碳纤维加固低配箍混凝土梁板柱节点的抗震试验

简要介绍了五个用碳纤维加固的混凝土低配箍梁板柱节点低周反复试验的情况 ,对试验结果进行了分析 ,发现加固后的节点极限承载力和抗震性能都有大幅度的提高 ,各项性能指标都达到并超过了我国现行抗震规范的要求 ,因此碳纤维加固混凝土节点技术是一个值得进一步深入研究的课题 .     

Inhibition of Alkaline Phosphatase from Pearl Oyster Pinctada fucata by o-Phthalaldehyde： Involvement of Lysine and Histidine Residues at the Active Site

Alkaline phosphatase from Pinctada fucata was inactivated by o-phthalaldehyde （OPA）. The inactivation followed pseudo first-order kinetics with a second rate constant of 0.167 （mmol/L）^-1·min^-1 at pH 7.5 and 25℃. A Tsou＇s plot analysis showed that inactivation occurred upon formation of one isoindole group. The OPA-modified enzyme lost the ability to bind with the specific affinity column and the presence of substrates or competitive inhibitors protected the enzyme from inactivation. The results revealed that the OPA-reaction site was at the enzyme substrate binding site. Prior modification of the enzyme by lysine or histidine specific reagent abolished formation of the isoindole derivatives, suggesting that lysine and histidine residues were involved in the OPA-induced inactivation. Taken together, OPA inactivated the alkaline phosphatase from Pinctada fucata by cross-linking lysine and histidine residues at the active site and formed an isoindole group at the substrate binding site of the enzyme.     

Effects of Genistein and Daidzein on the Proliferation, Invasion, Migration and Adhesion of Melanoma Cells

IntroductionGenistein and daidzein (Fig.1 ) ,two majorisoflavonoids in soybeans,were reported to playimportant roles in cancer prevention[1 ,2 ] .Previousstudies demonstrated that genistein and daidzeininhibited the growth of leukemia,breast,colonand prostate cancers[38] .Our earlier studies alsodemonstrated that daidzein enhanced the immunefunction in mice[9,1 0 ] . These functions madegenistein and daidzein promising candidates forcancer prevention. However,it is usually themetastatic disse…     

Multiple Suppressive Effects of a Protein from Caesalpinia minax on Murine Melanoma Cells

IntroductionIn recent years,a great deal of attention hasfocused on finding potential anti- tumor agentsfrom natural sources[14 ] .A vast number ofpromising candidate molecules,especiallycomponents extracted from plants,have beenevaluated[59] .　 Herbs have a long history of use as Chinesetraditional medicine.Caesalpinia minax (C.minax) is a wild plantin Yunnan Province,China.The high level of ultraviolet radiation in thistropical province causes widespread skin diseases inthis area.Extract…     

有序介孔硅MCM-41对美洛昔康的包载及其形成的复合物MCM41-MX的溶出特征

通过有序介孔硅基材料MCM-41来增加非甾体抗炎药美洛昔康的溶出速率.采用紫外分光光度法、热重分析、XRD分析与差热扫描进行了表征,并对复合物体外溶出进行了实验.结果表明:美洛昔康与MCM-41制成的复合物的载药量为10%左右;复合物中的美洛昔康不是以晶体形式存在,药物分子与硅羟基之间有氢键作用;在pH=7.4缓冲溶液中,复合物与美洛昔康原料药和物理混合物相比,溶出度得到显著改善.     

14MeV快中子辐照对大鼠免疫系统的损伤

用14 MeV快中子对Wistar雄性大鼠进行5 Gy的全身照射,观察其免疫系统损伤情况及氧化应激的相关变化.大鼠分为辐射组和对照组,分别在辐射后1,7,14d 3个时间点取血液、胸腺和脾脏进行研究.结果表明,辐照后第1d,辐照组大鼠白细胞和淋巴细胞数量降至最低,照后第7、14d有所恢复,但仍不足正常组的1/2.血小板数在照后第1d开始减少,至第7d达到最低值,第14d仍与正常组有显著差异.而大鼠骨髓有核细胞总数辐照后14d明显低于正常组.另外胸腺和脾脏均出现萎缩,脏器指数明显减小.血浆、胸腺和脾脏的总抗氧化能力T-AOC、SOD酶活性及MDA值含量均有不同程度的升高趋势.说明快中子辐射影响血浆、胸腺和脾脏的抗氧化应激能力,诱导细胞凋亡或坏死,造成胸腺和脾脏的萎缩以及一系列组织形态的改变,从而严重影响大鼠的正常免疫功能.      

Cloning, Characterization, and Expression Analysis of Calreticulin from Pearl Oyster Pinctada fucata

Calreticulin is a unique calcium-binding protein with multiple functions mostly located in the sarcoplasmic/endoplasmic reticulum. A large amount of calcium is absorbed from the medium and transported to mineralization sites during biomineralization in pearl oyster. This paper describes the cloning of the full-length cDNA of calreticulin from Pinctada fucata, namely PCRT. PCRT encodes a deduced 414-amino acid protein, which includes a predicted 17- amino acid signal peptide and an endoplasmic reticulum retrieval sequence HDEL. The protein shows 63%-76% sequence identity and shares some common characteristics with calreticulins from other species. Semi-quantitative RT-PCR indicates that PCRT is ubiquitously expressed in all tissues tested with the highest expression in the hemolymph and the mantle. In situ hybridization analysis of PCRT in the mantle showed strong signals in the inner fold, the inner side of middle fold, and the inner side of outer fold of the mantle epithelium, All these results suggest PCRT might be involved in Ca^2＋ transport and storage during oyster biomineralization.     

物理化学开放设计性实验教学的探索与实施

在药学专业物理化学实验教学中开设了开放设计性实验内容,探索了物理化学开放设计性实验的实施过程.通过物理化学实验教学新模式的实践,提高了学生学习的主动性、参与性和创造性,达到了开放设计性实验的教学效果.     

Detection of Messenger RNA for Gonadotropin-Releasing Hormone (GnRH) but not for GnRH Receptors in Rat Pancreas

IntroductionGonadotropin- releasing hormone ( Gn RH) ,whichis synthesized in the brain and secreted into theanterior pituitary,plays a central role in thecontrol of mammalian reproduction[1,2 ] .Gn RH andGn RH m RNA have been reported to exist in non-hypothalamic tissues such as ovary[3,4 ] ,breast[5] ,testes,and placenta[6] .The Gn RH binding siteand its m RNA have also been found inextrapituitary tissues,including gonads andplacenta[7] .These findings indicate the biologicalsignifican…