一种新型的功能因子负载体系,临床应用中药物与功能因子的递送方式主要有3种,分别是口服、经皮和注射,但由于体感差、药物递送量低、副作用和有效成分活性丧失等诸多问题的困扰,人们更加希望选择既能提高医疗效果和健康水准,又能满足良好舒适性的传输递送方式。研究发现基于海洋多聚物的纳米材料与传统口服递送体系的系统结合可以实现抗癌药物、营养物质、益生菌、疫苗等功能成分的高效递送,大大提高了上述成分的整体生物利用度。通过阐述纳米载体的制备、功能因子的负载及其吸收机制为基于海洋多聚物功能因子递送体系的发展提供参考和借鉴。     

A Novel Approach for Entrapment of Biopolymers in Silica Matrix by Sol-gel Technique

1 Results The entrapment of biopolymers into silica by the sol-gel technique meets with incompatibility of inorganic and bioorganic components. The aim was to develop a compatible procedure biomimicking the biomineralization processes in the living nature. A suggested solution in Ref.[1-2] for the biopolymer entrapment into silica matrix is based on a novel silica precursor. The developed approach is distinguished from the common technique for fabrication of hybrid biopolymer-silica nanocomposite materi...     

3,4-亚甲二氧基苯甲醛修饰壳聚糖的制备与表征

从内陆螯虾壳中提取甲壳素,对该生物质进行资源化处理得壳聚糖(CTS).微波条件下精制得到高反应活性壳聚糖,脱乙酰度和黏均相对分子质量分别大于84%和3×105.将苯甲醛衍生物作为侧基引入该生物高分子链,研究壳聚糖深度脱乙酰化和生成Schiff碱的反应条件.以精制CTS为原料,以侧基接枝率为指标设计正交试验,筛选出高接枝率3,4-亚甲二氧基苯甲醛(胡椒醛)修饰壳聚糖(Hel-CTS)--(1,4)-2-(3,4-亚甲二氧基-苯甲)-亚胺-2-脱氧-β-D-葡聚糖的合成条件为:反应温度40℃,CTS与胡椒醛质量比为1:2,pH值为6.0,合成时间10 h.对Hel-CTS的结构和性能利用红外光谱(FTIR)、热重分析(TG)和差示扫描量热(DSC)等手段进行了表征.以黏度法结合重量分析法,半定量估算出Hel-CTS的平均相对分子质量为4.97×105.     

光敏性卟啉金属配合物与牛血清白蛋白的结合及性能研究

将难溶于水的meso-四(4-羟基苯基)卟啉锌配合物(ZnTpHPP)与牛血清白蛋白(BSA)结合,制得水溶性高分子金属卟啉配合物BSA-ZnTpHPP-n(n=1,3,5,10).采用紫外-可见光谱、圆二色谱、非变性聚丙烯酰胺凝胶电泳对产物进行了表征.结果表明,锌卟啉与牛血清白蛋白以配位键结合,当BSA与ZnTpHPP以较低比例(1∶1,1∶3)结合时,蛋白结构保持不变.在可见光照射下,考察了BSA-ZnTpHPP-1在BSA-ZnTpHPP-1/MV2+/TEOA光诱导电子转移体系中和甲基紫精(MV2+)的电子转移,表明BSA-ZnTpHPP-1是一种良好的生物高分子光敏剂.     

Polymers with Phosphodiester Bonds：from Models of Biopolymers to Liquid Membranes and Polymerinorganic Hybrids

The backbones of nucleic and teichoic acids are molecules with phosphodiester repeating units with acids）. These can be visualized as follows： built on the poly （alkylene phosphate） chains i. e. macrosix atoms （nucleic acids） or five （1,2- glycerol teichoic     

Preparation of ( )-1-Phenylethylamine by Optical Resolution of Racemic 1-Phenylethylamine with a Chiral Biopolymer,Casein

1 Results It has been reported that crystalline form racemates can be resoluted by various methods[1],but for optical resolution of liquid form racemates,the method is limited in chromatograph.So chiral compounds cannot be obtained on large scale by such method.In our previous paper,( )-3-chloro-1,2-propanediol has been obtained by optical resolution of its racemata (liquid) with a chiral biopolymer,casein[2].     

Microbial production of biopoly esters-poly hydroxyalkanoates

Polyhydroxyalkanoate, abbreviated to PHA, is a family of polyesters produced by a variety of microorganisms. Over 90 PHA with various structure variations have been reported and the number is still increasing. Some interesting physical properties of PHA such as piezoelectricity, nonlinear optical activity and biocompatibility, offer promising applications in areas such as tissue engineering and drug delivery. Worldwide there have been many attempts to produce PHA as biodegradable plastics using various microorganisms obtained from screening natural environments, genetic engineering and mutation methods. It has been found that PHA synthesis is a common phenomenon of bacteria inhabiting in various locations, especially oil-contaminated soils. Bacterial strains capable of synthesizing PHA with various monomers have been identified from oil-contaminated soils, and PHA production strains capable of rapid growth and rapid PHA synthesis on molasses substrate have also been found on molasses contaminated soil. It seems that the combination of novel properties and lower cost will allow easier commercialization of PHA.     

血清中生物高分子活性中心离子分布与高血压病患者血液流变学参数之间的关系

计算高血压患者血清中生物高分子活性中心离子（Ti、V、Cr、Mn、Mo、Zn，Fe、Co、Ni、Cu等过渡金属元素）的分布，发现高血压患者可以分成阴平阳升、阴降阳升、阴平阳降、阴阳两降四型。进一步研究发现这四型患者血清中生物高分子活性中心离子的分布参数与中医高血压各证型（肝阳上亢、阴虚阳亢、肝肾阴虚、阴阳两虚）的血液流变学参数（η低切、η高切、Lb、Hct、ηp）之间有很明显的相关性。所以作者认为生物高分子活性中心离子的分布参数可以作为高血压中医辨证分型的微观定量依据之一。     

海藻酸钠修饰LDH制备多孔炭及其电化学性质

研究分别以Mg-Al-LDH,Zn-Cr-LDH和Mg-Cr-LDH为模板,海藻酸钠为前驱体在600℃下炭化制备的多孔炭发现,多孔炭的BET比表面积从173m2/g增加到497m2/g,而海藻酸钠自身炭化所得多孔炭的比表面积仅为95m2/g。电化学研究表明,以Mg-Cr-LDH为模板制备的多孔炭(PC—4)电极的循环伏安曲线图形更接近矩形,阴极和阳极过程基本对称;在恒电流充放电实验中,50mA/g的电流密度下PC—4电极的电容为92F/g且电流密度为500mA/g时充放电循环1000次后电容损失小于1%。比表面积、孔结构和电化学研究表明,海藻酸钠修饰LDH制备的多孔炭具有作为超级电容器电极材料的潜在价值。     

白蛋白锌卟啉结合体光解水产氢性能

将难溶性的锌卟啉(ZnTpHPP)与牛血清白蛋白(BSA)结合, 制得一类新型水溶性生物高分子金属卟啉配合物(BSA-ZnTpHPP). 通过紫外可见光谱(UV-Vis)、圆二色谱(CD)及非变性聚丙烯酰胺凝胶电泳(Native-PAGE)对 BSA-ZnTpHPP 的结构进行了表征, 发现二者以配位键结合, BSA与锌卟啉以较低比例结合时蛋白质二级结构保持. 考察了BSA-ZnTpHPP的光敏感性, 发现BSA-ZnTpHPP在光照条件下易变成三重激发态, 可以将电子转移给甲基紫精(MV²⁺). 以三乙醇胺(TEOA)为电子供体, 甲基紫精(MV²⁺)为电子中继体, 以BSA-ZnTpHPP/MV²⁺/TEOA/胶体Pt四组分体系考察了BSA-ZnTpHPP的光诱导水解产氢性能, 结果表明, 这类水溶性生物高分子金属卟啉光敏剂具有良好的光解水产氢性能.