| N-Terminal Truncation of TACO Inhibits PMA-Induced U937 Cell Adhesion |
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| 作者姓名： | 刘长振 隋森芳 |
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| 作者单位： | [1]Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| 基金项目： | Supported by the National Key Basic Research and Development（973） Program of China （No. 2004CB720005） |
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| 摘 要： | The effect of TA001-299, the N-terminal truncation of TACO, on phorbol 12-myristate 13-acetate （PMA）-induced U937 cell adhesion was investigated. Full-length TACO and several truncations were overexpressed in U937 cells. The effects of the expressed proteins on U937 cell adhesion mediated by PMA-induced differentiation were observed by fluorescence microscopy. The results show that the overexpression of TACO1-299 inhibits cell adhesion while overexpressions of the other proteins do not have this effect. The actin-binding capability of TACO1-299 was investigated and the results show that TACO1-299 lacks the ability of TACO to bind F-actin. The inhibitive effect of TACO1-299, the functional domain of TACO, suggests that TACO may play a role in cell differentiation mediating adhesion of monoblastic leukemia cells.
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| 关 键 词： | TACO 细胞支持 遗传表达 U937细胞 |
| 收稿时间： | 2004-12-17 |
| 修稿时间： | 2004-12-172005-01-17 |
N-Terminal Truncation of TACO Inhibits PMA-Induced U937 Cell Adhesion |
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| Changzhen Liu, &#x;,Senfang Sui, î .N-Terminal Truncation of TACO Inhibits PMA-Induced U937 Cell Adhesion[J].Tsinghua Science and Technology,2005,10(4):489-495. |
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| Authors: | Changzhen Liu &#x; Senfang Sui î |
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| Institution: | Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | The effect of TACO1-299, the N-terminal truncation of TACO, on phorbol 12-myristate 13-acetate (PMA)-induced U937 cell adhesion was investigated. Full-length TACO and several truncations were overexpressed in U937 cells. The effects of the expressed proteins on U937 cell adhesion mediated by PMA-induced differentiation were observed by fluorescence microscopy. The results show that the overexpression of TACO1-299 inhibits cell adhesion while overexpressions of the other proteins do not have this effect. The actin-binding capability of TACO1-299 was investigated and the results show that TACO1-299 lacks the ability of TACO to bind F-actin. The inhibitive effect of TACO1-299, the functional domain of TACO, suggests that TACO may play a role in cell differentiation mediating adhesion of monoblastic leukemia cells. |
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| Keywords: | TACO U937 cell differentiation cell adhesion |
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