Multiple Suppressive Effects of a Protein from Caesalpinia minax on Murine Melanoma Cells

IntroductionIn recent years,a great deal of attention hasfocused on finding potential anti- tumor agentsfrom natural sources[14 ] .A vast number ofpromising candidate molecules,especiallycomponents extracted from plants,have beenevaluated[59] .　 Herbs have a long history of use as Chinesetraditional medicine.Caesalpinia minax (C.minax) is a wild plantin Yunnan Province,China.The high level of ultraviolet radiation in thistropical province causes widespread skin diseases inthis area.Extract…     

Genistein and Daidzein Effects on Proliferation, Cell Membranes, Cell Cycles and Cell Apoptosis of Different Cell Lines

IntroductionIsoflavonoids are found mainly in soybeans,aprimary food source for humans and herbivores.The structure and function of isoflavonoids aresimilar to 1 7β- estradiol (1 7β- E2 ) ,and have dualeffects as both mammal oestrogenicity and anti-oestrogenicity,so they are also termed isoflavonicphytoestrogens(IPE) .It has been reported thatthese compounds can reduce blood fat,as well asthe risk of cancer and osteoporosis[1] .Genistein and daidzein are two principleisoflavonoids in soybe…     

试论南方局的党员先进性教育

南方局的党员先进性教育独树一帜,卓有成效,有许多地方值得今天的党员先进性教育借鉴.     

重庆市国有文化资产管理体制探讨

如何有效地管理国有文化资产,是一个全新的课题.本文审时度势,在深入研究的基础上,开创性地提出了建构具有重庆地方特色的国有文化资产管理体制应坚持的基本原则、总目标、具体任务以至新一轮文化体制改革应选择的正确路径.     

Effects of Genistein on Cell Cycle and Apoptosis of Two Murine Melanoma Cell Lines

The effects of genistein on several tumor cell lines were investigated to study the effects of gen- istein on cell growth, cell cycle, and apoptosis of two murine melanoma cell lines, B16 and K1735M2. These two closely related murine melanoma cell lines, however, have different responses to the genistein treat- ment. Genistein inhibits the growth of both the B16 and K1735M2 cell lines and arrests the growth at the G2/M phase. After treatment with 60 μmol/L genistein for 72 h, apoptosis and caspase activities were de- tected in B16 cells, while such effects were not found in K1735M2. Further tests showed that after genistein treatment the protein content and mRNA levels of p53 increased in B16, but remained the same in K1735M2. The protein content and mRNA levels of p21WAF1/CIP1 increased in both cell lines after treatment. The results show that genistein might induce apoptosis in B16 cells by damaging the DNA, inhibiting topoi- somerase II, increasing p53 expression, releasing cytochrome c from the mitochondria, and activating the caspases which will lead to apoptosis.