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LUO HaiYing WANG YunFang KONG Wei PEI XueTao 《科学通报(英文版)》2007,52(18):2449-2456
Today, liver transplantation (LT) is the only established treatment for end-stage liver diseases. The de- velopment of LT, including OLT, cadaveric LT, split LT, living donor LT (LDLT), brings hopes to patients with these diseases. However, increasing donor shortage, rejection and life-long immunosuppression with its side effects are the major limitations of this therapy strategy. Bone marrow-derived stem cells (BMDSCs) are capable of differentiating into hepatocyte-like cells and contribute to liver injury repair. The microenvironment of liver injury caused by rejection, ischemia/reperfusion, loss of liver mass, recurrence of HCV and "small-for-size syndrome" after LT can attract a variety of bone marrow-derived stem cell population to the peripheral circulation and then migration to the injury liver to promote the hepatic function restoration. Additionally, BMDSCs can also take part in the functional regeneration of living donor liver after LDLT. This participation in liver regeneration may be associated to the interac- tion between SDF-1and its receptor CXCR4, involving HGF, IL-8, MMP9, and VEGF/VEGFR-2. BMDSC with its bio-characteristics could maintain the allograft tolerance from different angles and in different ways. In conclusion, BMDSCs transplantation, as a new assistant therapeutic method for LT, will ex- pand the space of LT, and provide more survival opportunities for the patients suffering liver diseases in the future. 相似文献
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研究了新型无铅汽油添加剂甲基叔丁基醚(MTBE)、甲基叔戊基醚(TMAE)对正常人肝细胞系L-02的毒性作用,在较高浓度下,MTBE、TAME均对L-02正常人肝细胞的生长有影响,且TAME的毒性作用稍强,另外,用FT-IR法和^1H-NMR法研究了MTBE、TAME与人血红蛋白的体外作用,结果显示,MTBE、TAME与人血红蛋白无明显的直接作用。 相似文献
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Induction of hemopoietic chimerism in the caprine fetus by intraperitoneal injection of fetal liver cells 总被引:1,自引:0,他引:1
R. D. Pearce D. Kiehm D. T. Armstrong P. B. Little J. W. Callahan L. R. Klunder J. T. R. Clarke 《Cellular and molecular life sciences : CMLS》1989,45(3):307-308
Summary Intraperitoneal injection of allogeneic liver cells from 43-day-old male fetuses into normal 60-day female goat fetuses resulted in persistent hemopoietic chimerism in surviving recipients without clinical evidence of graft-versus-host disease. Transplantation of normal fetal liver cells into preimmunocompetent goat fetuses affected with -D-mannosidosis may provide an alternative strategy for evaluating hemopoietic stem cell transplantation in the treatment of human lysosomal storage diseases. 相似文献
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S. Satoh M. Furuse Y. -H. Choi J. Okumura 《Cellular and molecular life sciences : CMLS》1994,50(9):812-814
To find out whether physiological concentrations of cholecystokinin (CCK), a gastrointestinal hormone in mammals, are also active in chickens, the pancreatic amylase secretory response to CCK-8 was investigated in vitro. Rat pancreatic acini responded to the physiological concentration of CCK-8, but in chickens amylase release was induced at a concentration of CCK-8 1000 times higher than that observed in rats. In another experiment, biliary flow was tested with several doses of CCK-8. The bile flow was stimulated in a dose-dependent fashion, but a significant enhancement was not obtained at a concentration of 0.5 g CCK-8/kg body weight, which was considerably higher than physiological ones. It is concluded that endogeneous CCK does not have an important role in the digestive system in the chicken. 相似文献
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以SPF鸡抗新城疫病毒(NDV)IgG为包被抗体(5μg/mL),兔抗NDVVero细胞培养纯化毒IgG为第二抗体(1∶200~600),酶标记羊抗兔IgG(1∶5000)为指示抗体建立间接夹心ELISA,检测实验免疫鸡、攻毒鸡及现地免疫鸡口腔或泄殖腔外分泌物。结果表明:新城疫无毒力V4疫苗免疫后2d,口腔内病毒抗原随机检出率为4/10,第10天为10/10;泄殖腔为0~1/10,并持续18d以上。滴鼻攻毒后1d,口腔和泄殖腔内病毒抗原检出率分别是8/10和6/10;第13天,泄殖腔内病毒抗原检出阴性。NDV灭活疫苗免疫60d后攻毒,第2天有7/20口腔和8/20泄殖腔检出阳性,并一直到攻毒后第14天。非免疫对照鸡攻毒后第3天直到死亡时,口腔和泄殖腔均可检出病毒抗原。对各地临床上无任何症状的NDV免疫鸡群检测发现,泄殖腔NDV抗原阳性率0%~9.3%,分离到8株NDV流行毒株,生物学试验证实这些毒株的毒力属中等毒力偏强或强毒。 相似文献
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研究白及微球的制作工艺及其猪肝动脉栓塞和临床患者栓塞的效果。方法:采用乳化-冷凝技术制备白及微球,并通过正交实验,确定制备特定粒径微球的最佳工艺条件。通过股动脉经导管把白及微球注入肝动脉。结果:正交试验结果表明所考察的因素如搅拌速度、油/水比例和白及胶浓度对微球粒径及分布均有显著影响。白及微球呈圆形,粒径分布窄,圆整,分散性好,平均粒径为5436μm。实验小猪微球栓塞后的肝动脉造影表明白及微球可致肝右支动脉一级、二级分支完全栓塞;病理检查表明梗死区可见肝组织呈肝硬变改变,大量的假小叶形成。微球临床初步应用效果良好。结论:白及微球作为新型肝动脉栓塞制剂在介入栓塞治疗中有较好的作用 相似文献
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M. Rizzardini A. Ferraroli D. Dal Fiume L. Cantoni 《Cellular and molecular life sciences : CMLS》1984,40(12):1390-1392
Summary Im male rats hepatic cytochromes b5 and P-450 were reduced at different times after treatment with cyclophosphamide (CP) (200 mg/kg i.p. for 3 days). In contrast, microsomal heme did not change until 48 h after the last dose of CP, leading to accumulation of heme in a non-cytochromal form. Parallel to the above changes the heme metabolism showed derangement: -aminolaevulinate synthase, the rate-limiting enzyme in heme synthesis, was depressed and heme oxygenase, the enzyme which catalyzes the oxidative degradation of heme, was increased. 相似文献
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连翘叶茶保肝作用的实验研究 总被引:5,自引:0,他引:5
采用给小鼠腹腔注射四氯化碳制作肝脏损伤模型,通过测定小鼠血清中ALT、AST、CHE、TBIL、TP、GSH和肝脏中SOD、MDA、CHE、ALB、ALT、GSH等指标,研究连翘叶茶对四氯化碳所致小鼠急性肝脏损伤的保护作用.结果表明,连翘叶茶可抑制血清和肝脏中ALT和AST的异常增高,提高血清和肝脏中SOD和CHE的活性,增加GSH、TP和ACB含量,减少MDA形成,调节TBIL代谢.说明连翘叶茶具有明显的保护肝脏的作用。 相似文献