正电和中性磷脂对纤维蛋白原的吸附比较

常用纤维蛋白原的吸附特性来比较和研究材料的血液相容性。将自行合成的正电磷脂ＤＰＰＥＬ膜铺展在金膜表面,测量了膜表面的接触角及对纤维蛋白原的动态吸附特性,并与裸金膜、中性磷脂ＤＳＰＣ膜进行了比较。结果表明：ＤＳＰＣ膜、ＤＰＰＥＬ膜和裸金膜的接触角分别为３８°０５′,５７°２０′和７８°２４′。在相同的纤维蛋白原溶液浓度条件下,裸金膜表面吸附纤维蛋白原的速率最快,饱和吸附量最大（３．５０ｎｇ／ｍｍ２）;磷脂ＤＳＰＣ膜表面吸附纤维蛋白原的速度最低,饱和吸附量也最小（１．００ｎｇ／ｍｍ２）;磷脂ＤＰＰＥＬ膜吸附速率也较快,饱和吸附量为１．７５ｎｇ／ｍｍ２。表明纤维蛋白原在界面上的吸附特性与材料表面疏水性质和带电特性相关。ＰＣ头良好的亲水性和电中性,是其能改善生物医用材料血液相容性的重要原因。     

Interaction of SynaptotagminⅠ with Phospholipid Membrane: A Monolayer Study

IntroductionSynaptotagmin is a family of vesicletransmembrane proteins present in synapticvesicles and large secretary granules of neuronsand endocrine cells[1 ] .It is a major constituent ofsynaptic vesicle membranes,comprising7% 8%of the total vesicle protein,characterized by ashort intravesiclar N-terminus,a singletransmembrane region,and a long plasmicdomain.　The best-charaterized form of synaptotagmin,syt ,is found abundantly in rostrol brain.Syt was first described in 1 981 [2 ] ,and i…     

在直拉法尖晶石上外延硅膜的研制

本文叙述了在直拉法生长的尖晶石上外延硅膜的制备与性质。已在直拉法尖晶石 （１００）面衬底上生长出一批电学性能较好的Ｎ型单晶硅膜，并进行了热氧化稳定性试 验，Ｎ型掺杂的硅膜其霍尔迁移率一般在３００厘米２／伏·秒左右．最高达３８０厘米２／伏 ·秒。载流子浓度控制在 １０１５～１０１６／厘米３硅膜厚度在 １—２微米。     

黄瓜光系统Ⅱ捕光叶绿素a/b蛋白复合体二维晶体研究

光系统Ⅱ捕光叶绿素a/b蛋白复合体(Photosystem Ⅱ light-haresting chlorophyll a/b     

膜蛋白研究的里程碑

2003年10月, 诺贝尔化学奖颁给了两位生物学家, 美国约翰霍普金斯大学(Johns Hopkins University)医学院的Peter Agre和洛克菲勒大学(Rockefeller Uni-versity)休斯医学研究所(Howard Hughes Medical In-stitute)的Roderick Mackinnon, 以表彰他们“在膜通道研究领域上的一系列开创性的工作”. Agre的主要贡献是发现了细胞膜上水通道的存在; 而Mackinnon的主要贡献则是通过解析钾离子通道的三维空间结构阐明了该离子通道的机制.     

天花粉蛋白在酸性条件下可自发插入脂单层

天花粉是一种有着悠久应用历史的引产中药，其有效成分花粉蛋白最近被证明是一种核糖体抑制蛋白，它通过切除２８ＳｒＲＮＡ的Ａ４３２４的１个腺嘌呤使核糖体的活而杀伤细胞。     

Cholesterol Depletion Reduces the Internalization of β-Amyloid Peptide in SH-SY5Y Cells

Introduction Alzheimer’s disease (AD) is a degenerative disorder othe human brain. A major pathological hallmark of ADis extracellular amyloid plaque deposited in the cerebral parenchyma and vasculature[1]. Currently, muchattention has been focused on th…     

Multilayers Assembly of DNA Probe for Biosensor

IntroductionThere are many kinds of biosensors for detectingthe interactions of protein-protein,protein-DNAand enzyme-substrates.Surface plasmon resonance(SPR) biosensor is one of the importantdetectingtechniques.The analytical system is based on asensor chip that uses SPR to monitor theinteraction of biomolecules on a sensor chip.SPRis an optical phenomenon,which is sensitive tochanges in the optical properties of the mediumclose to a metal surface[1 ] .This optical techniquemeasures the…     

Surface Plasmon Microscopy and Its Application on Supported Membranes

ＳｕｒｆａｃｅＰｌａｓｍｏｎＭｉｃｒｏｓｃｏｐｙａｎｄＩｔｓＡｐｐｌｉｃａｔｉｏｎｏｎＳｕｐｐｏｒｔｅｄＭｅｍｂｒａｎｅｓ＊ＺｈｏｕＹｕｅ（周悦），ＸｉａｏＣａｉｄｅ（肖才德），ＳｕｉＳｅｎｆａｎｇ（隋森芳）ＤｅｐａｒｔｍｅｎｔｏｆＢｉｏｌｏｇｉｃａ...     

N-Terminal Truncation of TACO Inhibits PMA-Induced U937 Cell Adhesion

The effect of TA001-299, the N-terminal truncation of TACO, on phorbol 12-myristate 13-acetate （PMA）-induced U937 cell adhesion was investigated. Full-length TACO and several truncations were overexpressed in U937 cells. The effects of the expressed proteins on U937 cell adhesion mediated by PMA-induced differentiation were observed by fluorescence microscopy. The results show that the overexpression of TACO1-299 inhibits cell adhesion while overexpressions of the other proteins do not have this effect. The actin-binding capability of TACO1-299 was investigated and the results show that TACO1-299 lacks the ability of TACO to bind F-actin. The inhibitive effect of TACO1-299, the functional domain of TACO, suggests that TACO may play a role in cell differentiation mediating adhesion of monoblastic leukemia cells.