Environmental Friendly Route and Wool Shrink Proofing

The shrink proofing on wool with treatment of protease named Argaenzyme STL was studied. The various pretreating auxiliaries, different parameters for protease treating process and the effect of stabilizer were discussed in detail. The varieties of some properties before and after protease treatment were also investigated.     

Modulation of protein biophysical properties by chemical glycosylation: biochemical insights and biomedical implications

Glycosylation constitutes one of the most important posttranslational modifications employed by biological systems to modulate protein biophysical properties. Due to the direct biochemical and biomedical implications of achieving control over protein stability and function by chemical means, there has been great interest in recent years towards the development of chemical strategies for protein glycosylation. Since current knowledge about glycoprotein biophysics has been mainly derived from the study of naturally glycosylated proteins, chemical glycosylation provides novel insights into its mechanistic understanding by affording control over glycosylation parameters. This review presents a survey of the effects that natural and chemical glycosylation have on the fundamental biophysical properties of proteins (structure, dynamics, stability, and function). This is complemented by a mechanistic discussion of how glycans achieve such effects and discussion of the implications of employing chemical glycosylation as a tool to exert control over protein biophysical properties within biochemical and biomedical applications. Received 15 December 2006; received after revision 28 March 2007; accepted 25 April 2007     

构建和谐社会的重要任务:和谐社区建设

社区是进行一定的社会活动、具有互动关系和共同文化维系力的人类群体及其活动区域,大力发展社区建设是社会发展进步的客观要求,也是构建和谐社会的重要保证.要在充分认识和谐社区建设的重要性基础上,按照胡锦涛同志"6·25讲话"精神开拓和激发建设和谐社区的新途径和新活力,为和谐社会建设做出应有的贡献.     

枯草杆菌AS1．398制备中性蛋白酶的研究

本文研究了枯草杆菌ＡＳ１．３９８发酵培养制备中性蛋白酶，对发酵培养基配比，培养条件，金属离子的影响等进行了摇瓶试验。制备的ＡＳ１．３９８中性蛋白酶有如下性质：最适ＰＨ７．２－７．４，在ＰＨ６．５－８．０稳定。最适温度４２－４４℃，３５℃下处理２小时能保持约８５％酶活力，６０℃下１０分钟酶完全失活，此酶被ＥＤＴＡ和Ｃｕ２＋所抑制     

家蚕酯酶同工酶与品种亲缘关系及杂种优势的研究

采用聚丙烯酰胺凝胶垂直板电泳法,测定“东肥”、“华合”、“华合×东肥”、“东肥×华合”、中国种“８１１”、日本种“８１２”、“８１１×８１２”、“８１２×８１１”、“肥激”（东肥经激光处理所得新种）蚕卵和蚕幼虫血液中酯酶同工酶的酶谱,并观察它们的经济性状,结果表明两亲本亲缘关系愈远,则谱带相差愈大,同源性愈弱,杂种一代的酯酶同工酶谱表现出亲本的互补关系,且出现新的“杂种酶带”,其光密度也较高,经济性状较亲本有所提高。经激光处理的品种其基因型已发生变化,其酶谱及经济性状均发生改变。     

两种贮藏温度及药剂处理对藏茵陈种子活力的影响

将川西獐牙菜,椭圆叶花锚,抱茎獐牙菜种子贮存在(18±5℃)和(0～4℃)下18个月。用0 2%CaCl2和2%KNO3溶液浸泡24h,进行电导率、TTC还原量及发芽率测定,结果表明:①两种温度下贮存的种子,除电导率差异极显著外(P<0 01),其余各项指标均不显著(P>0 05);②经药剂处理的种子具有较高的发芽率;两种药剂以0 2%CaCl2效果较好,发芽率平均提高20%;③同一温度贮存的川西獐牙菜和椭圆叶花锚种子,经药剂处理后的电导率极显著高于对照(P<0 01),药剂处理后TTC还原量差异不显著(P>0 05)。     

酶法制备明胶的新工艺研究

阐述了一种酶法制备明胶的新工艺，并对酶用量、酶解温度、酶解时间、溶胶温度、溶胶初始pH值等参数进行了优化。同时，还测定了产品的理化性质。研究表明新工艺生产周期短(7～10d)，环境污染小，产率高。     

泥土芽孢杆菌YMTC1049菌株高温蛋白酶产酶条件的优化

 报道高温蛋白酶产生菌YMTC1049(Geobacillus sp.)产酶条件的优化过程.在基础培养基中分别添加葡萄糖、麦芽糖、酵母提取物、蛋白胨、胰蛋白胨、酪蛋白、聚胨等7种营养成分,获得对酶活影响较大的碳、氮源.用多因素正交试验设计考察了pH、酪蛋白、葡萄糖和温度对酶活的影响水平,菌株在优化发酵条件下培养24h时,上清液蛋白酶活力达312U/(mL·min).     

不同pH 条件下鳜鱼消化道蛋白酶活性研究

初步研究了不同pH条件下鳜鱼(Sinipercachuatsi)消化道不同部位蛋白酶活性的差异.结果表明鳜鱼消化道不同部位蛋白酶的活性存在较大差异,最适pH也不相同.消化道不同部位蛋白酶的活性强弱顺序为:幽门盲囊>胃>肠前段>肠后段.幽门盲囊的蛋白酶活性最强,明显高于胃、肠前段和肠后段.蛋白酶活性在幽门盲囊的最适pH为8 0～10 4,且在偏碱性条件下有较强的活性;在胃部的最适pH约为2 8,且在偏酸性的条件下活性较高;在肠前段和肠后段的最适pH为9 8.     

嗜热蛋白酶的研究与应用

介绍了国内外关于嗜热蛋白酶的研究成果与应用现状，包括产生嗜热蛋白酶的微生物种类，嗜热蛋白酶的特点、稳定机制及基因的克隆策略，嗜热蛋白酶的生产、提取、纯化以及应用．