| 大鼠肝ADAMs种类鉴定及肝再生过程中ADAMsmRNA差异分析 |
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| 引用本文： | 林俊堂,李玉昌,梁卫红,张会勇,徐存拴.大鼠肝ADAMs种类鉴定及肝再生过程中ADAMsmRNA差异分析[J].河南科学,2002,20(1):38-41. |
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| 作者姓名： | 林俊堂 李玉昌 梁卫红 张会勇 徐存拴 |
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| 作者单位： | 河南师范大学生命科学学院,河南,新乡,453002 |
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| 基金项目： | 国家自然科学基金项目(39970362)、河南省生物工程重点实验室资助项目 |
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| 摘 要： | 以 2 / 3肝切除 (patialhepatectomy ,PH)大鼠为材料 ,利用PT -PCR技术 ,通过ADAMs通用引物初步分析了肝脏中ADAMs种类及PH后恢复 4h和 36h时ADAMsmRNA差异。结果表明 :PH后不同恢复时间ADAMsmRNA的扩增谱带没有差异 ,但扩增谱带的强弱有变化 ;cDNA克隆和测序分析表明 ,大鼠肝脏有ADAM15和ADAM 19mRNA同源序列。
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| 关 键 词： | 肝再生 ADAMs 通用引物 RT-PCR 基因克隆 |
| 文章编号： | 1004-3918(2002)01-0038-04 |
| 修稿时间： | 2001年11月28 |
Species of ADAMs in rat liver and changes of ADAMs mRNA during liver receneration |
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| LIN Jun-Tang,LIANG Wei-Hong,LI Yu-Chang,XU Cun-Shuan.Species of ADAMs in rat liver and changes of ADAMs mRNA during liver receneration[J].Henan Science,2002,20(1):38-41. |
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| Authors: | LIN Jun-Tang LIANG Wei-Hong LI Yu-Chang XU Cun-Shuan |
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| Abstract: | This article analyzed the species of ADAMs mRNA in rat liver and their changes during liver regeneration after PH using RT-PCR. The results showed that two homogenous sequences with ADAM15 and ADAM19 were checked out in rat liver; that there was no difference of ADAMs mRNA amplified bands in different individuals and sex; that the strong and the weak of the ADAMs mRNA amplified bands was changed in different recovering time after PH. |
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| Keywords: | liver regeneration ADAMs general primer RT-PCR gene clone |
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